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Products:Signal Transduction >> Protein Phosphorylation >> Tyrosine Kinases >> Other
Overview
Product name
Anti-BCAR1 antibody
See all BCAR1 products (17) ...
Description
Rabbit polyclonal to BCAR1
Tested applications
WB, ICC/IFmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Rat, Cow, Zebrafish
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human BCAR1.
(Peptide available as ab89387.)
Positive control
This antibody gave a positive signal in the following tissue lysates: Human Brain; Human Testis; Human Liver. Whole cell lysates: MBA MD 231; MDA MB 361; MCF7; T47D.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Signal transduction >> Other
Signal Transduction >> Protein Phosphorylation >> Tyrosine Kinases >> Other
Applications
Show applications key
Our Abpromise guarantee covers the use of ab80016 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 1 µg/mlDetects a band of approximately 100 kDa (predicted molecular weight: 93 kDa).
ICC/IF: Use a concentration of 1 µg/ml
Target
Function
Docking protein which plays a central coordinating role for tyrosine-kinase-based signaling related to cell adhesion. Implicated in induction of cell migration. Overexpression confers antiestrogen resistance on breast cancer cells.
Tissue specificity
Widely expressed with an abundant expression in the testis. Low level of expression seen in the liver, thymus, and peripheral blood leukocytes. The protein has been detected in a B-cell line.
Sequence similarities
Belongs to the CAS family.
Contains 1 SH3 domain.
Domain
Contains a central domain (substrate domain) containing multiple potential SH2-binding sites and a C-terminal domain containing a divergent helix-loop-helix (HLH) motif. The SH2-binding sites putatively bind CRK, NCK and ABL SH2 domains. The HLH motif is absolutely required for the induction of pseudohyphal growth in yeast and mediates heterodimerization with CASL.
A serine-rich region promotes activation of the serum response element (SRE).
The SH3 domain is necessary for the localization of the protein to focal adhesions and interacts with one proline-rich region of focal adhesion kinase 1.
Post-translational
modifications
Focal adhesion kinase 1 phosphorylates the protein at the YDYVHL motif. SRC-family kinases are recruited to the phosphorylated sites and can phosphorylate other tyrosine residues. Tyrosine phosphorylation is triggered by integrin mediated adhesion of cells to the extracellular matrix.
Cellular localization
Cell junction > focal adhesion. Cytoplasm. Unphosphorylated form localizes in the cytoplasm and can move to the membrane upon tyrosine phosphorylation.
Target information above from: UniProt accessionP56945
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Breast cancer anti estrogen resistance 1 protein antibody
- BCAR 1 antibody
- Bcar1 antibody
see all
Database links
- Entrez Gene: 9564 Human
- Entrez Gene: 12927 Mouse
- Entrez Gene: 25414 Rat
- Omim: 602941 Human
- SwissProt: P56945 Human
- SwissProt: Q61140 Mouse
- SwissProt: Q63767 Rat
- Unigene: 479747 Human
see all
Anti-BCAR1 antibody images:
Western blot - BCAR1 antibody (ab80016)
All lanes : Anti-BCAR1 antibody (ab80016) at 1 µg/ml
Lane 1 : Breast (Human) Tissue Lysate - normal tissue (ab30090)
Lane 2 : Testis (Human) Tissue Lysate - adult normal tissue (ab30257)
Lane 3 : Liver (Human) Tissue Lysate - adult normal tissue (ab29889)
Lane 4 : MDA-MB-231 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lane 5 : MDA-MB-361 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lane 6 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lane 7 : T47D (Human ductal breast epithelial tumor cell line) Whole Cell Lysate (ab14899)
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 93 kDa
Observed band size : 100 kDa (why is the actual band size different from the predicted?)
Additional bands at : 37 kDa,50 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 30 seconds
Breast cancer anti-estrogen resistance protein 1
(BCAR1) contains a number of potential phosphorylation sites (SwissProt) which may explain its migration at a higher (100 kDa) molecular weight than predicted (93 kDa).
Immunocytochemistry/ Immunofluorescence - BCAR1 antibody (ab80016)
ICC/IF image of ab80016 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab80016, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 Goat anti-Rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) HEK293, HepG2, and MCF-7 cells at 1µg/ml, and in 100% Methanol fixed (5 min) HeLa, HEK293, HepG2, and MCF-7 cells at 1µg/ml.
References for Anti-BCAR1 antibody (ab80016)
ab80016 has not yet been referenced specifically in any publications.
Publishing research using ab80016? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"