![Western blot - BBX antibody [2065C12a] (ab50824) image](#bbx.jpg)
![Immunofluorescence - BBX antibody [2065C12a] (ab50824) image](#bbxif.jpg)
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Products:Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> HMG Box
Overview
Product name
Anti-BBX antibody [2065C12a]
See all BBX products (3) ...
Description
Mouse monoclonal [2065C12a] to BBX
Tested applications
WB, ICC/IFmore details
Cross reactivity
Reacts with
Human
Immunogen
Recombinant fragment (Human)
Positive control
HeLa cells or 3T3 cells nuclear or whole celle lysate
Properties
Form
Liquid
Storage instructions
Store at 4°C for up to one year. Upon use, aliquot and store at -20°C or -80°C.
Storage buffer
Preservative: 0.05% Sodium Azide
Constituents: 1% BSA, PBS
Concentration
Concentration information loading...
Purity
Protein G purified
Purification notes
ab50824 was purified from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum. Filter sterilized.
Clonality
Monoclonal
Clone number
2065C12a
Isotype
IgG1
Research areas
Epigenetics and Nuclear Signaling >> Transcription >> Transcription Factors
Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> HMG Box
Applications
Show applications key
Our Abpromise guarantee covers the use of ab50824 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/50Predicted molecular weight: 105 kDa.
ICC/IF
ICC/IF: 1/50ICC/IF: 1/50
Target
Relevance
BBX is a new mammalian protein that complements the fission yeast MBF transcription complex
Cellular localization
Nuclear
Alternative names
- Bobby sox homolog antibody
- HMG box containing protein 2 antibody
- HBP2 antibody
see all
Database links
- Entrez Gene: 56987 Human
- SwissProt: A2RRM7 Human
- SwissProt: Q8WY36 Human
Anti-BBX antibody [2065C12a] images:
Western blot - BBX antibody [2065C12a] (ab50824)
![Western blot - BBX antibody [2065C12a] (ab50824)](/ps/datasheet/Images/50/ab50824/bbx.jpg)
Anti-BBX antibody [2065C12a] (ab50824) at 1/50 dilution + HeLa whole cell lysate at 50 µg
Secondary
anti-mouse IgG at 1/2500 dilution
Predicted band size : 105 kDa
Observed band size : 130 kDa (why is the actual band size different from the predicted?)
Immunofluorescence - BBX antibody [2065C12a] (ab50824)
![Immunofluorescence - BBX antibody [2065C12a] (ab50824)](/ps/datasheet/Images/50/ab50824/bbxif.jpg)
Immunofluorescent staining of BBX expression HeLa cells using ab50824 at a 1/50 dilution and and AlexaFluor 488 conjugated goat anti-mouse secondary
References for Anti-BBX antibody [2065C12a] (ab50824)
ab50824 has not yet been referenced specifically in any publications.
Publishing research using ab50824? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"