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Reassurance, Refunds & Replacements
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »This product is covered by the Abpromise guarantee. Our scientific support team are available to answer any questions or queries - fill out an inquiry form for ab64164 for help.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Western blot - BARD1 antibody (ab64164)
Anti-BARD1 antibody (ab64164) at 2 µg/ml + HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 20 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size : 87 kDa
Observed band size : 95 kDa (why is the actual band size different from the predicted?)
Additional bands at : 23 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 12 minutes
The 95 kDa band observed is comparable to the molecular weight seen with other commercially available antibodies to BARD1.
Immunocytochemistry/ Immunofluorescence - Anti-BARD1 antibody (ab64164)
ICC/IF image of ab64164 stained MCF7 cells. The cells were 4% formaldehyde (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab64164, 10µg/ml) overnight at +4ºC. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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