Anti-BAT3/BAG-6 antibody (ab88292)
Key features and details
- Mouse polyclonal to BAT3/BAG-6
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
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Overview
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Product name
Anti-BAT3/BAG-6 antibody
See all BAT3/BAG-6 primary antibodies -
Description
Mouse polyclonal to BAT3/BAG-6 -
Host species
Mouse -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein within Human BAT3/BAG-6. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: NP_542433.1 -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.40
Constituent: 100% PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab88292 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 1 µg/ml. Predicted molecular weight: 119 kDa.
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ICC/IF |
Use a concentration of 10 µg/ml.
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Notes |
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WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 119 kDa. |
ICC/IF
Use a concentration of 10 µg/ml. |
Target
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Function
Chaperone that plays a key role in various processes such as apoptosis, insertion of tail-anchored (TA) membrane proteins to the endoplasmic reticulum membrane and regulation of chromatin. Acts in part by regulating stability of proteins and their degradation by the proteasome. Participates in endoplasmic reticulum stress-induced apoptosis via its interaction with AIFM1/AIF by regulating AIFM1/AIF stability and preventing its degradation. Also required during spermatogenesis for synaptonemal complex assembly via its interaction with HSPA2, by inhibiting polyubiquitination and subsequent proteasomal degradation of HSPA2. Required for selective ubiquitin-mediated degradation of defective nascent chain polypeptides by the proteasome. In this context, may play a role in immuno-proteasomes to generate antigenic peptides via targeted degradation, thereby playing a role in antigen presentation in immune response. Key component of the BAG6/BAT3 complex, a cytosolic multiprotein complex involved in the post-translational delivery of tail-anchored (TA) membrane proteins to the endoplasmic reticulum membrane. TA membrane proteins, also named type II transmembrane proteins, contain a single C-terminal transmembrane region. BAG6/BAT3 acts by facilitating TA membrane proteins capture by ASNA1/TRC40: it is recruited to ribosomes synthesizing membrane proteins, interacts with the transmembrane region of newly released TA proteins and transfers them to ASNA1/TRC40 for targeting to the endoplasmic reticulum membrane. Also involved in DNA damage-induced apoptosis: following DNA damage, accumulates in the nucleus and forms a complex with p300/EP300, enhancing p300/EP300-mediated p53/TP53 acetylation leading to increase p53/TP53 transcriptional activity. When nuclear, may also act as a component of some chromatin regulator complex that regulates histone 3 'Lys-4' dimethylation (H3K4me2). -
Sequence similarities
Contains 1 ubiquitin-like domain. -
Post-translational
modificationsCleavage by caspase-3 releases a C-terminal peptide that plays a role in ricin-induced apoptosis.
In case of infection by L.pneumophila, ubiquitinated by the SCF(LegU1) complex. -
Cellular localization
Cytoplasm > cytosol. Nucleus. The C-terminal fragment generated by caspase-3 is cytoplasmic. - Information by UniProt
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Database links
- Entrez Gene: 7917 Human
- Omim: 142590 Human
- SwissProt: P46379 Human
- Unigene: 440900 Human
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Alternative names
- 2410045D21Rik antibody
- AA408914 antibody
- BAG 6 antibody
see all
Images
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Anti-BAT3/BAG-6 antibody (ab88292) at 1 µg/ml + A-431 cell lysate with BAT3 / BAG-6 expression at 50 µg
Secondary
Goat Anti-Mouse IgG at 1/2500 dilution
Predicted band size: 119 kDa
Observed band size: 150 kDa why is the actual band size different from the predicted? -
All lanes : Anti-BAT3/BAG-6 antibody (ab88292) at 1 µg/ml
Lane 1 : BAT3/BAG-6 transfected 293T cell lysate
Lane 2 : Non-transfected 293T cell lysate
Lysates/proteins at 50 µg per lane.
Secondary
All lanes : Goat Anti-Mouse IgG at 1/2500 dilution
Predicted band size: 119 kDa
Observed band size: 150 kDa why is the actual band size different from the predicted? -
Immunofluorescence of ab88292 on HeLa cell with antibody concentration at 10 ug/ml
Protocols
Datasheets and documents
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Datasheet download
References (3)
ab88292 has been referenced in 3 publications.
- Panner Selvam MK et al. Treatment of semen samples with a-chymotrypsin alters the expression pattern of sperm functional proteins-a pilot study. Andrology 6:345-350 (2018). PubMed: 29409138
- Preta G & Fadeel B Scythe cleavage during Fas (APO-1)-and staurosporine-mediated apoptosis. FEBS Lett 586:747-52 (2012). PubMed: 22285488
- Claessen JH & Ploegh HL BAT3 Guides Misfolded Glycoproteins Out of the Endoplasmic Reticulum. PLoS One 6:e28542 (2011). WB, ICC/IF ; Human . PubMed: 22174835