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Products:Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> DNA Damage Response >> DNA Damage Recognition
Overview
Product name
Anti-BARD1 antibody
See all BARD1 products (4) ...
Description
Rabbit polyclonal to BARD1
Specificity
ab69645 detects endogenous levels of total BARD1 protein.
Tested applications
ELISA, WBmore details
Cross reactivity
Reacts with
Human
Immunogen
Synthesized peptide derived from N-terminal of human BARD1.
Positive control
HeLa cell extracts.
Properties
Form
Liquid
Storage instructions
Store at -20°C. Stable for 12 months at -20°C
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
ab69645 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Oncoproteins/suppressors >> Tumor suppressors >> Other
Epigenetics and Nuclear Signaling >> Cell cycle >> Apoptosis >> Nuclear
Epigenetics and Nuclear Signaling >> Transcription >> Cancer susceptibility >> Tumor Suppressors
Cell Biology >> Proteolysis / Ubiquitin >> Proteasome / Ubiquitin >> Ubiquitin E2s and E3s >> RING Finger E3 Ligase
Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> DNA Damage Response >> DNA Damage Recognition
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Western blot - BARD1 antibody (ab69645)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab69645 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA
ELISA: 1/10000ELISA: 1/10000
WB: 1/500 - 1/1000.Detects a band of approximately 80 kDa (predicted molecular weight: 86 kDa).
Target
Function
Probable E3 ubiquitin-protein ligase. The BRCA1-BARD1 heterodimer specifically mediates the formation of 'Lys-6'-linked polyubiquitin chains and coordinates a diverse range of cellular pathways such as DNA damage repair, ubiquitination and transcriptional regulation to maintain genomic stability. Plays a central role in the control of the cell cycle in response to DNA damage. Acts by mediating ubiquitin E3 ligase activity that is required for its tumor suppressor function. Also forms a heterodimer with CSTF1/CSTF-50 to modulate mRNA processing and RNAP II stability by inhibiting pre-mRNA 3' cleavage.
Pathway
Protein modification; protein ubiquitination.
Sequence similarities
Contains 4 ANK repeats.
Contains 2 BRCT domains.
Contains 1 RING-type zinc finger.
Post-translational
modifications
Processed during apoptosis. The homodimer is more susceptible to proteolytic cleavage than the BARD1/BRCA1 heterodimer.
Cellular localization
Nucleus. During S phase of the cell cycle, colocalizes with BRCA1 into discrete subnuclear foci. Can translocate to the cytoplasm. Localizes at sites of DNA damage at double-strand breaks (DSBs); recruitment to DNA damage sites is mediated by the BRCA1-A complex.
Target information above from: UniProt accessionQ99728
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- BARD-1 antibody
- Bard1 antibody
- BARD1_HUMAN antibody
see all
Database links
- Entrez Gene: 580 Human
- NCBI: 4557349 Human
- Omim: 601593 Human
- SwissProt: Q99728 Human
- Unigene: 591642 Human
Anti-BARD1 antibody images:
Western blot - BARD1 antibody (ab69645)
All lanes : Anti-BARD1 antibody (ab69645) at 1/500 dilution
Lane 1 : HeLa cell extract
Lane 2 : HeLa cell extract with immunising peptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size : 86 kDa
Observed band size : 80 kDa (why is the actual band size different from the predicted?)
Additional bands at : 60 kDa. We are unsure as to the identity of these extra bands.
References for Anti-BARD1 antibody (ab69645)
ab69645 has not yet been referenced specifically in any publications.
Publishing research using ab69645? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"