BCL2L1 antibody [E18] (ab32370)

Product Name 

BCL2L1 antibody [E18]

Product type 

Primary antibodies

Description 

Rabbit monoclonal [E18] to BCL2L1

Immunogen 

A synthetic peptide corresponding to residues between BH3 and BH4 of Human Bcl-x.

Reacts with 

Hu, Ms, Rat

Predicted to react 
(species key)

Pig
(Due to sequence homology)

Specificity 

This antibody should recognize Bcl-xL, Bcl-xS and Bcl-x(beta) as the immunogen sequence is common to them. The antibody does not cross-react with other Bcl-2 family members.

Tested applications 
(see key)

Flow Cyt, ICC/IF, IHC-P, IP, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
Flow Cyt: 1/50.
ICC/IF: 1/100.
IHC-P: 1/50 - 1/100.
IP: 1/10.
WB: 1/1000. Detects a band of approximately 26 kDa.


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

Human prostate carcinoma tissue and K562 cells.

Cellular localization 

Mitochondrial membranes and perinuclear envelope

Research areas 

Cancer >> Cancer Metabolism >> Response to hypoxia
Cancer >> Tumor biomarkers >> Oncoproteins
Cancer >> Oncoproteins/suppressors >> Oncoproteins >> Cell survival & death
Cancer >> Invasion/microenvironment >> Apoptosis >> Bcl 2 family
Signal Transduction >> Metabolism >> Mitochondrial
Cell Biology >> Apoptosis >> Intracellular >> Bcl2 Family

Relevance 

The protein encoded by this gene belongs to the Bcl2 protein family. Bcl2 family members form hetero or homodimers and act as anti or pro apoptotic regulators that are involved in a wide variety of cellular activities. The proteins encoded by this gene are located at the outer mitochondrial membrane, and have been shown to regulate outer mitochondrial membrane channel (VDAC) opening. VDAC regulates mitochondrial membrane potential, and thus controls the production of reactive oxygen species and release of cytochrome C by mitochondria, both of which are the potent inducers of cell apoptosis. Two alternatively spliced transcript variants, which encode distinct isoforms, have been reported. The longer isoform acts as an apoptotic inhibitor and the shorter form acts as an apoptotic activator.

Database links 

Raised in 

Rabbit

Clonality 

Monoclonal

Clone number 

E18

Isotype 

IgG

Purity 

IgG fraction

Storage buffer 

Preservative: 0.01% Sodium Azide
Constituents: 40% Glycerol, 0.05% BSA, 0.15M Sodium chloride, 50mM Tris glycine. pH 7.4

Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS

Form 

Liquid

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Notes 

Produced under U.S. Patent No. 5,675,063.

See below for BCL2L1 antibody [E18] images, references, products related to ab32370 and other tools.

BCL2L1 antibody [E18] images:

References for BCL2L1 antibody [E18] (ab32370)

  This product has been used in:

Beilke LD et al. Decreased apoptosis during CAR-mediated hepatoprotection against lithocholic acid-induced liver injury in mice. Toxicol Lett 188:38-44 (2009). WB; Mouse. PubMed: 19433268

If you publish research using ab32370 please let us know so that we can cite the reference on this datasheet.

Customer reviews (feedback) regarding BCL2L1 antibody [E18]

Customer FAQs regarding BCL2L1 antibody [E18]

Protocols for BCL2L1 antibody [E18]

Price and availability of products related to BCL2L1 antibody [E18]

BCL2L1 antibody [E18] - related products: