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Western blot - BCAR1 antibody [M144] (ab31831)
![Western blot - BCAR1 antibody [M144] (ab31831)](/ps/datasheet/images/ab31831_1.jpg)
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Product Name
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BCAR1 antibody [M144]
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See all BCAR1 antibodies (15)...
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Product type
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Primary antibodies
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Description
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Mouse monoclonal [M144] to BCAR1
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Immunogen
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Recombinant protein containing amino acid residues in the C-terminal region of rat p130 Cas.
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Reacts with
(species key)
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Hu, Ms, Rat, Chk
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Specificity
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ab31831 detects a 130 kDa protein corresponding to the molecular mass of p130 Cas on SDS-PAGE immunoblots of human A431, endothelial, and Hct116 cells.
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Epitope
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ab31831 recognises an epitope located in teh C terminal region of BCAR1.
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Tested applications
(see key)
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ELISA, IHC-P, IP, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ELISA: 1/2000.
IHC-P: 1/250.
IP: Use at a concentration of 5 - 10 µg/ml.
WB: 1/1000. Predicted molecular weight: 93 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Human A431, endothelial, and Hct116 cells.
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Cellular localization
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Focal adhesions. Unphosphorylated form localizes in the cytoplasm and can move to the membrane upon tyrosine phosphorylation.
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Research areas
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Cancer >> Signal transduction >> Other
Signal Transduction >> Protein Phosphorylation >> Tyrosine Kinases >> Other
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Relevance
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BCAR1 has a unique structure that contains a Src homology (SH)3 domain followed by multiple YXXP motifs and a proline rich region. It is a docking protein which plays a central coordinating role for tyrosine kinase based signaling related to cell adhesion. It is implicated in a variety of biological processes including cell adhesion, cell migration, growth factor stimulation, cytokine receptor engagement, bacterial infection, cardiovascular development, actin filament assembly and Src induced cell transformation. Overexpression confers antiestrogen resistance on breast cancer cells.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Mouse
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Clonality
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Monoclonal
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Clone number
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M144
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Isotype
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IgG1
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Purity
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Protein A purified
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Storage buffer
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Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol, PBS, 1mg/ml BSA Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS
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Form
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Liquid
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this BCAR1 antibody [M144] is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for BCAR1 antibody [M144] images, references, products related to ab31831 and other tools.
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BCAR1 antibody [M144] images:
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 Western blot - BCAR1 antibody [M144] (ab31831)
Predicted band size : 93 kDa
Western blot analysis of human endothelial cells serum starved overnight (lanes 1, 3, & 5) or treated with pervanadate (1 mM) for 30 minutes (lanes 2, 4, & 6). The blot was probed with anti-BCAR1 (ab31831; lanes 1 & 2), anti-BCAR1 (Tyr-751) (lanes 3 & 4) or anti-BCAR1 (Tyr-762) (lanes 5 & 6).
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Search PubMed (MEDLINE) for references to BCAR1
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BCAR1 antibody [M144] - more information
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Customer reviews (feedback) regarding BCAR1 antibody [M144] |
Customer FAQs regarding BCAR1 antibody [M144] |
Protocols for BCAR1 antibody [M144] |
Price and availability of products related to BCAR1 antibody [M144] |
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BCAR1 antibody [M144] - related products:
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BCAR1 antibody [M144] - other tools:
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Contact Abcam with a Technical Enquiry about BCAR1 antibody [M144] |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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![Western blot - BCAR1 antibody [M144] (ab31831)](/index.html?datasheet=31831#ab31831_1.jpg)
