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Abcam's BCA Protein Quantification Kit provides a simple, rapid, detergent tolerant (up to 5%) procedure for determining the concentration of proteins in solution. The method utilizes a copper (Cu2+) salt which can be reduced to the cuprous state by protein(s). The generated Cu2+ ion forms an intensely colored complex with the bicinchoninic acid reagent with a very strong absorbance band centered at 562 nm. The intensity of the blue complex is proportional to the amount of protein in the sample. The BCA Protein Assay is suitable for measuring protein concentration in the range of 0.5-30 µg protein (0.01-0.6 mg/ml).
|BCA Reagent||NM||1 x 100ml|
|BSA Standard||Yellow||1 x 1ml|
|Copper Reagent||Blue||1 x 2ml|
Our Abpromise guarantee covers the use of ab102536 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent dilution.|