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Western blot - BAG2 antibody (ab47106)
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Immunocytochemistry/ Immunofluorescence - BAG2 antibody (ab47106)
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Product Name
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BAG2 antibody
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See all BAG2 antibodies (6)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to BAG2
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Immunogen
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Recombinant full length protein (Human)
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Reacts with
(species key)
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Hu
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Tested applications
(see key)
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ICC/IF, IHC-Fr, IHC-P, IP, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ICC/IF: 1/1000 - 1/5000.
IHC-Fr: Use at an assay dependent dilution.
IHC-P: 1/1000 - 1/5000.
IP: 1/50 - 1/200.
WB: 1/1000 - 1/2000. Detects a band of approximately 24 kDa (predicted molecular weight: 24 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Lymphoid, and many cancer cell lines. HeLa cells.
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Research areas
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Cancer >> Invasion/microenvironment >> Apoptosis >> Bcl 2 family
Signal Transduction >> Protein Trafficking >> Chaperones >> Other Chaperones
Cell Biology >> Apoptosis >> Intracellular >> Bcl2 Family
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Relevance
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BAG proteins compete with Hip for binding to the Hsc70/Hsp70 ATPase domain and promote substrate release. All the BAG proteins have an approximately 45-amino acid BAG domain near the C terminus but differ markedly in their N-terminal regions. The predicted BAG2 protein contains 211 amino acids. The BAG domains of BAG1, BAG2, and BAG3 interact specifically with the Hsc70 ATPase domain in vitro and in mammalian cells. All 3 proteins bind with high affinity to the ATPase domain of Hsc70 and inhibit its chaperone activity in a Hip-repressible manner.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Whole antiserum
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Storage buffer
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Preservative: 0.05% Sodium Azide
Constituents: Whole serum Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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Concentration not determined - why is this?
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this BAG2 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for BAG2 antibody images, references, products related to ab47106 and other tools.
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BAG2 antibody images:
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 Western blot - BAG2 antibody (ab47106)
BAG2 antibody (ab47106) at 1/2000 dilution + HeLa cells
Predicted band size : 24 kDa
Observed band size : 24 kDa
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Immunocytochemistry/ Immunofluorescence - BAG2 antibody (ab47106)
Immunofluorescence confocal microscopy of BAG-2 using ab47106 at 1:2000 on HeLa cells growing on coverslips left untreated (A) or heat shocked at 42 degrees for 30 min (B). Cells were fixed with 3.7% paraformaldehyde, permeabilized with 0.5% Triton X-100 prior to antibody staining. BAG2 localized to the cytoplasm of untreated cells, and to the nucleus of cells subjected to heat shock.
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Search PubMed (MEDLINE) for references to BAG2
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BAG2 antibody - more information
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Customer reviews (feedback) regarding BAG2 antibody |
Customer FAQs regarding BAG2 antibody |
Protocols for BAG2 antibody |
Price and availability of products related to BAG2 antibody |
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BAG2 antibody - related products:
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BAG2 antibody - other tools:
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Contact Abcam with a Technical Enquiry about BAG2 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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