Overview

  • Product name
  • Description
    Rabbit polyclonal to BANP
  • Tested applications
    Suitable for: IP, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Rabbit, Horse, Guinea pig, Cow, Dog, Chimpanzee, Rhesus monkey, Chinese hamster, Orangutan
  • Immunogen

    Synthetic peptide corresponding to a region between residue 425 and the C-terminus (residue 469) of human BANP (TrEMBL entry Q96GJ7)

  • Positive control
    • Nuclear extract from HeLa cells

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: 8mM PBS, 60mM Citrate, 150mM Tris, pH 7 to 8
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab72076 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP 1/200 - 1/2500.
IHC-P 1/2000 - 1/10000.
WB 1/10000 - 1/25000. Predicted molecular weight: 59 kDa.

Target

  • Function
    Controls V(D)J recombination during T-cell development by repressing T-cell receptor (TCR) beta enhancer function. Binds to scaffold/matrix attachment region beta (S/MARbeta), an ATC-rich DNA sequence located upstream of the TCR beta enhancer. Represses cyclin D1 transcription by recruiting HDAC1 to its promoter, thereby diminishing H3K9ac, H3S10ph and H4K8ac levels. Promotes TP53 'Ser-15' phosphorylation and nuclear accumulation, which causes cell cycle arrest.
  • Tissue specificity
    Down-regulated in breast cancer cell lines.
  • Sequence similarities
    Belongs to the BANP/SMAR1 family.
    Contains 1 BEN domain.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • AAH09424 antibody
    • Banp antibody
    • BANP_HUMAN antibody
    • BEN domain-containing protein 1 antibody
    • BEND1 antibody
    • BTG3 associated nuclear protein isoform a antibody
    • Btg3-associated nuclear protein antibody
    • DKFZp761H172 antibody
    • FLJ 10177 antibody
    • FLJ 20538 antibody
    • FLJ10177 antibody
    • FLJ20538 antibody
    • Protein BANP antibody
    • Scaffold/Matrix associated region 1 antibody
    • Scaffold/matrix-associated region-1-binding protein antibody
    • SMAR 1 antibody
    • SMAR1 antibody
    • SMARBP 1 antibody
    • SMARBP1 antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung carcinoma (left) and mouse renal cell carcinoma (right) tissues labelling BANP with ab72076 at 1/5000 (0.2µg/ml). Setection: DAB.
  • All lanes : Anti-BANP antibody (ab72076) at 0.1 µg/ml

    Lane 1 : Nuclear extract from HeLa cells at 5 µg
    Lane 2 : Nuclear extract from HeLa cells at 20 µg


    Predicted band size : 59 kDa
    Observed band size : 70 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 150 kDa,250 kDa. We are unsure as to the identity of these extra bands.

References

ab72076 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab72076.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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