Anti-BCL2L1 antibody (ab2568)

Western blot - Anti-BCL2L1 antibody (ab2568)

Anti-BCL2L1 antibody (ab2568) at 1/1000 dilution + 25 ug HeLa whole cell lysate at 25 µg

Secondary
Goat anti rabbit (HRP) at 1/4000 dilution

Performed under reducing conditions.

This image was submitted as part of an anonymous Abreview received on the 30th September 2005. We do not have any additional information relating to this image.

Immunoprecipitation - Anti-BCL2L1 antibody (ab2568)

    ab2568 used to immunoprecipitate Bcl xL from whole cell lysate of a human Retinal Pigment Epithelial cell line. 7.5ug of antibody was added per mg of lysate and incubated together with Protein A for 1 hour. 500ug of lysate was added per lane. A peroxidase conjugated goat anti-rabbit IgG was used as the secondary antibody.

Lane 1: Immunoprecipitated material

Lane 2:  Control lane (no antibody)

This image is courtesy of an Abreview submitted by Ingram Iaccarino on 12 April 2006

Western blot - Anti-BCL2L1 antibody (ab2568)

All lanes : Anti-BCL2L1 antibody (ab2568) at 1/1000 dilution

Lane 1 : 20ug whole cell lysate from Retinal Pigment Epithelia
Lane 2 : 20ug whole cell lysate from MDA MB231 cells
Lane 3 : 20ug whole cell lysate from HeLa cells

Secondary
Goat anti-rabbit HRP
developed using the ECL technique

Performed under reducing conditions.

Observed band size : 30 kDa (why is the actual band size different from the predicted?)


Exposure time : 3 minutes

    This image is courtesy of an Abreview submitted by Ingram Iaccarino.

Immunocytochemistry/ Immunofluorescence - Anti-BCL2L1 antibody (ab2568)

ICC/IF image of ab2568 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab2568, 1µg/ml) overnight at +4ºC. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.