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Please read the definition of 'Fast-Track antibody'

Western blot - BAHD1 antibody (ab46573)
Western blot - BAHD1 antibody (ab46573)
(enlarge)


This Fast-Track antibody is not yet fully characterised. These images represent inconclusive preliminary data.

Product Name 

BAHD1 antibody

Product type 

Primary antibodies

Description 

Rabbit polyclonal to BAHD1

 

  Note on antibody grade

Fast-Track antibodies

  • Immunogen affinity purified
  • high ELISA titre values
  • released early (to accelerate your research)
  • 950 Abpoints to the 1st researcher
    who submits conclusive data (positive or negative):
    Read the Fast-Track antibody Terms of Use


Immunogen 

Synthetic peptide conjugated to KLH derived from within residues 400 - 500 of Human BAHD1.

(Peptide available as ab66437.)

Predicted to react 
(species key)

Hu
(Due to sequence homology)


Abreviews 

Customer reviews
(see key)


Read about Abreviews

Submit an Abreview

Be the first to review this antibody and earn 950 Abpoints.


Read Fast Track antibody terms and conditions.


Application notes 
(see key)

Recommended dilutions
This antibody gave a positive result in ELISA against the immunizing peptide (ab66437).
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

This antibody gave a positive signal in the following whole cell lysates:
HeLa, Jurkat and HEK293

Cellular localization 

Nuclear

Research areas 

Chromatin and Nuclear Signaling >> Transcription >> Transcription Factors
Chromatin and Nuclear Signaling >> Transcription >> Other factors

Relevance 

BAHD1 (bromo adjacent homology domain-containing 1 protein) is, as its name suggests, a bromo-adjacent homology (BAH) domain. The BAH domain is found in eukaryotic DNA (cytosine-5) methyltransferases, the origin recognition complex 1 (Orc1) proteins, and several proteins involved in transcriptional regulation. It is thought to function as a protein-protein interaction domain. The BAH domain of yeast Sir3p has been shown to bind to nucleosomes and to the N-terminal tail of histone H3.

 

Alternative names BAHD1 antibody (ab46573)

Database links 

The links below go to external sites and will open in a new browser window

Entrez Gene

    

22893    (Human)
228536    (Mouse)

SwissProt

    

Q8TBE0    (Human)
Q497V6    (Mouse)

Unigene

    

22109    (Human)
330487    (Mouse)

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

 

At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this BAHD1 antibody is on this datasheet. But please do contact us if you would like any reassurance!


See below for BAHD1 antibody images, references, products related to ab46573 and other tools.

 

BAHD1 antibody images:

This Fast-Track antibody is not yet fully characterised. These images represent inconclusive preliminary data.
Please read the definition of 'Fast-Track antibody'

 

Product image  Western blot - BAHD1 antibody (ab46573)

Western blot - BAHD1 antibody (ab46573)

All lanes : BAHD1 antibody (ab46573) at 1 µg/ml

Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 3 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate

Lysates/proteins at 10 µg per lane.

Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

Performed under reducing conditions.

Predicted band size : 85 kDa
Observed band size : 75 kDa (why is the actual band size different from the predicted?)


ab46573 detects a band at 75 kDa in a range of human cell lysates. We are unsure as to why the band seen is lower than predicted and would welcome any customer feedback and comments regarding this product and the data presented above.

 
 

Search PubMed (MEDLINE) for references to BAHD1

 

BAHD1 antibody - more information

Customer reviews (feedback) regarding BAHD1 antibody

Customer FAQs regarding BAHD1 antibody

Protocols for BAHD1 antibody

Price and availability of products related to BAHD1 antibody

 

BAHD1 antibody - related products:

 

Immunizing Peptide for BAHD1 antibody

ab66437

  

BAHD1 peptide

 

Compatible Secondaries for BAHD1 antibody

ab6564

  

Cy5 ® antibody

ab6717

  

FITC antibody

ab6720

  

Biotin antibody

ab6721

  

HRP antibody

ab6722

  

Alkaline Phosphatase antibody

ab6793

  

Texas Red ® antibody

ab7007

  

Phycoerythrin antibody

ab7019

  

Agarose antibody

ab27234

  

10nm Gold antibody

ab27235

  

5nm Gold antibody

ab27236

  

15nm Gold antibody

ab27237

  

20nm Gold antibody

ab27238

  

30nm Gold antibody

ab27239

  

10nm Gold antibody

ab27240

  

5nm Gold antibody

ab31193

  

Duck polyclonal to Rabbit IgG

ab60314

  

Chromeo™ 488 antibody

ab60315

  

Chromeo™ 494 antibody

ab60317

  

Chromeo™ 546 antibody

ab60319

  

Chromeo™ 642 antibody

 

Other BAHD1 Antibodies

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BAHD1 antibody - other tools:

Contact Abcam with a Technical Enquiry about BAHD1 antibody

 

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