Anti-BAP31 antibody [7A3BB6] (ab112993)

  Western blot - BAP31 antibody [7A3BB6] (ab112993)

All lanes : Anti-BAP31 antibody [7A3BB6] (ab112993) at 0.5 µg/ml

Lane 1 : HepG2 Cell Lysate
Lane 2 : H9C2 Cell Lysate
Lane 3 : MEF Cell Lysate


Predicted band size : 28 kDa

  Flow Cytometry - BAP31 antibody [7A3BB6] (ab112993)

ab112993 at 1ug/ml staining BAP31 in MeOH-fixed HL60 cells by Flow Cytometry (blue). Isotype control antibody (red).

  Immunocytochemistry/ Immunofluorescence - Anti-BAP31 antibody [7A3BB6] (ab112993)

ab112993 at 5 µg/ml staining BAP31 in Human fibroblast cells by Immunocytochemistry (4% paraformaldehyde fixed and 0.1% Triton X-100 permeabilized) followed by Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1 hour (green). The target locates mainly in the endoplasmic reticulum.

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BAP31 antibody [7A3BB6] (ab112993)

IHC image of BAP31 staining in Human normal colon formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab112993, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.