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If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »Publishing research using ab65111? Please let us know so that we can cite the reference in this datasheet
ab65111 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - BCAR3 antibody (ab65111)
Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue stained with BCAR3 antibody (ab65111) at 1/50 - 1/100 dilution, with (right) and without (left) the immunising peptide.
Western blot - BCAR3 antibody (ab65111)
All lanes : Anti-BCAR3 antibody (ab65111) at 1/500 dilution
Lane 1 : extracts from HeLa cells
Lane 2 : extracts from Jurkat cells
Lane 3 : extracts from HUVEC cells
Lane 4 : extracts from HUVEC cells with immunising peptide at 5 µg
Lysates/proteins at 5 µg per lane.
Predicted band size : 93 kDa
Observed band size : 93 kDa
Immunocytochemistry/ Immunofluorescence-BCAR3 antibody(ab65111)
ICC/IF image of ab65111 stained MCF7 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab65111, 1µg/ml) overnight at +4ºC. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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