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Reassurance, Refunds & Replacements
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »This product is covered by the Abpromise guarantee. Our scientific support team are available to answer any questions or queries - fill out an inquiry form for ab80016 for help.
There are no answered questions for ab80016
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Western blot - BCAR1 antibody (ab80016)
All lanes : Anti-BCAR1 antibody (ab80016) at 1 µg/ml
Lane 1 :
Lane 2 :
Lane 3 :
Lane 4 : MDA-MB-231 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lane 5 : MDA-MB-361 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lane 6 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lane 7 :
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 93 kDa
Observed band size : 100 kDa (why is the actual band size different from the predicted?)
Additional bands at : 37 kDa,50 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 30 seconds
Breast cancer anti-estrogen resistance protein 1
(BCAR1) contains a number of potential phosphorylation sites (SwissProt) which may explain its migration at a higher (100 kDa) molecular weight than predicted (93 kDa).
Immunocytochemistry/ Immunofluorescence - BCAR1 antibody (ab80016)
ICC/IF image of ab80016 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab80016, 1µg/ml) overnight at +4ºC. The secondary antibody (green) was Alexa Fluor® 488 Goat anti-Rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) HEK293, HepG2, and MCF-7 cells at 1µg/ml, and in 100% Methanol fixed (5 min) HeLa, HEK293, HepG2, and MCF-7 cells at 1µg/ml.
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