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Products:Cell Biology >> Cell Cycle >> Cell Cycle Inhibitors >> Other
Overview
Product name
Anti-BAP1 antibody [3c11]
See all BAP1 products (2) ...
Description
Mouse monoclonal [3c11] to BAP1
Tested applications
WB, IP, ICCmore details
Cross reactivity
Reacts with
Human
Immunogen
Recombinant full length protein (Human).
Epitope
Recognizes an epitope within the region of amino acids 337-440 of the human BAP1 molecule.
Positive control
Whole cell extract of human melanoma cell line.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 15mM Sodium Azide
Concentration
Concentration information loading...
Purity
Ascites
Clonality
Monoclonal
Clone number
3c11
Isotype
IgG1
Research areas
Epigenetics and Nuclear Signaling >> Ubiquitin & Ubiquitin Like Modifiers >> Deubiquitination
Cancer >> Cell cycle >> Cell cycle inhibitors >> Other
Epigenetics and Nuclear Signaling >> Cell cycle >> Cell Cycle Inhibitors >> Other
Cell Biology >> Cell Cycle >> Cell Cycle Inhibitors >> Other
Applications
Show applications key
Our Abpromise guarantee covers the use of ab10531 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/2000Detects a band of approximately 81 kDa (predicted molecular weight: 81 kDa).
IP: Use at an assay dependent dilution.
ICC: Use at an assay dependent dilution.
Target
Function
Deubiquitinating enzyme that plays a key role in chromatin by mediating deubiquitination of histone H2A and HCFC1. Catalytic component of the PR-DUB complex, a complex that specifically mediates deubiquitination of histone H2A monoubiquitinated at 'Lys-119' (H2AK119ub1). Does not deubiquitinate monoubiquitinated histone H2B. Acts as a regulator of cell growth by mediating deubiquitination of HCFC1 N-terminal and C-terminal chains, with some specificity toward 'Lys-48'-linked polyubiquitin chains compared to 'Lys-63'-linked polyubiquitin chains. Deubiquitination of HCFC1 does not lead to increase stability of HCFC1. Interferes with the BRCA1 and BARD1 heterodimer activity by inhibiting their ability to mediate ubiquitination and autoubiquitination. It however does not mediate deubiquitination of BRCA1 and BARD1. Acts as a tumor suppressor.
Tissue specificity
Highly expressed in testis, placenta and ovary. Expressed in breast.
Sequence similarities
Belongs to the peptidase C12 family. BAP1 subfamily.
Post-translational
modifications
Phosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localization
Cytoplasm. Nucleus. Mainly nuclear. Binds to chromatin.
Target information above from: UniProt accessionQ92560
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- BAP 1 antibody
- Bap1 antibody
- BAP1_HUMAN antibody
see all
Database links
- Entrez Gene: 8314 Human
- Omim: 603089 Human
- SwissProt: Q92560 Human
- Unigene: 106674 Human
References for Anti-BAP1 antibody [3c11] (ab10531)
ab10531 has not yet been referenced specifically in any publications.
Publishing research using ab10531? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"