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Western blot - BCAR3 antibody (ab56347)
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Product Name
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BCAR3 antibody
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See all BCAR3 antibodies (4)...
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Product type
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Primary antibodies
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Description
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Mouse monoclonal to BCAR3
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Immunogen
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Recombinant fragment, corresponding to amino acids 266-373 of Human BCAR3
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Reacts with
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Hu
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Tested applications
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WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
WB: Use at a concentration of 1-5 µg/ml.
This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Research areas
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Tags & Cell Markers >> Cell Type Markers >> Tumor Associated
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Relevance
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BCAR3 is a component of intracellular signal transduction that causes estrogen-independent proliferation in human breast cancer cells. The protein contains a putative src homology 2 (SH2) domain, a hall mark of cellular tyrosine kinase signaling molecules, and is partly homologous to the cell division cycle protein CDC48. When overexpressed, it confers anti-estrogen resistance in breast cancer cell lines.
In breast cancer, the antiestrogen tamoxifen has been prescribed for both primary treatment and the treatment of advanced metastatic disease. Although the drug induces remission in most patients with estrogen receptor-positive disease, all patients eventually develop resistance. van Agthoven et al. (1998) applied an insertional mutagenesis strategy to a breast cancer cell line. Infected cells were subjected to tamoxifen selection, and the resistant clones were screened for a common integration site linked with antiestrogen resistance. By screening a human testis cDNA library with the integration site-specific probe, they obtained a cDNA encoding BCAR3 (breast cancer antiestrogen resistance 3). The deduced 825 amino acid BCAR3 protein contains a putative Src homology 2 (SH2) domain and sequences homologous to yeast CDC48. Northern blot analysis detected abundant expression of a 3.4kb BCAR3 transcript in heart, placenta, skeletal muscle, spleen, prostate, testis, ovary, small intestine, colon, fetal kidney, and several cancer cell lines, but not in nonmalignant breast tissue; a 6kb BCAR3 transcript was also detected in skeletal muscle and heart.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Mouse
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Clonality
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Monoclonal
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Isotype
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IgG2a
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Light chain type
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kappa
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Purity
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Protein G purified
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Storage buffer
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Preservative: None
PBS, pH 7.2
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this BCAR3 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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BCAR3 antibody images:
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 Western blot - BCAR3 antibody (ab56347)
Western blot against tagged recombinant protein immunogen using ab56347 BCAR3 antibody at 1ug/ml. Predicted band size of immunogen is 38 kDa
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Search PubMed (MEDLINE) for references to BCAR3
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BCAR3 antibody - more information
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Customer reviews (feedback) regarding BCAR3 antibody |
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Protocols for BCAR3 antibody |
Price and availability of products related to BCAR3 antibody |
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BCAR3 antibody - other tools:
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Contact Abcam with a Technical Enquiry about BCAR3 antibody |
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