BCAR1 antibody (ab49635)

Product Name 

BCAR1 antibody

Product type 

Primary antibodies

Description 

Rabbit polyclonal to BCAR1

Immunogen 

Synthetic peptide KFTSQDSPDGQYENSEGG corresponding to the C-terminal region of human p130Cas (amino acids 642-659) conjugated to KLH.
BLAST 'KFTSQDSPDGQYENSEGG' with or

Reacts with 
(species key)

Hu, Ms, Rat

Specificity 

Sequence has no homology to Cas-related proteins Hef1/Cas-L and Efs/Sin.

Tested applications 
(see key)

IP, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
IP: Use at an assay dependent dilution.
WB: 1/1000. Predicted molecular weight: 130 kDa.


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

Whole extract of the human ECV304 endothelial cells, a whole extract of the mouse fibroblasts NIH3T3 cell line and a whole extract of the rat skeletal muscle myoblasts L8 cell line and ECV304 human endothelial cell lysate.

Cellular localization 

Focal adhesions. Unphosphorylated form localizes in the cytoplasm and can move to the membrane upon tyrosine phosphorylation.

Research areas 

Cancer >> Signal transduction >> Other
Signal Transduction >> Protein Phosphorylation >> Tyrosine Kinases >> Other

Relevance 

BCAR1 has a unique structure that contains a Src homology (SH)3 domain followed by multiple YXXP motifs and a proline rich region. It is a docking protein which plays a central coordinating role for tyrosine kinase based signaling related to cell adhesion. It is implicated in a variety of biological processes including cell adhesion, cell migration, growth factor stimulation, cytokine receptor engagement, bacterial infection, cardiovascular development, actin filament assembly and Src induced cell transformation. Overexpression confers antiestrogen resistance on breast cancer cells.

Database links 

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Ion Exchange Chromatography

Storage buffer 

Preservative: 15mM Sodium Azide
Constituents: 0.01M PBS, pH 7.4

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Purification notes 

Whole antiserum is fractionated and then further purified by ion-exchange chromatography to provide the IgG fraction of antiserum that is essentially free of other rabbit serum proteins.

Form 

Liquid

Concentration 

5.900 mg/ml

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

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