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Western blot - BBX antibody [2065C12a] (ab50824)
![Western blot - BBX antibody [2065C12a] (ab50824)](/ps/datasheet/images/bbx.jpg)
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Immunofluorescence - BBX antibody [2065C12a] (ab50824)
![Immunofluorescence - BBX antibody [2065C12a] (ab50824)](/ps/datasheet/images/bbxif.jpg)
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Product Name
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BBX antibody [2065C12a]
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See all BBX antibodies (2)...
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Product type
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Primary antibodies
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Description
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Mouse monoclonal [2065C12a] to BBX
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Immunogen
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Recombinant fragment (Human)
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Reacts with
(species key)
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Hu
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Tested applications
(see key)
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ICC/IF, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ICC/IF: 1/50.
WB: 1/50. Predicted molecular weight: 105 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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HeLa cells or 3T3 cells nuclear or whole celle lysate
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Cellular localization
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Nuclear
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Research areas
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Chromatin and Nuclear Signaling >> Transcription >> Transcription Factors
Chromatin and Nuclear Signaling >> Transcription >> Domain Families >> HMG Box
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Relevance
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BBX is a new mammalian protein that complements the fission yeast MBF transcription complex
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Database links
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The links below go to external sites and will open in a new browser window Entrez Gene | | 56987 (Human)
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Raised in
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Mouse
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Clonality
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Monoclonal
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Clone number
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2065C12a
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Isotype
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IgG1
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Purity
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Protein G purified
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Storage buffer
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Preservative: 0.05% Sodium Azide
Constituents: 1% BSA, PBS Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Purification notes
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ab50824 was purified from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum. Filter sterilized.
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Form
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Liquid
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Concentration
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0.100 mg/ml
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Storage instructions
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Store at 4°C for up to one year. Upon use, aliquot and store at -20°C or -80°C.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this BBX antibody [2065C12a] is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for BBX antibody [2065C12a] images, references, products related to ab50824 and other tools.
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BBX antibody [2065C12a] images:
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 Western blot - BBX antibody [2065C12a] (ab50824)
BBX antibody [2065C12a] (ab50824) at 1/50 dilution + HeLa whole cell lysate at 50 µg
Secondary
anti-mouse IgG at 1/2500 dilution
Predicted band size : 105 kDa
Observed band size : 130 kDa (why is the actual band size different from the predicted?)
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Immunofluorescence - BBX antibody [2065C12a] (ab50824)
Immunofluorescent staining of BBX expression HeLa cells using ab50824 at a 1/50 dilution and and AlexaFluor 488 conjugated goat anti-mouse secondary
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Search PubMed (MEDLINE) for references to BBX
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BBX antibody [2065C12a] - more information
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Customer reviews (feedback) regarding BBX antibody [2065C12a] |
Customer FAQs regarding BBX antibody [2065C12a] |
Protocols for BBX antibody [2065C12a] |
Price and availability of products related to BBX antibody [2065C12a] |
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BBX antibody [2065C12a] - related products:
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BBX antibody [2065C12a] - other tools:
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Contact Abcam with a Technical Enquiry about BBX antibody [2065C12a] |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Pricing information loading
![Western blot - BBX antibody [2065C12a] (ab50824)](/index.html?datasheet=50824#bbx.jpg)
![Immunofluorescence - BBX antibody [2065C12a] (ab50824)](/index.html?datasheet=50824#bbxif.jpg)
