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Products:Cell Biology >> Apoptosis >> Intracellular >> Bcl2 Family
Overview
Product name
Anti-BCL2L1 antibody [7B2.5] (Phycoerythrin)
See all BCL2L1 products (30) ...
Description
Mouse monoclonal [7B2.5] to BCL2L1 (Phycoerythrin)
Conjugation
Phycoerythrin
Specificity
ab26035 recognises Bcl xL.
Tested applications
Flow Cyt, WB, IPmore details
Cross reactivity
Reacts with
Mouse, Human
Immunogen
Recombinant full length protein: Bcl xL (Human)
Properties
Form
Liquid
Storage instructions
Store at +4°C in the dark. Do not freeze.
Storage buffer
Preservative: 0.09% Sodium Azide
Constituents: 16% Sucrose, PBS and stabilising agent.
Concentration
Concentration information loading...
Purity
IgG fraction
Clonality
Monoclonal
Clone number
7B2.5
Isotype
IgG3
Research areas
Cancer >> Cancer Metabolism >> Response to hypoxia
Cancer >> Tumor biomarkers >> Oncoproteins
Cancer >> Oncoproteins/suppressors >> Oncoproteins >> Cell survival & death
Cancer >> Invasion/microenvironment >> Apoptosis >> Bcl 2 family
Signal Transduction >> Metabolism >> Mitochondrial
Cell Biology >> Apoptosis >> Intracellular >> Bcl2 Family
Applications
Show applications key
Our Abpromise guarantee covers the use of ab26035 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Flow Cyt: Use 0.3µg for 106 cells.
WB: Use at an assay dependent dilution. Predicted molecular weight: 26 kDa.
IP: Use at an assay dependent dilution.
Target
Function
Potent inhibitor of cell death. Inhibits activation of caspases (By similarity). Appears to regulate cell death by blocking the voltage-dependent anion channnel (VDAC) by binding to it and preventing the release of the caspase activator, CYC1, from the mitochondrial membrane.
Isoform Bcl-X(S) promotes apoptosis.
Tissue specificity
Bcl-X(S) is expressed at high levels in cells that undergo a high rate of turnover, such as developing lymphocytes. In contrast, Bcl-X(L) is found in tissues containing long-lived postmitotic cells, such as adult brain.
Sequence similarities
Belongs to the Bcl-2 family.
Domain
The BH4 motif is required for anti-apoptotic activity. The BH1 and BH2 motifs are required for both heterodimerization with other Bcl-2 family members and for repression of cell death.
Post-translational
modifications
Proteolytically cleaved by caspases during apoptosis. The cleaved protein, lacking the BH4 motif, has pro-apoptotic activity.
Cellular localization
Mitochondrion membrane. Nucleus membrane. Mitochondrial membranes and perinuclear envelope.
Target information above from: UniProt accessionQ07817
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Apoptosis regulator Bcl X antibody
- Apoptosis regulator Bcl-X antibody
- Apoptosis regulator BclX antibody
see all
Database links
- Entrez Gene: 598 Human
- Entrez Gene: 12048 Mouse
- GenBank: CAA80661 Human
- Omim: 600039 Human
- SwissProt: Q07817 Human
- SwissProt: Q5TE63 Human
- SwissProt: Q64373 Mouse
- Unigene: 516966 Human
see all
References for Anti-BCL2L1 antibody [7B2.5] (Phycoerythrin) (ab26035)
This product has been referenced in:
- Henderson RBet al. A novel Rac-dependent checkpoint in B cell development controls entry into the splenic white pulp and cell survival. J Exp Med 207:837-53 (2010). Flow Cyt; Mouse.Read more (PubMed: 20308364) »
- Peperzak Vet al. The Pim kinase pathway contributes to survival signaling in primed CD8+ T cells upon CD27 costimulation. J Immunol 185:6670-8 (2010). WB; Mouse.Read more (PubMed: 21048108) »
See all 3 publications for this product
Publishing research using ab26035? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"