BCA1 (BLC) Mouse ELISA Kit (ab100671)
- Product nameBCA1 (BLC) Mouse ELISA KitSee all BCA1 kits ...
- Tests1 x 96 well plate
- Sample typeCell culture supernatant, Serum, Plasma
- Assay typeSandwich
- Sensitivity< 2 pg/ml
- Range1.37 pg/ml - 1000 pg/ml
Sample specific recovery Sample type Average % Range Cell culture supernatant 104.56 90% - 110% Serum 96.89 83% - 103% Plasma 97.65 84% - 104%
- Species reactivityReacts with: Mouse
- Product overview
ab100671 mouse BCA1 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse BCA1 in serum, plasma, cell culture supernatants and urine. This assay employs an antibody specific for mouse BCA1 coated on a 96-well plate. Standards and samples are pipette into the wells and BCA1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse BCA1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of BCA1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
- Tested applicationsELISA more details
- Storage instructionsStore at -20°C. Please refer to protocols.
Components Identifier 1 x 96 tests 200X HRP-Streptavidin Concentrate Item G 1 x 200µl 20X Wash Buffer Concentrate Item B 1 x 25ml Assay Diluent A animal serum . For Standard/Sample (serum/plasma) diluent. 1 x 30ml Assay Diluent B 5x concentrated buffer. For Standard/Sample (cell culture medium/urine) diluent. 1 x 15ml BCA1 Microplate (12 strips x 8 wells) coated with anti-mouse BCA1 1 unit Detection Antibody BCA1: biotinylated anti-mouse BCA1 2 vials Mouse BCA1 Standard (recombinant) 2 vials Stop Solution Item I 1 x 8ml TMB One-Step Substrate Reagent Item H 1 x 12ml
- Research Areas
- ANGIE ANGIE2 B cell attracting chemokine 1
- B cell chemoattractant B cell homing chemokine (ligand for Burkitt's lymphoma receptor 1)B lymphocyte chemoattractant BCA 1BLCBLR1LC X C motif chemokine 13 Chemokine (C X C motif) ligand 13 Chemokine (C X C motif) ligand 13 (B cell chemoattractant) Chemokine CXC motif ligand 13 CXC chemokine BLCCXCL13 SCYB13Small inducible cytokine B subfamily (Cys X Cys motif) member 13 (B cell chemoattractant)Small inducible cytokine B13Small inducible cytokine subfamily B, member 13
Our Abpromise guarantee covers the use of ab100671 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
References for BCA1 (BLC) Mouse ELISA Kit (ab100671)
ab100671 has not yet been referenced specifically in any publications.