Recombinant
RabMAb

Anti-BANF1 antibody [EPR7668] (ab129184)

Overview

  • Product name
    Anti-BANF1 antibody [EPR7668]
    See all BANF1 primary antibodies
  • Description
    Rabbit monoclonal [EPR7668] to BANF1
  • Tested applications
    Suitable for: WB, IHC-P, Flow Cyt, ICC/IFmore details
    Unsuitable for: IP
  • Species reactivity
    Reacts with: Mouse, Rat, Dog, Human
  • Immunogen

    Synthetic peptide corresponding to residues in Human BANF1 (O75531).

  • Positive control
    • WB: HeLa, HepG2, and 293T cell lysates. IHC-P: Human colonic carcinoma tissue. ICC/IF: HeLa and NIH/3T3 cells. Flow Cyt: HeLa cells.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Dissociation constant (KD)
    KD = 2.18 x 10 -10 M
    Learn more about KD
  • Storage buffer
    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 49% PBS, 50% Glycerol, 0.05% BSA
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    EPR7668
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab129184 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 10 kDa.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt 1/200.
ICC/IF 1/100.
  • Application notes
    Is unsuitable for IP.
  • Target

    • Function
      Plays fundamental roles in nuclear assembly, chromatin organization, gene expression and gonad development. May potently compress chromatin structure and be involved in membrane recruitment and chromatin decondensation during nuclear assembly. Contains 2 non-specific dsDNA-binding sites which may promote DNA cross-bridging. Exploited by retroviruses for inhibiting self-destructing autointegration of retroviral DNA, thereby promoting integration of viral DNA into the host chromosome. EMD and BAF are cooperative cofactors of HIV-1 infection. Association of EMD with the viral DNA requires the presence of BAF and viral integrase. The association of viral DNA with chromatin requires the presence of BAF and EMD.
    • Tissue specificity
      Widely expressed. Expressed in colon, brain, heart, kidney, liver, lung, ovary, pancreas, placenta, prostate, skeletal muscle, small intestine, spleen and testis. Not detected in thymus and peripheral blood leukocytes.
    • Sequence similarities
      Belongs to the BAF family.
    • Domain
      Has a helix-hairpin-helix (HhH) structural motif conserved among proteins that bind non-specifically to DNA.
      LEM domain proteins bind centrally on the BAF dimer, whereas DNA binds to the left and right sides.
    • Post-translational
      modifications
      Partially phosphorylated on serine. Ser-4 phosphorylation may block BAF ability to promote EMD binding to lamins in vitro. Non phosphorylated BAF seems to enhances binding between EMD and LMNA.
    • Cellular localization
      Nucleus. Cytoplasm. Chromosome. Significantly enriched at the nuclear inner membrane, diffusely throughout the nucleus during interphase and concentrated at the chromosomes during the M-phase. May be included in HIV-1 virions via its interaction with viral GAG polyprotein.
    • Information by UniProt
    • Database links
    • Alternative names
      • BAF antibody
      • BAF_HUMAN antibody
      • BANF 1 antibody
      • BANF1 antibody
      • Barrier to autointegration factor 1 antibody
      • Barrier to autointegration factor antibody
      • Barrier-to-autointegration factor antibody
      • BCRG 1 antibody
      • BCRG1 antibody
      • BCRP 1 antibody
      • BCRP1 antibody
      • Breakpoint cluster region protein 1 antibody
      • D14S1460 antibody
      • MGC111161 antibody
      • NGPS antibody
      see all

    Images

    • All lanes : Anti-BANF1 antibody [EPR7668] (ab129184) at 1/1000 dilution

      Lane 1 : HeLa cell lysates
      Lane 2 : HepG2 cell lysates
      Lane 3 : 293T cell lysates

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 10 kDa
    • Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized NIH/3T3 (Mouse embryo fibroblast cells)cells labeling BANF1 with ab129184 at 1/100 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/400 dilution (green). Confocal image showing mainly nuclear with cytoplasmic staining on NIH/3T3 cell line. The nuclear counterstain is DAPI (blue).

    • ab129184 staining BANF1 in MDCK cell pellets in paraffin by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Cell pellets were fixed with paraformaldehyde, permeabilized with Tween-20 and blocked with 1% serum for 2 hours at room temperature; antigen retrieval was by heat mediation in Tris/EDTA pH 9. Samples were incubated with primary antibody (1/100 in 1% BSA + 1% FBS) for 16 hours. An undiluted HRP-conjugated goat anti-rabbit IgG polyclonal was used as the secondary antibody.

      See Abreview

    • Flow cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling BANF1 (red) with ab129184 at a 1/200 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (ab172730). Blue (unlabeled control) - Cells without incubation with primary and secondary antibodies.

    • ab129184, at a 1/100 dilution, staining BANF1 in paraffin embedded Human colonic carcinoma tissue by Immunohistochemistry.
    • Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human cervix adenocarcinoma) cells labeling BANF1 with ab129184 at 1/100 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/400 dilution (green). Confocal image showing mainly nuclear with weakly cytoplasmic staining on Hela cell line. The nuclear counterstain is DAPI (blue).

    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    ab129184 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Application
    Immunocytochemistry/ Immunofluorescence
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
    Sample
    Mouse Cell (NMuMG)
    Specification
    NMuMG
    Permeabilization
    Yes - Triton 0,1%
    Fixative
    Paraformaldehyde
    Username

    Abcam user community

    Verified customer

    Submitted Jan 29 2015

    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step
    Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Tris EDTA pH 9
    Sample
    Dog Tissue sections (MDCK pellets in paraffin)
    Specification
    MDCK pellets in paraffin
    Permeabilization
    Yes - Tween-20
    Fixative
    Paraformaldehyde
    Username

    Abcam user community

    Verified customer

    Submitted Dec 03 2014

    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step
    BSA as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate pH6
    Sample
    Mouse Tissue sections (mouse pancreas tumor)
    Specification
    mouse pancreas tumor
    Permeabilization
    Yes - Tween-20
    Fixative
    Paraformaldehyde
    Username

    Abcam user community

    Verified customer

    Submitted Nov 25 2014

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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