![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - BCL2L1 antibody [2H12] - BSA and Azide free (ab77571) image](#bclx.bmp)
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Product name
Anti-BCL2L1 antibody [2H12] - BSA and Azide free
See all BCL2L1 products (29) ...
Description
Mouse monoclonal [2H12] to BCL2L1 - BSA and Azide free
Tested applications
WB, IHC-P, Flow Cyt, ICC/IFmore details
Cross reactivity
Reacts with
Mouse, Rat, Human, Pig
Predicted to work with
Horse, Cow, Cat, Macaque Monkey
Immunogen
Synthetic peptide: Cys-QSNRELVVDFLS, corresponding to amino acids 3-14 of Human BCL2L1
Cys-QSNRELVVDFLS
Epitope
aa 3-14
Positive control
Hodgkin’s lymphoma
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 10mM PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Protein A purified
Purification notes
Purified from the ascites fluid
Clonality
Monoclonal
Clone number
2H12
Isotype
IgG2a
Research areas
Metabolism >> Types of disease >> Cancer
Metabolism >> Pathways and Processes >> Metabolism processes >> Apoptosis
Metabolism >> Pathways and Processes >> Metabolism processes >> Hypoxia
Metabolism >> Pathways and Processes >> Mitochondrial Metabolism >> Mitochondrial markers
Cancer >> Cancer Metabolism >> Response to hypoxia
Cancer >> Tumor biomarkers >> Oncoproteins
Cancer >> Oncoproteins/suppressors >> Oncoproteins >> Cell survival & death
Cancer >> Invasion/microenvironment >> Apoptosis >> Bcl 2 family
Signal Transduction >> Metabolism >> Mitochondrial
Cell Biology >> Apoptosis >> Intracellular >> Bcl2 Family
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - BCL2L1 antibody [2H12] - BSA and Azide free (ab77571)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - BCL2L1 antibody [2H12] - BSA and Azide free (ab77571) image](/ps/datasheet/Images/77/ab77571/bclx.bmp)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab77571 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB
- 1 Image
IHC-P
Flow Cyt
ICC/IF
Application notes
Flow Cyt: Use at an assay dependent dilution.
ICC/IF: Use at an assay dependent dilution.
IHC-P: Use at a concentration of 2 - 4 µg/ml for 30 min at RT. Perform heat mediated antigen retrieval before commencing with IHC staining protocol, by boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.
WB: (Not optimal) Use at an assay dependent dilution. Predicted molecular weight: 26 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Potent inhibitor of cell death. Inhibits activation of caspases (By similarity). Appears to regulate cell death by blocking the voltage-dependent anion channnel (VDAC) by binding to it and preventing the release of the caspase activator, CYC1, from the mitochondrial membrane.
Isoform Bcl-X(S) promotes apoptosis.
Tissue specificity
Bcl-X(S) is expressed at high levels in cells that undergo a high rate of turnover, such as developing lymphocytes. In contrast, Bcl-X(L) is found in tissues containing long-lived postmitotic cells, such as adult brain.
Sequence similarities
Belongs to the Bcl-2 family.
Domain
The BH4 motif is required for anti-apoptotic activity. The BH1 and BH2 motifs are required for both heterodimerization with other Bcl-2 family members and for repression of cell death.
Post-translational
modifications
Proteolytically cleaved by caspases during apoptosis. The cleaved protein, lacking the BH4 motif, has pro-apoptotic activity.
Cellular localization
Mitochondrion membrane. Nucleus membrane. Mitochondrial membranes and perinuclear envelope.
Target information above from: UniProt accessionQ07817
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Apoptosis regulator Bcl X antibody
- Apoptosis regulator Bcl-X antibody
- Apoptosis regulator BclX antibody
see all
Database links
- Entrez Gene: 282152 Cow
- Entrez Gene: 598 Human
- Entrez Gene: 12048 Mouse
- Entrez Gene: 397536 Pig
- Entrez Gene: 24888 Rat
- GenBank: CAA80661 Human
- Omim: 600039 Human
- SwissProt: Q5TE63 Human
see all
Anti-BCL2L1 antibody [2H12] - BSA and Azide free images:
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - BCL2L1 antibody [2H12] - BSA and Azide free (ab77571)
Formalin-fixed, paraffin-embedded human Hodgkin’s lymphoma stained with 2-4ug/ml ab77571, using peroxidase-conjugate and AEC chromogen. Note cell membrane staining of Reed-Sternberg cells.
References for Anti-BCL2L1 antibody [2H12] - BSA and Azide free (ab77571)
This product has been referenced in:
- Wolter KG et al. Movement of Bax from the cytosol to mitochondria during apoptosis. J Cell Biol 139:1281-92 (1997). IF.Read more (PubMed: 9382873) »
See 1 publication for this product
Publishing research using ab77571? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"