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Western blot - BAG2 antibody [EPR3567] (ab79406)
![Western blot - BAG2 antibody [EPR3567] (ab79406)](/ps/datasheet/images/79/ab79406/BAG2-Primary-antibodies-ab79406-1.jpg)
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - BAG2 antibody [EPR3567] (ab79406)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - BAG2 antibody [EPR3567] (ab79406)](/ps/datasheet/images/79/ab79406/BAG2-Primary-antibodies-ab79406-2.jpg)
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Product Name
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BAG2 antibody [EPR3567]
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See all BAG2 antibodies (6)...
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Product type
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Primary antibodies
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Description
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Rabbit monoclonal [EPR3567] to BAG2
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Immunogen
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A synthetic peptide corresponding to residues in the N terminus of human BAG2
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Reacts with
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Hu
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Does not react with
(species key)
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Ms, Rat
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Tested applications
(see key)
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Flow Cyt, ICC, IHC-P, IP, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
Flow Cyt: 1/60.
ICC: 1/100 - 1/250.
IHC-P: 1/100. Antigen retrieval is recommended.
IP: 1/10.
WB: 1/1000 - 1/5000. Detects a band of approximately 24 kDa (predicted molecular weight: 24 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Jurkat, HeLa and HepG2 cell lysates; human ovarian carcinoma tissue.
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Research areas
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Cancer >> Invasion/microenvironment >> Apoptosis >> Bcl 2 family
Signal Transduction >> Protein Trafficking >> Chaperones >> Other Chaperones
Cell Biology >> Apoptosis >> Intracellular >> Bcl2 Family
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Relevance
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BAG proteins compete with Hip for binding to the Hsc70/Hsp70 ATPase domain and promote substrate release. All the BAG proteins have an approximately 45-amino acid BAG domain near the C terminus but differ markedly in their N-terminal regions. The predicted BAG2 protein contains 211 amino acids. The BAG domains of BAG1, BAG2, and BAG3 interact specifically with the Hsc70 ATPase domain in vitro and in mammalian cells. All 3 proteins bind with high affinity to the ATPase domain of Hsc70 and inhibit its chaperone activity in a Hip-repressible manner.
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BAG2 antibody [EPR3567] (ab79406) |
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Monoclonal
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Clone number
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EPR3567
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Isotype
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IgG
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Purity
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Tissue culture supernatant
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Storage buffer
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Preservative: 0.01% Sodium Azide
Constituents: 40% Glycerol, 0.05% BSA, Tissue culture supernatant, 0.15M Sodium chloride, 50mM Tris glycine, pH 7.4 Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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Concentration not determined - why is this?
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Storage instructions
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Store at -20°C. Stable for 12 months at -20°C
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Notes
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Produced under U.S. Patent No. 5,675,063.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this BAG2 antibody [EPR3567] is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for BAG2 antibody [EPR3567] images, references, products related to ab79406 and other tools.
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BAG2 antibody [EPR3567] images:
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 Western blot - BAG2 antibody [EPR3567] (ab79406)
All lanes : BAG2 antibody [EPR3567] (ab79406) at 1/5000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : HepG2 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size : 24 kDa
Observed band size : 24 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - BAG2 antibody [EPR3567] (ab79406)
ab79406, at 1/100 dilution, staining BAG2 in human ovarian carcinoma by Immunohistochemistry using paraffin-embedded tissue.
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Search PubMed (MEDLINE) for references to BAG2
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BAG2 antibody [EPR3567] - more information
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Customer reviews (feedback) regarding BAG2 antibody [EPR3567] |
Customer FAQs regarding BAG2 antibody [EPR3567] |
Protocols for BAG2 antibody [EPR3567] |
Price and availability of products related to BAG2 antibody [EPR3567] |
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BAG2 antibody [EPR3567] - related products:
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BAG2 antibody [EPR3567] - other tools:
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Contact Abcam with a Technical Enquiry about BAG2 antibody [EPR3567] |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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![Western blot - BAG2 antibody [EPR3567] (ab79406)](/index.html?datasheet=79406#BAG2-Primary-antibodies-ab79406-1.jpg)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - BAG2 antibody [EPR3567] (ab79406)](/index.html?datasheet=79406#BAG2-Primary-antibodies-ab79406-2.jpg)
