Recombinant Anti-BAT5 antibody [EPR15463] - C-terminal (ab185549)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR15463] to BAT5 - C-terminal
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-BAT5 antibody [EPR15463] - C-terminal
See all BAT5 primary antibodies -
Description
Rabbit monoclonal [EPR15463] to BAT5 - C-terminal -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Caco-2, 293, HT29 and BxPC-3 cell lysate. BxPC-3 cell extract.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS, 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR15463 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab185549 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/2000. Detects a band of approximately 55 kDa (predicted molecular weight: 63 kDa).
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Notes |
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WB
1/1000 - 1/2000. Detects a band of approximately 55 kDa (predicted molecular weight: 63 kDa). |
Target
- Information by UniProt
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Database links
- Entrez Gene: 7920 Human
- Entrez Gene: 193742 Mouse
- Entrez Gene: 361796 Rat
- Omim: 142620 Human
- SwissProt: O95870 Human
- SwissProt: Q9Z1Q2 Mouse
- SwissProt: Q6MG55 Rat
- Unigene: 388188 Human
see all -
Alternative names
- ABHD16A antibody
- ABHGA_HUMAN antibody
- Abhydrolase domain containing 16A antibody
see all
Images
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All lanes : Anti-BAT5 antibody [EPR15463] - C-terminal (ab185549) at 1/1000 dilution
Lane 1 : Caco-2 cell lysate
Lane 2 : 293 cell lysate
Lane 3 : BxPC-3 cell lysate
Lane 4 : HT-29 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 63 kDa
Observed band size: 55 kDa why is the actual band size different from the predicted? -
All lanes : Anti-BAT5 antibody [EPR15463] - C-terminal (ab185549) at 1/1000 dilution
Lane 1 : BxPC-3 cell extract
Lane 2 : BxPC-3 cell extract with immunizing peptide
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 63 kDa
Observed band size: 55 kDa why is the actual band size different from the predicted?
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (3)
ab185549 has been referenced in 3 publications.
- Yang L et al. Mirtronic miR-4646-5p promotes gastric cancer metastasis by regulating ABHD16A and metabolite lysophosphatidylserines. Cell Death Differ 28:2708-2727 (2021). PubMed: 33875796
- Singh S et al. Mapping the Neuroanatomy of ABHD16A, ABHD12, and Lysophosphatidylserines Provides New Insights into the Pathophysiology of the Human Neurological Disorder PHARC. Biochemistry 59:2299-2311 (2020). PubMed: 32462874
- Yokoi N et al. Identification of PSD-95 Depalmitoylating Enzymes. J Neurosci 36:6431-44 (2016). PubMed: 27307232