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Products:Cell Biology >> Apoptosis >> Intracellular >> Bcl2 Family
Overview
Product name
Anti-BAG2 antibody
See all BAG2 products (7) ...
Description
Rabbit polyclonal to BAG2
Tested applications
WB, IHC-P, IHC-Fr, IP, ICC/IFmore details
Cross reactivity
Reacts with
Human
Immunogen
Recombinant full length protein (Human)
Positive control
Lymphoid, and many cancer cell lines. HeLa cells.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.05% Sodium Azide
Constituents: Whole serum
Purity
Whole antiserum
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Invasion/microenvironment >> Apoptosis >> Bcl 2 family
Signal Transduction >> Protein Trafficking >> Chaperones >> Other Chaperones
Cell Biology >> Apoptosis >> Intracellular >> Bcl2 Family
Applications
Show applications key
Our Abpromise guarantee covers the use of ab47106 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/1000 - 1/2000.Detects a band of approximately 24 kDa (predicted molecular weight: 24 kDa).
IHC-P
IHC-P: 1/1000 - 1/5000.IHC-P: 1/1000 - 1/5000.
IHC-Fr: Use at an assay dependent dilution.
IP
IP: 1/50 - 1/200.IP: 1/50 - 1/200.
ICC/IF: 1/1000 - 1/5000.
Target
Function
Inhibits the chaperone activity of HSP70/HSC70 by promoting substrate release.
Sequence similarities
Contains 1 BAG domain.
Target information above from: UniProt accessionO95816
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- BAG 2 antibody
- BAG family molecular chaperone regulator 2 antibody
- BAG-2 antibody
see all
Database links
- Entrez Gene: 9532 Human
- Omim: 603882 Human
- SwissProt: O95816 Human
- Unigene: 55220 Human
- Unigene: 729098 Human
Anti-BAG2 antibody images:
Western blot - BAG2 antibody (ab47106)
Anti-BAG2 antibody (ab47106) at 1/2000 dilution + HeLa cells
Predicted band size : 24 kDa
Observed band size : 24 kDa
Immunocytochemistry/ Immunofluorescence - BAG2 antibody (ab47106)
Immunofluorescence confocal microscopy of BAG-2 using ab47106 at 1:2000 on HeLa cells growing on coverslips left untreated (A) or heat shocked at 42 degrees for 30 min (B). Cells were fixed with 3.7% paraformaldehyde, permeabilized with 0.5% Triton X-100 prior to antibody staining. BAG2 localized to the cytoplasm of untreated cells, and to the nucleus of cells subjected to heat shock.
References for Anti-BAG2 antibody (ab47106)
ab47106 has not yet been referenced specifically in any publications.
Publishing research using ab47106? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"