Recombinant Anti-BBS1 antibody [EPR7906] (ab166613)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7906] to BBS1
- Suitable for: WB, ICC/IF
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-BBS1 antibody [EPR7906]
See all BBS1 primary antibodies -
Description
Rabbit monoclonal [EPR7906] to BBS1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details
Unsuitable for: Flow Cyt,IHC-P or IP -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide within Human BBS1. The exact sequence is proprietary.
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Positive control
- SH-SY5Y, fetal brain, fetal kidney, and 293T lysates, SH-SY5Y cells
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.2
Constituents: 0.35% Sodium citrate, 0.17% Sodium chloride, 0.03% EDTA, 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR7906 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab166613 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/5000. Detects a band of approximately 65 kDa (predicted molecular weight: 65 kDa).
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ICC/IF |
1/100 - 1/250.
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Notes |
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WB
1/1000 - 1/5000. Detects a band of approximately 65 kDa (predicted molecular weight: 65 kDa). |
ICC/IF
1/100 - 1/250. |
Target
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Function
The BBSome complex is required for ciliogenesis but is dispensable for centriolar satellite function. This ciliogenic function is mediated in part by the Rab8 GDP/GTP exchange factor, which localizes to the basal body and contacts the BBSome. Rab8(GTP) enters the primary cilium and promotes extension of the ciliary membrane. Firstly the BBSome associates with the ciliary membrane and binds to Rabin8, the guanosyl exchange factor (GEF) for Rab8 and then the Rab8-GTP localizes to the cilium and promotes docking and fusion of carrier vesicles to the base of the ciliary membrane. -
Tissue specificity
Highly expressed in the kidney. Also found in fetal tissue, testis, retina, adipose tissue, heart, skeletal muscle and pancreas. -
Involvement in disease
Defects in BBS1 are a cause of Bardet-Biedl syndrome type 1 (BBS1) [MIM:209900]. Bardet-Biedl syndrome (BBS) is a genetically heterogeneous disorder characterized by usually severe pigmentary retinopathy, early onset obesity, polydactyly, hypogenitalism, renal malformation and mental retardation. Secondary features include diabetes mellitus, hypertension and congenital heart disease. A relatively high incidence of BBS is found in the mixed Arab populations of Kuwait and in Bedouin tribes throughout the Middle East, most likely due to the high rate of consaguinity in these populations and a founder effect. Inheritance is autosomal recessive, but three mutated alleles (two at one locus, and a third at a second locus) may be required for disease manifestation in some cases (triallelic inheritance). -
Cellular localization
Cell projection > cilium membrane. Cytoplasm. Localizes to nonmembranous centriolar satellites in the cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 582 Human
- Entrez Gene: 52028 Mouse
- Entrez Gene: 309156 Rat
- Omim: 209901 Human
- SwissProt: Q8NFJ9 Human
- Unigene: 502915 Human
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Alternative names
- AI451249 antibody
- Bardet-Biedl syndrome 1 antibody
- Bardet-Biedl syndrome 1 homolog antibody
see all
Images
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All lanes : Anti-BBS1 antibody [EPR7906] (ab166613) at 1/1000 dilution
Lane 1 : SH-SY5Y lysate
Lane 2 : fetal brain lysate
Lane 3 : fetal kidney lysate
Lane 4 : 293T lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 65 kDa
Observed band size: 65 kDa -
Immunofluorescent analysis of SH-SY5Y cells labeling BBS1 with ab166613 at 1/100.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab166613 has not yet been referenced specifically in any publications.