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Products:Epigenetics and Nuclear Signaling >> Transcription >> Other factors
Overview
Product name
Anti-BANP antibody
See all BANP products (4) ...
Description
Rabbit polyclonal to BANP
Specificity
ab23762 reacts with SMAR1/BANP.
Tested applications
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse
Immunogen
Synthetic peptide derived from residues 200 - 250 of Human BANP.
Positive control
Nuclear extract from HeLa cells.
Properties
Form
Liquid
Storage instructions
Store at +4°C.
Storage buffer
Preservative: 0.09% Sodium Azide
Constituents: 8mM PBS, 60mM Citrate, 150mM Tris, pH 7 - 8
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> Transcription >> Other factors
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Western blot - BANP antibody (ab23762)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab23762 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/5000 - 1/15000.Detects a band of approximately 75 kDa (predicted molecular weight: 59 kDa).Can be blocked with BANP peptide (ab40428).
Target
Function
Controls V(D)J recombination during T-cell development by repressing T-cell receptor (TCR) beta enhancer function. Binds to scaffold/matrix attachment region beta (S/MARbeta), an ATC-rich DNA sequence located upstream of the TCR beta enhancer. Represses cyclin D1 transcription by recruiting HDAC1 to its promoter, thereby diminishing H3K9ac, H3S10ph and H4K8ac levels. Promotes TP53 'Ser-15' phosphorylation and nuclear accumulation, which causes cell cycle arrest.
Tissue specificity
Down-regulated in breast cancer cell lines.
Sequence similarities
Belongs to the BANP/SMAR1 family.
Contains 1 BEN domain.
Cellular localization
Nucleus.
Target information above from: UniProt accessionQ8N9N5
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- AAH09424 antibody
- Banp antibody
- BANP_HUMAN antibody
see all
Database links
- Entrez Gene: 54971 Human
- Entrez Gene: 53325 Mouse
- Omim: 611564 Human
- SwissProt: Q8N9N5 Human
- SwissProt: Q8VBU8 Mouse
- Unigene: 461705 Human
- Unigene: 287163 Mouse
Anti-BANP antibody images:
Western blot - BANP antibody (ab23762)
All lanes : Anti-BANP antibody (ab23762) at 0.1 µg/ml
Lane 1 : Nuclear extract of HeLa cells at 5 µg
Lane 2 : Nuclear extract of HeLa cells at 20 µg
developed using the ECL technique
Predicted band size : 59 kDa
Observed band size : 75 kDa (why is the actual band size different from the predicted?)
Exposure time : 10 minutes
References for Anti-BANP antibody (ab23762)
ab23762 has not yet been referenced specifically in any publications.
Publishing research using ab23762? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"