Anti-BAIAP2L1/IRTKS antibody (ab226344)
Key features and details
- Rabbit polyclonal to BAIAP2L1/IRTKS
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-BAIAP2L1/IRTKS antibody
See all BAIAP2L1/IRTKS primary antibodies -
Description
Rabbit polyclonal to BAIAP2L1/IRTKS -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Chimpanzee, Cynomolgus monkey, Rhesus monkey, Gorilla, Orangutan -
Immunogen
Synthetic peptide within Human BAIAP2L1/IRTKS aa 461-511. The exact sequence is proprietary.
Database link: Q9UHR4 -
Positive control
- WB: HeLa and HEK-293T whole cell lysates. IP: HEK-293T whole cell lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7-8 -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab226344 was affinity purified using an epitope specific to BAIAP2L1/IRTKS immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab226344 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/2000 - 1/10000. Predicted molecular weight: 57 kDa.
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IP |
Use at 2-10 µg/mg of lysate.
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Notes |
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WB
1/2000 - 1/10000. Predicted molecular weight: 57 kDa. |
IP
Use at 2-10 µg/mg of lysate. |
Target
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Function
May function as adapter protein. Involved in the formation of clusters of actin bundles. Plays a role in the reorganization of the actin cytoskeleton in response to bacterial infection. -
Sequence similarities
Contains 1 IMD (IRSp53/MIM homology) domain.
Contains 1 SH3 domain. -
Domain
The IMD domain is predicted to have a helical structure. It may induce actin bundling and filopodia formation. -
Post-translational
modificationsPhosphorylated on tyrosine in response to insulin. -
Cellular localization
Cytoplasm, cytoskeleton. Recruited to actin pedestals that are formed upon infection by bacteria at bacterial attachment sites. - Information by UniProt
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Database links
- Entrez Gene: 463559 Chimpanzee
- Entrez Gene: 101129311 Gorilla
- Entrez Gene: 55971 Human
- Omim: 611877 Human
- SwissProt: Q9UHR4 Human
- Unigene: 656063 Human
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Alternative names
- BAI1 associated protein 2 like 1 antibody
- BAI1 associated protein 2 like protein 1 antibody
- BAI1-associated protein 2-like protein 1 antibody
see all
Images
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All lanes : Anti-BAIAP2L1/IRTKS antibody (ab226344) at 0.1 mg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 57 kDa
Exposure time: 30 secondsCells prepared using NETN lysis buffer.
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BAIAP2L1/IRTKS was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab226344 at 6 µg per reaction. Western blot was performed from the immunoprecipitate using ab226344 at 1 µg/ml.
Lane 1: ab226344 IP in HEK-293T whole cell lysate.
Lane 2: Control IgG IP in HEK-293T whole cell lysate.
Detection: Chemiluminescence with exposure time of 30 seconds.
Cells prepared using NETN lysis buffer.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab226344 has been referenced in 2 publications.
- Wang S et al. Upregulated insulin receptor tyrosine kinase substrate promotes the proliferation of colorectal cancer cells via the bFGF/AKT signaling pathway. Gastroenterol Rep (Oxf) 9:166-175 (2021). PubMed: 34026224
- Yuan G et al. Upregulated circRNA_102231 promotes gastric cancer progression and its clinical significance. Bioengineered 12:4936-4945 (2021). PubMed: 34374630