![Western blot - BARD1 antibody [2059C4a] (ab50984) image](#ab50984wb.jpg)
![Immunoprecipitation - BARD1 antibody [2059C4a] (ab50984) image](#ab50984ip.jpg)
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Products:Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> DNA Damage Response >> DNA Damage Recognition
Overview
Product name
Anti-BARD1 antibody [2059C4a]
See all BARD1 products (4) ...
Description
Mouse monoclonal [2059C4a] to BARD1
Tested applications
Dot Blot, WB, IPmore details
Cross reactivity
Reacts with
Human
Immunogen
Recombinant fragment (Human)
Positive control
HEK293 whole cell lysate, RIPA lysate of HeLa cells.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.05% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Protein G purified
Purification notes
Protein G purified from culture of supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (~95%) fetal bovine serum. Filtered through a 0.22 µm membrane.
Clonality
Monoclonal
Clone number
2059C4a
Isotype
IgG1
Research areas
Cancer >> Oncoproteins/suppressors >> Tumor suppressors >> Other
Epigenetics and Nuclear Signaling >> Cell cycle >> Apoptosis >> Nuclear
Epigenetics and Nuclear Signaling >> Transcription >> Cancer susceptibility >> Tumor Suppressors
Cell Biology >> Proteolysis / Ubiquitin >> Proteasome / Ubiquitin >> Ubiquitin E2s and E3s >> RING Finger E3 Ligase
Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> DNA Damage Response >> DNA Damage Recognition
Applications
Show applications key
Our Abpromise guarantee covers the use of ab50984 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Dot: Use at an assay dependent dilution.
WB: 1/50Predicted molecular weight: 86 kDa.
IP: 1/50
Target
Function
Probable E3 ubiquitin-protein ligase. The BRCA1-BARD1 heterodimer specifically mediates the formation of 'Lys-6'-linked polyubiquitin chains and coordinates a diverse range of cellular pathways such as DNA damage repair, ubiquitination and transcriptional regulation to maintain genomic stability. Plays a central role in the control of the cell cycle in response to DNA damage. Acts by mediating ubiquitin E3 ligase activity that is required for its tumor suppressor function. Also forms a heterodimer with CSTF1/CSTF-50 to modulate mRNA processing and RNAP II stability by inhibiting pre-mRNA 3' cleavage.
Pathway
Protein modification; protein ubiquitination.
Sequence similarities
Contains 4 ANK repeats.
Contains 2 BRCT domains.
Contains 1 RING-type zinc finger.
Post-translational
modifications
Processed during apoptosis. The homodimer is more susceptible to proteolytic cleavage than the BARD1/BRCA1 heterodimer.
Cellular localization
Nucleus. During S phase of the cell cycle, colocalizes with BRCA1 into discrete subnuclear foci. Can translocate to the cytoplasm. Localizes at sites of DNA damage at double-strand breaks (DSBs); recruitment to DNA damage sites is mediated by the BRCA1-A complex.
Target information above from: UniProt accessionQ99728
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- BARD-1 antibody
- Bard1 antibody
- BARD1_HUMAN antibody
see all
Database links
- Entrez Gene: 580 Human
- NCBI: 4557349 Human
- Omim: 601593 Human
- SwissProt: Q99728 Human
- Unigene: 591642 Human
Anti-BARD1 antibody [2059C4a] images:
Western blot - BARD1 antibody [2059C4a] (ab50984)
![Western blot - BARD1 antibody [2059C4a] (ab50984)](/ps/datasheet/Images/50/ab50984/ab50984wb.jpg)
Anti-BARD1 antibody [2059C4a] (ab50984) at 1/50 dilution + HEK293 whole cell lysate at 50 µg
Secondary
Anti mouse IgG antibody at 1/2500 dilution
Predicted band size : 86 kDa
Observed band size : 100 kDa (why is the actual band size different from the predicted?)
Immunoprecipitation - BARD1 antibody [2059C4a] (ab50984)
![Immunoprecipitation - BARD1 antibody [2059C4a] (ab50984)](/ps/datasheet/Images/50/ab50984/ab50984ip.jpg)
Ab50984 at 1/50 dilution precipitating RIPA lysate of HeLa cells at 500µg; visualised with anti mouse IgG antibody at 1/2500 diltuion.
References for Anti-BARD1 antibody [2059C4a] (ab50984)
ab50984 has not yet been referenced specifically in any publications.
Publishing research using ab50984? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"