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Western blot - BARD1 antibody (ab69645)
(enlarge)
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Product Name
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BARD1 antibody
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See all BARD1 antibodies (4)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to BARD1
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Immunogen
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Synthesized peptide derived from N-terminal of human BARD1.
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Reacts with
(species key)
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Hu
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Specificity
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ab69645 detects endogenous levels of total BARD1 protein.
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Tested applications
(see key)
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ELISA, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ELISA: 1/10000.
WB: 1/500 - 1/1000. Detects a band of approximately 80 kDa (predicted molecular weight: 86 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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HeLa cell extracts.
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Cellular localization
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Nuclear. During S phase of the cell cycle, colocalizes with BRCA1 into discrete subnuclear foci. Can translocate to the cytoplasm.
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Research areas
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Cancer >> Oncoproteins/suppressors >> Tumor suppressors >> Other
Chromatin and Nuclear Signaling >> Cell cycle >> Apoptosis >> Nuclear
Chromatin and Nuclear Signaling >> Transcription >> Cancer susceptibility >> Tumor Suppressors
Cell Biology >> Proteolysis / Ubiquitin >> Proteasome / Ubiquitin >> Ubiquitin E2s and E3s >> RING Finger E3 Ligase
Chromatin and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> DNA Damage Response >> DNA Damage Recognition
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Relevance
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BRCA1 Associated RING Domain gene 1 (BARD1) interacts with the N terminal region of BRCA1. In addition to its ability to bind BRCA1 in vivo and in vitro, BARD1 shares homology with the 2 most conserved regions of BRCA1: the N terminal RING motif and the C terminal BRCT domain. The RING motif is a cysteine rich sequence found in a variety of proteins that regulate cell growth, including the products of tumor suppressor genes and dominant protooncogenes. The BARD1 protein also contains 3 tandem ankyrin repeats. The BARD1/BRCA1 interaction is disrupted by tumorigenic amino acid substitutions in BRCA1, implying that the formation of a stable complex between these proteins may be an essential aspect of BRCA1 tumor suppression. BARD1 may be the target of oncogenic mutations in breast or ovarian cancer. BARD1 also plays a role in mediating apoptotic stress and p53 dependent apoptosis.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4 Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS
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Purification notes
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ab69645 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Store at -20°C. Stable for 12 months at -20°C
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this BARD1 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for BARD1 antibody images, references, products related to ab69645 and other tools.
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BARD1 antibody images:
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 Western blot - BARD1 antibody (ab69645)
All lanes : BARD1 antibody (ab69645) at 1/500 dilution
Lane 1 : HeLa cell extract
Lane 2 : HeLa cell extract with immunising peptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size : 86 kDa
Observed band size : 80 kDa (why is the actual band size different from the predicted?)
Additional bands at : 60 kDa. We are unsure as to the identity of these extra bands.
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Search PubMed (MEDLINE) for references to BARD1
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BARD1 antibody - more information
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Customer reviews (feedback) regarding BARD1 antibody |
Customer FAQs regarding BARD1 antibody |
Protocols for BARD1 antibody |
Price and availability of products related to BARD1 antibody |
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BARD1 antibody - related products:
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BARD1 antibody - other tools:
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Contact Abcam with a Technical Enquiry about BARD1 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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