![Western blot - BAG2 antibody [EPR3567] (ab79406) image](#BAG2-Primary-antibodies-ab79406-1.jpg)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - BAG2 antibody [EPR3567] (ab79406) image](#BAG2-Primary-antibodies-ab79406-2.jpg)
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Products:Cell Biology >> Apoptosis >> Intracellular >> Bcl2 Family
Overview
Product name
Anti-BAG2 antibody [EPR3567]
See all BAG2 products (7) ...
Description
Rabbit monoclonal [EPR3567] to BAG2
Tested applications
WB, IP, IHC-P, ICC, Flow Cytmore details
Cross reactivity
Reacts with
Human
Does not react with
Mouse, Rat
Immunogen
A synthetic peptide corresponding to residues in the N terminus of human BAG2
Positive control
Jurkat, HeLa and HepG2 cell lysates; human ovarian carcinoma tissue.
General notes
Produced under U.S. Patent No. 5,675,063.
Properties
Form
Liquid
Storage instructions
Store at -20°C. Stable for 12 months at -20°C
Storage buffer
Preservative: 0.01% Sodium Azide
Constituents: 40% Glycerol, 0.05% BSA, Tissue culture supernatant, 0.15M Sodium chloride, 50mM Tris glycine, pH 7.4
Purity
Tissue culture supernatant
Clonality
Monoclonal
Clone number
EPR3567
Isotype
IgG
Research areas
Cancer >> Invasion/microenvironment >> Apoptosis >> Bcl 2 family
Signal Transduction >> Protein Trafficking >> Chaperones >> Other Chaperones
Cell Biology >> Apoptosis >> Intracellular >> Bcl2 Family
Applications
Show applications key
Our Abpromise guarantee covers the use of ab79406 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notes
Flow Cyt: 1/60.
ICC: 1/100 - 1/250.
IHC-P: 1/100. Antigen retrieval is recommended.
IP: 1/10.
WB: 1/1000 - 1/5000. Detects a band of approximately 24 kDa (predicted molecular weight: 24 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Inhibits the chaperone activity of HSP70/HSC70 by promoting substrate release.
Sequence similarities
Contains 1 BAG domain.
Target information above from: UniProt accessionO95816
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- BAG 2 antibody
- BAG family molecular chaperone regulator 2 antibody
- BAG-2 antibody
see all
Database links
- Entrez Gene: 9532 Human
- Omim: 603882 Human
- SwissProt: O95816 Human
- Unigene: 55220 Human
- Unigene: 729098 Human
Anti-BAG2 antibody [EPR3567] images:
Western blot - BAG2 antibody [EPR3567] (ab79406)
![Western blot - BAG2 antibody [EPR3567] (ab79406)](/ps/datasheet/images/79/ab79406/BAG2-Primary-antibodies-ab79406-1.jpg)
All lanes : Anti-BAG2 antibody [EPR3567] (ab79406) at 1/5000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : HepG2 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size : 24 kDa
Observed band size : 24 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - BAG2 antibody [EPR3567] (ab79406)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - BAG2 antibody [EPR3567] (ab79406)](/ps/datasheet/images/79/ab79406/BAG2-Primary-antibodies-ab79406-2.jpg)
ab79406, at 1/100 dilution, staining BAG2 in human ovarian carcinoma by Immunohistochemistry using paraffin-embedded tissue.
References for Anti-BAG2 antibody [EPR3567] (ab79406)
ab79406 has not yet been referenced specifically in any publications.
Publishing research using ab79406? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"