BCA1 (BLC) Human ELISA Kit (ab99981)
- Product nameBCA1 (BLC) Human ELISA KitSee all BCA1 kits ...
- Tests1 x 96 well plate
- Sample typeCell culture supernatant, Serum, Plasma
- Assay typeSandwich
- Sensitivity< 1.5 pg/ml
- Range1.37 pg/ml - 1000 pg/ml
Sample specific recovery Sample type Average % Range Cell culture supernatant 95.62 84% - 105% Serum 95.79 82% - 105% Plasma 93.68 83% - 104%
- Species reactivityReacts with: Human
- Product overview
ab99981 Human BCA1 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human BCA1 in serum, plasma and cell culture supernatants. This assay employs an antibody specific for Human BCA1 coated on a 96-well plate. Standards and samples are pipetted into the wells and BCA1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human BCA1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted into the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of BCA1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Optimization may be required with urine samples.
- Tested applicationsELISA more details
- Storage instructionsStore at -20°C. Please refer to protocols.
Components Identifier 1 x 96 tests 200X HRP-Streptavidin Concentrate Item G 1 x 200µl 20X Wash Buffer Concentrate Item B 1 x 25ml Assay Diluent A diluent buffer, For Standard/Sample (serum/plasma) diluent. 1 x 30ml Assay Diluent B 5x concentrated buffer. For Standard/Sample (cell culture medium/urine) diluent. 1 x 15ml BLC Microplate (12 strips x 8 wells) coated with anti-human BLC. 1 unit Detection Antibody BLC: biotinylated anti-human BLC 2 vials HRP-Streptavidin Concentrate 200x concentrated HRP-conjugated streptavidin 1 x 200µl Human BLC Standard (recombinant) 2 vials Stop Solution Item I 1 x 8ml TMB One-Step Substrate Reagent Item H 1 x 12ml
- Research Areas
- ANGIE ANGIE2 B cell attracting chemokine 1
- B cell chemoattractant B cell homing chemokine (ligand for Burkitt's lymphoma receptor 1)B lymphocyte chemoattractant BCA 1BLCBLR1LC X C motif chemokine 13 Chemokine (C X C motif) ligand 13 Chemokine (C X C motif) ligand 13 (B cell chemoattractant) Chemokine CXC motif ligand 13 CXC chemokine BLCCXCL13 SCYB13Small inducible cytokine B subfamily (Cys X Cys motif) member 13 (B cell chemoattractant)Small inducible cytokine B13Small inducible cytokine subfamily B, member 13
- SwissProt: O43927 Human
Our Abpromise guarantee covers the use of ab99981 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
References for BCA1 (BLC) Human ELISA Kit (ab99981)
ab99981 has not yet been referenced specifically in any publications.