![Flow Cytometry-Anti-BCL2L1 antibody [7B2.5] (FITC)(ab26148) image](#BCL2L1-Primary-antibodies-ab26148-1.jpg)
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Product name
Anti-BCL2L1 antibody [7B2.5] (FITC)
See all BCL2L1 products (29) ...
Description
Mouse monoclonal [7B2.5] to BCL2L1 (FITC)
Conjugation
FITC
Tested applications
Flow Cyt, IHC-Fr, IP, WBmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
Recombinant full length protein (Human)
Properties
Form
Liquid
Storage instructions
Store at +4°C in the dark.
Storage buffer
Preservative: 0.1% Sodium Azide
Constituents: PBS
Concentration
Concentration information loading...
Purity
IgG fraction
Clonality
Monoclonal
Clone number
7B2.5
Isotype
IgG3
Research areas
Metabolism >> Types of disease >> Cancer
Metabolism >> Pathways and Processes >> Metabolism processes >> Apoptosis
Metabolism >> Pathways and Processes >> Metabolism processes >> Hypoxia
Metabolism >> Pathways and Processes >> Mitochondrial Metabolism >> Mitochondrial markers
Cancer >> Cancer Metabolism >> Response to hypoxia
Cancer >> Tumor biomarkers >> Oncoproteins
Cancer >> Oncoproteins/suppressors >> Oncoproteins >> Cell survival & death
Cancer >> Invasion/microenvironment >> Apoptosis >> Bcl 2 family
Signal Transduction >> Metabolism >> Mitochondrial
Cell Biology >> Apoptosis >> Intracellular >> Bcl2 Family
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Flow Cytometry-Anti-BCL2L1 antibody [7B2.5] (FITC)(ab26148)
![Flow Cytometry-Anti-BCL2L1 antibody [7B2.5] (FITC)(ab26148) image](/ps/datasheet/images/26/ab26148/BCL2L1-Primary-antibodies-ab26148-1.jpg)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab26148 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Flow Cyt: Use 1µg for 106 cells.
IHC-Fr: Use at an assay dependent dilution.
IP: Use at an assay dependent dilution.
WB: Use at an assay dependent dilution. Predicted molecular weight: 29 kDa.
Target
Function
Potent inhibitor of cell death. Inhibits activation of caspases (By similarity). Appears to regulate cell death by blocking the voltage-dependent anion channnel (VDAC) by binding to it and preventing the release of the caspase activator, CYC1, from the mitochondrial membrane.
Isoform Bcl-X(S) promotes apoptosis.
Tissue specificity
Bcl-X(S) is expressed at high levels in cells that undergo a high rate of turnover, such as developing lymphocytes. In contrast, Bcl-X(L) is found in tissues containing long-lived postmitotic cells, such as adult brain.
Sequence similarities
Belongs to the Bcl-2 family.
Domain
The BH4 motif is required for anti-apoptotic activity. The BH1 and BH2 motifs are required for both heterodimerization with other Bcl-2 family members and for repression of cell death.
Post-translational
modifications
Proteolytically cleaved by caspases during apoptosis. The cleaved protein, lacking the BH4 motif, has pro-apoptotic activity.
Cellular localization
Mitochondrion membrane. Nucleus membrane. Mitochondrial membranes and perinuclear envelope.
Target information above from: UniProt accessionQ07817
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Apoptosis regulator Bcl X antibody
- Apoptosis regulator Bcl-X antibody
- Apoptosis regulator BclX antibody
see all
Database links
- Entrez Gene: 598 Human
- Entrez Gene: 12048 Mouse
- Entrez Gene: 24888 Rat
- GenBank: CAA80661 Human
- Omim: 600039 Human
- SwissProt: Q5TE63 Human
- SwissProt: Q07817 Human
- SwissProt: Q64373 Mouse
see all
Anti-BCL2L1 antibody [7B2.5] (FITC) images:
Flow Cytometry-Anti-BCL2L1 antibody [7B2.5] (FITC)(ab26148)
Overlay histogram showing DU145 cells stained with ab26148 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab26148, 1µg/1x106 cells) for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG3 (1µg/1x106 cells ). Acquisition of >5,000 events was performed.
References for Anti-BCL2L1 antibody [7B2.5] (FITC) (ab26148)
ab26148 has not yet been referenced specifically in any publications.
Publishing research using ab26148? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"