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Anti-BARD1 antibody [2059C4a] (ab50984) at 1/50 dilution + HEK293 whole cell lysate at 50 µg
Secondary
Anti mouse IgG antibody at 1/2500 dilution
Predicted band size : 86 kDa
Observed band size : 100 kDa (why is the actual band size different from the predicted?)
Ab50984 at 1/50 dilution precipitating RIPA lysate of HeLa cells at 500µg; visualised with anti mouse IgG antibody at 1/2500 diltuion.
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