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Products:Epigenetics and Nuclear Signaling >> Transcription >> Other factors
This fast track antibody is not yet fully characterised. It is subject to these terms and conditions
Overview
Product name
Anti-BAHD1 antibody
See all BAHD1 products (2) ...
Description
Rabbit polyclonal to BAHD1
This product is a fast track antibody. It has been affinity purified and shows high titre values against the immunizing peptide by ELISA. Read the terms of use »
Cross reactivity
Predicted to work with
Mouse, Human
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 400 - 500 of Human BAHD1.
(Peptide available as ab66437.)
Positive control
This antibody gave a positive signal in the following whole cell lysates: HeLa, Jurkat and HEK293
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
-
Western blot - BAHD1 antibody (ab46573)
(enlarge)
Applications
Application notes
This antibody gave a positive result in ELISA against the immunizing peptide (ab66437).
Optimal dilutions/concentrations should be determined by the end user.
Target
Relevance
BAHD1 (bromo adjacent homology domain-containing 1 protein) is, as its name suggests, a bromo-adjacent homology (BAH) domain. The BAH domain is found in eukaryotic DNA (cytosine-5) methyltransferases, the origin recognition complex 1 (Orc1) proteins, and several proteins involved in transcriptional regulation. It is thought to function as a protein-protein interaction domain. The BAH domain of yeast Sir3p has been shown to bind to nucleosomes and to the N-terminal tail of histone H3.
Cellular localization
Nucleus. Note: Localizes to heterochromatin and inactive X chromosome. Colocalizes with histone H3 trimethylated at 'Lys-27' (H3K27me3).
Alternative names
- AL022997 antibody
- AW541238 antibody
- BAH domain containing protein 1 antibody
see all
Database links
- Entrez Gene: 22893 Human
- Entrez Gene: 228536 Mouse
- SwissProt: Q8TBE0 Human
- SwissProt: Q497V6 Mouse
- Unigene: 22109 Human
- Unigene: 330487 Mouse
Anti-BAHD1 antibody images:
This Fast-Track antibody is not yet fully characterised. These images represent inconclusive preliminary data.
Western blot - BAHD1 antibody (ab46573)
All lanes : Anti-BAHD1 antibody (ab46573) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 3 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size : 85 kDa
Observed band size : 75 kDa (why is the actual band size different from the predicted?)
ab46573 detects a band at 75 kDa in a range of human cell lysates. We are unsure as to why the band seen is lower than predicted and would welcome any customer feedback and comments regarding this product and the data presented above.
References for Anti-BAHD1 antibody (ab46573)
ab46573 has not yet been referenced specifically in any publications.
Publishing research using ab46573? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"