Anti-BBS4 antibody (ab197122)
Key features and details
- Rabbit polyclonal to BBS4
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-BBS4 antibody -
Description
Rabbit polyclonal to BBS4 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Cow -
Immunogen
Recombinant fragment corresponding to Human BBS4 aa 300 to the C-terminus. (BC027624)
Database link: Q96RK4 -
Positive control
- HeLa and 293 cell lysates; Human fetal pancreas tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Lyophilized:Reconstitute in 200ul sterile H2O. -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 98% PBS, 1% BSA -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab197122 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/100 - 1/500.
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WB |
1/200 - 1/1000. Predicted molecular weight: 58 kDa.
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Notes |
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IHC-P
1/100 - 1/500. |
WB
1/200 - 1/1000. Predicted molecular weight: 58 kDa. |
Target
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Function
May be required for the dynein-mediated transport of pericentriolar proteins to the centrosome. Required for microtubule anchoring at the centrosome but not for microtubule nucleation. The BBSome complex is required for ciliogenesis but is dispensable for centriolar satellite function. This ciliogenic function is mediated in part by the Rab8 GDP/GTP exchange factor, which localizes to the basal body and contacts the BBSome. Rab8(GTP) enters the primary cilium and promotes extension of the ciliary membrane. Firstly the BBSome associates with the ciliary membrane and binds to Rabin8, the guanosyl exchange factor (GEF) for Rab8 and then the Rab8-GTP localizes to the cilium and promotes docking and fusion of carrier vesicles to the base of the ciliary membrane. -
Tissue specificity
Ubiquitously expressed. The highest level of expression is found in the kidney. -
Involvement in disease
Defects in BBS4 are the cause of Bardet-Biedl syndrome type 4 (BBS4) [MIM:209900]. Bardet-Biedl syndrome (BBS) is a genetically heterogeneous, autosomal recessive disorder characterized by usually severe pigmentary retinopathy, early onset obesity, polydactyly, hypogenitalism, renal malformation and mental retardation. Secondary features include diabetes mellitus, hypertension and congenital heart disease. A relatively high incidence of BBS is found in the mixed Arab populations of Kuwait and in Bedouin tribes throughout the Middle East, most likely due to the high rate of consaguinity in these populations and a founder effect. -
Sequence similarities
Belongs to the BBS4 family.
Contains 10 TPR repeats. -
Cellular localization
Cytoplasm > cytoskeleton > centrosome. Cytoplasm > cytoskeleton. Cell projection > cilium membrane. Cytoplasm. Localizes to the pericentriolar region throughout the cell cycle. Centrosomal localization requires dynein. Localizes to nonmembranous centriolar satellites in the cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 532120 Cow
- Entrez Gene: 585 Human
- Entrez Gene: 102774 Mouse
- Omim: 600374 Human
- SwissProt: Q1JQ97 Cow
- SwissProt: Q96RK4 Human
- SwissProt: Q8C1Z7 Mouse
- Unigene: 208681 Human
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Alternative names
- Bardet Biedl syndrome 4 protein antibody
- Bardet-Biedl syndrome 4 protein antibody
- Bbs4 antibody
- BBS4_HUMAN antibody
Images
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab197122 has been referenced in 2 publications.
- Chao YY et al. ATM- and ATR-induced primary ciliogenesis promotes cisplatin resistance in pancreatic ductal adenocarcinoma. J Cell Physiol 237:4487-4503 (2022). PubMed: 36251015
- Kyun ML et al. Wnt3a Stimulation Promotes Primary Ciliogenesis through ß-Catenin Phosphorylation-Induced Reorganization of Centriolar Satellites. Cell Rep 30:1447-1462.e5 (2020). PubMed: 32023461