Anti-BBX antibody [2065C12a] (ab50824)
Key features and details
- Mouse monoclonal [2065C12a] to BBX
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-BBX antibody [2065C12a]
See all BBX primary antibodies -
Description
Mouse monoclonal [2065C12a] to BBX -
Host species
Mouse -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment (Human)
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Positive control
- HeLa cells or 3T3 cells nuclear or whole celle lysate
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at 4°C (stable for up to 12 months). Store at -20°C or -80°C. -
Storage buffer
pH: 7.4
Preservative: 0.05% Sodium azide
Constituents: 1% BSA, PBS -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
ab50824 was purified from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum. Filter sterilized. -
Clonality
Monoclonal -
Clone number
2065C12a -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab50824 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/50. Predicted molecular weight: 105 kDa.
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ICC/IF |
1/50.
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Notes |
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WB
1/50. Predicted molecular weight: 105 kDa. |
ICC/IF
1/50. |
Target
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Relevance
Function: Transcription factor that is necessary for cell cycle progression from G1 to S phase. Similarity: Contains 1 HMG box DNA-binding domain. -
Cellular localization
Nuclear -
Database links
- Entrez Gene: 56987 Human
- SwissProt: A2RRM7 Human
- SwissProt: Q8WY36 Human
- Unigene: 124366 Human
- Unigene: 736885 Human
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Alternative names
- BBX antibody
- BBX_HUMAN antibody
- Bobby sox homolog antibody
see all
Images
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Anti-BBX antibody [2065C12a] (ab50824) at 1/50 dilution + HeLa whole cell lysate at 50 µg
Secondary
anti-mouse IgG at 1/2500 dilution
Predicted band size: 105 kDa
Observed band size: 130 kDa why is the actual band size different from the predicted? -
Immunofluorescent staining of BBX expression HeLa cells using ab50824 at a 1/50 dilution and and AlexaFluor 488 conjugated goat anti-mouse secondary
Protocols
Datasheets and documents
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Datasheet download
References (0)
ab50824 has not yet been referenced specifically in any publications.