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BCA Protein Quantification Kit (ab102536)

Q&A (13)

Overview

  • Product nameBCA Protein Quantification Kit
  • Tests
    1000 x 1 assay
  • Sample type
    Serum, Plasma, Cell culture extracts, Tissue Extracts
  • Assay typeQuantitative
  • Sensitivity
    > 0.5 µg
  • Range
    0.5 µg - 30 µg
  • Assay time
    1h 30m
  • Product overview

    Abcam's BCA Protein Quantification Kit provides a simple, rapid, detergent tolerant (up to 5%) procedure for determining the concentration of proteins in solution. The method utilizes a copper (Cu2+) salt which can be reduced to the cuprous state by protein(s). The generated Cu2+ ion forms an intensely colored complex with the bicinchoninic acid reagent with a very strong absorbance band centered at 562 nm. The intensity of the blue complex is proportional to the amount of protein in the sample. The BCA Protein Assay is suitable for measuring protein concentration in the range of 0.5-30 µg protein (0.01-0.6 mg/ml).
    Visit our FAQs page for tips and troubleshooting.

  • Tested applicationsFunctional Studies more details

Properties

  • RelevanceThe BCA Protein assay (or Smith assay) is a biochemical assay for determining the total level of protein in a solution. The total protein concentration is determined by measuring the color change of the sample solution from green to purple which is proportional to the protein concentration.
    • Alternative names
        Bicinchoninic acid protein

    Applications

    Our Abpromise guarantee covers the use of ab102536 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    Functional Studies FuncS

    BCA Protein Quantification Kit images

    • Functional Studies - BCA Protein Quantification Kit (ab102536)
      Functional Studies - BCA Protein Quantification Kit (ab102536)
      Standard Curve at 37°C.
    • Functional Studies - BCA Protein Quantification Kit (ab102536)
      Functional Studies - BCA Protein Quantification Kit (ab102536)
      Standard Curve at 60°C.

    Protocols

    References for BCA Protein Quantification Kit (ab102536)

    ab102536 has not yet been referenced specifically in any publications.

    Publishing research using ab102536 ? Please let us know so that we can cite the reference in this datasheet

    Product Wall

    Submit an Abreview for ab102536 Submit a Question for ab102536

    Displaying 1 - 10 of 13 results for Abreviews and Q&A

    10
    Votes: Highest First

    I was just wondering if you any one knows whether the BCA quantitation kit (ab102536) is compatible with reducing agents such as DTT or BME?

    If not would it be possible to confirm this with the supplier?

    Best Regards,

    Thank you for contacting us.

    If you are interested in knowing the compatibility of diffrent substances then following link will be helpful. They use the same compound for estimation as we use;

    http://genome.yale.edu/images/a/aa/BCA.pdf Read More

    Pourriez-vous nous indiquer la durée de vie de votre produit :

    REF : ab10253, BCA Protein quantification kit.

    Pourriez-vous également nous fournir le CoA de ce produit pour le lot GR48804-1 ?

    En vous remercia...

    Read More

    Merci de nous avoir contactés.
    Nos produits ne sont pas tous fabriqués en interne. Nous vendons des produits que nous pensons être d'intérêt pour nos clients qui proviennent d’autres fournisseurs ou collaborateurs universitaires. Tous ces produits so...

    Read More

    abcam gibt auf der Internetseite die Rezeptur für einen Tris-Triton Lysepuffer (Beginners Guide für Western Blots). Ich habe diesen einmal ausprobiert, finde aber keine Proteinbestimmungsmethode, die alle Inhaltsstoffe (EDTA und Triton-X) in den...

    Read More

    Vielen Dank für Ihre Anfrage.

    Ich kann bestätigen, dass die meisten Methoden zur Proteinbestimmung nicht funktionieren wenn Detergenzien enthalten sind.

    Die beste und robusteste Methode ist deshalb der BCA Protein Assay. Dies...

    Read More

    Dear xxxx,

    Thanks for all the relevant information and sparing some time for the technical suggestions.

    Best Regards,

    That is no problem at all. I hope it was of some help to you. If you could let me know the target you are looking at I can have a look at the way we prepare lysates, if we have an antibody directed towards it?

    Otherwise, I wish you all the best w...

    Read More

    Hi,

    Thanks for your suggestions. Actually, I am doing western for a transcription factor, which in literature is suggested to be having a very short half-life. So, an expert in the field suggested me to use Sample buffer directly to lyse the cell...

    Read More

    Thank you for your email and sorry for my late reply.

    I think if an expert in the field has suggested this method has worked for his group it may well be worth trying. What you could always do is try both methods of lysis and probe the samples to...

    Read More

    Dear xxxx,

    I think I will stick to the order already placed with you, as the quality what I expected from another supplier is not up to the mark. Please try your level best to deliver the same at the earliest, that is what I can say at most.
    ...

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    Thank you for your reply.

    I will then let the order through as it stands. We will endeavor to have the product with you as soon as possible but as I am sure you can understand we are reliant on delivery companies to move the package on our behalf...

    Read More

    Hi Karin,

    May be we can place order for more DTT later. But for the time being-

    1. Please place order for one unit each of the following-

    - ab13243 (rabbit polyclonal HO-1)

    - ab102536 (BCA kit)

    2. Ple...

    Read More

    Thank you for your reply.

    I have now placed your order for you. This is on the order number 1xxxxand xxxx. The DTT reducer is currently not directly in stock and it will therefore take a few weeks to reach you. I have therefore split the orders....

    Read More

    Hi Karin,

    Thanks. For the BCA kit, we are planning to use the Nanodrop machine. Is there any minituarised protocol for the available BCA kit to be used with Nanodrop or in microplate? If, so please let me know.

    I will place order for...

    Read More

    Thank you for your reply.

    The protocol provided with the BCA kit (ab102536) describes how to use the kit with a microplate reader. I have attached this document for your information. It is possible the kit could be adapted to be used with a nano...

    Read More

    Could you please provide us the COA (certificate of analysis) with expiry date for product code: AB102536 (BCA PROTEIN QUANTIFICATION KIT), batch No: GR82620 as early as possible.

    Thanks in advance.

    Best regards,

    Thank you for your telephone call and enquiry this morning.

    As discussed on the telephone, some of our antibodies are produced in-house and others are sourced from suppliers or academic collaborators whose products we believe will be of interes...

    Read More

    Thank you very much for your below reply,

    I still have a question, the working range of the below Kit (BCA Protein Quantification Kit (ab102536)) is 0.01 to 0.6mg/ml,

    Can this working range be extended as the customer wants to work i...

    Read More

    Thank you for contacting us.

    The customer can measure samples which are higher in concentration than the standard range but in order to get accurate measurements they will need to dilute the samples down to measure within the standard range (0.0...

    Read More
    

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"