![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - BCL2L1 antibody [2H12] (ab9478) image](#ab9478_1.jpg)
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Products:Cell Biology >> Apoptosis >> Intracellular >> Bcl2 Family
Overview
Product name
Anti-BCL2L1 antibody [2H12]
See all BCL2L1 products (30) ...
Description
Mouse monoclonal [2H12] to BCL2L1
Specificity
This antibody recognizes a 27 kDa protein identified as bcl-X protein. This antibody is highly specific to bcl-X and does not show cross reactivity with bcl-2 alpha or bax protein.
Tested applications
IHC-P, IHC-Fr, IP, WBmore details
Cross reactivity
Reacts with
Mouse, Rat, Human, Pig
Immunogen
Synthetic peptide: C-QSNRELVVDFLS, corresponding to amino acids 3-14 of Human Bcl x.
C-QSNRELVV DFLS
Positive control
Hodgkin’s lymphoma.
Properties
Form
Liquid
Storage instructions
Store at +4°C.
Purity
Tissue culture supernatant
Clonality
Monoclonal
Clone number
2H12
Myeloma
unknown
Isotype
IgG2a
Light chain type
unknown
Research areas
Cancer >> Cancer Metabolism >> Response to hypoxia
Cancer >> Tumor biomarkers >> Oncoproteins
Cancer >> Oncoproteins/suppressors >> Oncoproteins >> Cell survival & death
Cancer >> Invasion/microenvironment >> Apoptosis >> Bcl 2 family
Signal Transduction >> Metabolism >> Mitochondrial
Cell Biology >> Apoptosis >> Intracellular >> Bcl2 Family
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - BCL2L1 antibody [2H12] (ab9478)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - BCL2L1 antibody [2H12] (ab9478) image](/ps/datasheet/Images/9/ab9478/ab9478_1.jpg)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab9478 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: 1/20 - 1/50.Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
IHC-Fr: Use at an assay dependent dilution.
IP
IP: 1/50IP: 1/50
WB
WB: 1/50 - 1/100.WB: 1/50 - 1/100.
Target
Function
Potent inhibitor of cell death. Inhibits activation of caspases (By similarity). Appears to regulate cell death by blocking the voltage-dependent anion channnel (VDAC) by binding to it and preventing the release of the caspase activator, CYC1, from the mitochondrial membrane.
Isoform Bcl-X(S) promotes apoptosis.
Tissue specificity
Bcl-X(S) is expressed at high levels in cells that undergo a high rate of turnover, such as developing lymphocytes. In contrast, Bcl-X(L) is found in tissues containing long-lived postmitotic cells, such as adult brain.
Sequence similarities
Belongs to the Bcl-2 family.
Domain
The BH4 motif is required for anti-apoptotic activity. The BH1 and BH2 motifs are required for both heterodimerization with other Bcl-2 family members and for repression of cell death.
Post-translational
modifications
Proteolytically cleaved by caspases during apoptosis. The cleaved protein, lacking the BH4 motif, has pro-apoptotic activity.
Cellular localization
Mitochondrion membrane. Nucleus membrane. Mitochondrial membranes and perinuclear envelope.
Target information above from: UniProt accessionQ07817
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Apoptosis regulator Bcl X antibody
- Apoptosis regulator Bcl-X antibody
- Apoptosis regulator BclX antibody
see all
Database links
- Entrez Gene: 598 Human
- Entrez Gene: 12048 Mouse
- Entrez Gene: 397536 Pig
- Entrez Gene: 24888 Rat
- GenBank: CAA80661 Human
- Omim: 600039 Human
- SwissProt: Q07817 Human
- SwissProt: Q5TE63 Human
see all
Anti-BCL2L1 antibody [2H12] images:
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - BCL2L1 antibody [2H12] (ab9478)
ab9478s staining human Hodgkins lymphoma by IHC-P.
References for Anti-BCL2L1 antibody [2H12] (ab9478)
ab9478 has not yet been referenced specifically in any publications.
Publishing research using ab9478? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"