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Products:Immunology >> Adaptive Immunity >> MHC >> Class II
Overview
Product name
Anti-BAT4 antibody
See all BAT4 products (2) ...
Description
Mouse polyclonal to BAT4
Tested applications
Cross reactivity
Reacts with
Human
Immunogen
Full length human BAT4 protein (NP_149417, 1 a.a. ~ 356 a.a)
Positive control
BAT4 transfected 293T cell lysate.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: Whole serum
Purity
Whole antiserum
Clonality
Polyclonal
Isotype
IgG
Research areas
Immunology >> Adaptive Immunity >> MHC >> Class II
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Western blot - BAT4 antibody (ab72716)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab72716 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/500 - 1/1000.Detects a band of approximately 35 kDa (predicted molecular weight: 39 kDa).
Target
Relevance
BAT4 (HLA-B-associated transcript 4) contains two ANK repeats and one G-patch domain. It is thought to be involved in some aspects of immunity.
Cellular localization
Endoplasmic reticulum
Alternative names
- ANKRD59 antibody
- Ankyrin repeat domain containing protein 59 antibody
- BAT 4 antibody
see all
Database links
- Entrez Gene: 7918 Human
- Omim: 142610 Human
- SwissProt: O95872 Human
Anti-BAT4 antibody images:
Western blot - BAT4 antibody (ab72716)
All lanes : Anti-BAT4 antibody (ab72716) at 1/500 dilution
Lane 1 : BAT4 transfected 293T cell lysate
Lane 2 : Non-transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Secondary
Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
Predicted band size : 39 kDa
Observed band size : 35 kDa (why is the actual band size different from the predicted?)
References for Anti-BAT4 antibody (ab72716)
This product has been referenced in:
- Ramirez ABet al. Use of a single-chain antibody library for ovarian cancer biomarker discovery. Mol Cell Proteomics 9:1449-60 (2010).Read more (PubMed: 20467042) »
See 1 publication for this product
Publishing research using ab72716? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"