BALB/3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate (ab7901)

Overview

  • Product nameBALB/3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
  • General notesCell line: BALB/3T3 clone A31 (Mouse embryo) Growth media: DMEM & 10% FBS (Fetal bovine serum)
  • Tested applicationsWB more details

Properties

  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 5% Beta mercaptoethanol, 1X SDS
  • Concentration information loading...
  • Lysate notes BALB/3T3 clone A31 cell lysate was prepared by homogenization in modified RIPA buffer(150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl flouride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 µg/ml of aprotinin, 5 µg/ml of leupeptin). Cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The cell lysate was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol.
  • Research Areas

    Applications

    Our Abpromise guarantee covers the use of ab7901 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    WB Use at an assay dependent dilution. Ready to load; 20 µg/lane is recommended for mini gel.

    References for BALB/3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate (ab7901)

    ab7901 has not yet been referenced specifically in any publications.

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    Thank you very much for contacting us with your enquiry.

    I'm sorry to hear that there were no bands in Western blot using this antibody. I would expect any of the MEF cell lines to work as a positive control, but I suggest using a whole cell ...

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    Thank you for contacting Abcam and for your interest in our whole cell lysates.

    Regarding these products, they are not tested for protein composition. They are only tested for protein concentration and confirmed to not be degraded.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"