BCL2L1 antibody [2H12] (ab9478)

Product Name 

BCL2L1 antibody [2H12]

Product type 

Primary antibodies

Description 

Mouse monoclonal [2H12] to BCL2L1

Immunogen 

Synthetic peptide: C-QSNRELVVDFLS, corresponding to amino acids 3-14 of Human Bcl x.
BLAST 'C-QSNRELVVDFLS' with or

Reacts with 
(species key)

Hu, Ms, Rat, Pig

Specificity 

This antibody recognizes a 27 kDa protein identified as bcl-X protein.
This antibody is highly specific to bcl-X and does not show cross reactivity with bcl-2 alpha or bax protein.

Tested applications 
(see key)

IHC-Fr, IHC-P, IP, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
Immunohistochemistry: 1 / 20 - 1 / 50 for 30 - 60 mins at RT (ABC method).
Immunoprecipitation: 1 / 50.
Western Blot: 1 / 50 - 1 / 100.
Not tested in other applications.

Optimal dilutions / concentrations should be determined by the end user.

By Western blot, this antibody detects a band of 27 kDa, which corresponds to the predicted molecular weight of Bcl-xL, and a band of 19 kDa, which corresponds to the predicted molecular weight of Bcl-xS.

Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10mM citrate buffer pH 6.0 prior to immunostaining.

Positive control 
(see definition)

Hodgkin’s lymphoma.

Cellular localization 

Mitochondrial membranes and perinuclear envelope

Research areas 

Cancer >> Cancer Metabolism >> Response to hypoxia
Cancer >> Tumor biomarkers >> Oncoproteins
Cancer >> Oncoproteins/suppressors >> Oncoproteins >> Cell survival & death
Cancer >> Invasion/microenvironment >> Apoptosis >> Bcl 2 family
Signal Transduction >> Metabolism >> Mitochondrial
Cell Biology >> Apoptosis >> Intracellular >> Bcl2 Family

Relevance 

The protein encoded by this gene belongs to the Bcl2 protein family. Bcl2 family members form hetero or homodimers and act as anti or pro apoptotic regulators that are involved in a wide variety of cellular activities. The proteins encoded by this gene are located at the outer mitochondrial membrane, and have been shown to regulate outer mitochondrial membrane channel (VDAC) opening. VDAC regulates mitochondrial membrane potential, and thus controls the production of reactive oxygen species and release of cytochrome C by mitochondria, both of which are the potent inducers of cell apoptosis. Two alternatively spliced transcript variants, which encode distinct isoforms, have been reported. The longer isoform acts as an apoptotic inhibitor and the shorter form acts as an apoptotic activator.

Database links 

Raised in 

Mouse

Clonality 

Monoclonal

Clone number 

2H12

Isotype 

IgG2a

Purity 

Tissue culture supernatant

Storage buffer 

n/a

Form 

Liquid

Concentration 

Concentration not determined - why is this?

Storage instructions 

Store at +4°C.

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