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Products:Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> DNA Damage Response >> DNA Damage Recognition
Overview
Product name
Anti-BARD1 antibody
See all BARD1 products (4) ...
Description
Rabbit polyclonal to BARD1
Tested applications
ICC/IF, WBmore details
Cross reactivity
Reacts with
Human
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human BARD1.
(Peptide available as ab74356.)
Positive control
This antibody gave a positive signal in HeLa Whole Cell Lysate.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Oncoproteins/suppressors >> Tumor suppressors >> Other
Epigenetics and Nuclear Signaling >> Cell cycle >> Apoptosis >> Nuclear
Epigenetics and Nuclear Signaling >> Transcription >> Cancer susceptibility >> Tumor Suppressors
Cell Biology >> Proteolysis / Ubiquitin >> Proteasome / Ubiquitin >> Ubiquitin E2s and E3s >> RING Finger E3 Ligase
Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> DNA Damage Response >> DNA Damage Recognition
Applications
Show applications key
Our Abpromise guarantee covers the use of ab64164 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: Use a concentration of 10 µg/ml
WB: Use a concentration of 2 µg/mlDetects a band of approximately 95 kDa (predicted molecular weight: 87 kDa).
Target
Function
Probable E3 ubiquitin-protein ligase. The BRCA1-BARD1 heterodimer specifically mediates the formation of 'Lys-6'-linked polyubiquitin chains and coordinates a diverse range of cellular pathways such as DNA damage repair, ubiquitination and transcriptional regulation to maintain genomic stability. Plays a central role in the control of the cell cycle in response to DNA damage. Acts by mediating ubiquitin E3 ligase activity that is required for its tumor suppressor function. Also forms a heterodimer with CSTF1/CSTF-50 to modulate mRNA processing and RNAP II stability by inhibiting pre-mRNA 3' cleavage.
Pathway
Protein modification; protein ubiquitination.
Sequence similarities
Contains 4 ANK repeats.
Contains 2 BRCT domains.
Contains 1 RING-type zinc finger.
Post-translational
modifications
Processed during apoptosis. The homodimer is more susceptible to proteolytic cleavage than the BARD1/BRCA1 heterodimer.
Cellular localization
Nucleus. During S phase of the cell cycle, colocalizes with BRCA1 into discrete subnuclear foci. Can translocate to the cytoplasm. Localizes at sites of DNA damage at double-strand breaks (DSBs); recruitment to DNA damage sites is mediated by the BRCA1-A complex.
Target information above from: UniProt accessionQ99728
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- BARD-1 antibody
- Bard1 antibody
- BARD1_HUMAN antibody
see all
Database links
- Entrez Gene: 580 Human
- NCBI: 4557349 Human
- Omim: 601593 Human
- SwissProt: Q99728 Human
- Unigene: 591642 Human
Anti-BARD1 antibody images:
Western blot - BARD1 antibody (ab64164)
Anti-BARD1 antibody (ab64164) at 2 µg/ml + HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 20 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size : 87 kDa
Observed band size : 95 kDa (why is the actual band size different from the predicted?)
Additional bands at : 23 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 12 minutes
The 95 kDa band observed is comparable to the molecular weight seen with other commercially available antibodies to BARD1.
Immunocytochemistry/ Immunofluorescence - Anti-BARD1 antibody (ab64164)
ICC/IF image of ab64164 stained MCF7 cells. The cells were 4% formaldehyde (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab64164, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
References for Anti-BARD1 antibody (ab64164)
This product has been referenced in:
- Banaszynski LAet al. Analysis of histones and chromatin in Xenopus laevis egg and oocyte extracts. Methods 51:3-10 (2010). WB.Read more (PubMed: 20051265) »
See 1 publication for this product
Publishing research using ab64164? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"