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Products:Cell Biology >> Apoptosis >> Intracellular >> Bcl2 Family
Overview
Product name
Anti-BCL2L1 antibody
See all BCL2L1 products (30) ...
Description
Rabbit polyclonal to BCL2L1
Specificity
This antibody detects the 30 kDa Bcl-xL protein. It does not react with other Bcl-2 family members.
Tested applications
IP, WB, ICC/IFmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
Synthetic peptide surrounding amino acid 56 of human Bcl xL. (Peptide available as ab52114.)
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
PBS pH7.2 with 50% glycerol, 1% BSA and 0.02% sodium azide
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Cancer Metabolism >> Response to hypoxia
Cancer >> Tumor biomarkers >> Oncoproteins
Cancer >> Oncoproteins/suppressors >> Oncoproteins >> Cell survival & death
Cancer >> Invasion/microenvironment >> Apoptosis >> Bcl 2 family
Signal Transduction >> Metabolism >> Mitochondrial
Cell Biology >> Apoptosis >> Intracellular >> Bcl2 Family
Applications
Show applications key
Our Abpromise guarantee covers the use of ab2568 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IP: Use a concentration of 20 µg/ml
WB: Use a concentration of 1.0 µg/mlCan be blocked with BCL2L1 peptide (ab52114).
ICC/IF: Use a concentration of 1 µg/ml
Target
Function
Potent inhibitor of cell death. Inhibits activation of caspases (By similarity). Appears to regulate cell death by blocking the voltage-dependent anion channnel (VDAC) by binding to it and preventing the release of the caspase activator, CYC1, from the mitochondrial membrane.
Isoform Bcl-X(S) promotes apoptosis.
Tissue specificity
Bcl-X(S) is expressed at high levels in cells that undergo a high rate of turnover, such as developing lymphocytes. In contrast, Bcl-X(L) is found in tissues containing long-lived postmitotic cells, such as adult brain.
Sequence similarities
Belongs to the Bcl-2 family.
Domain
The BH4 motif is required for anti-apoptotic activity. The BH1 and BH2 motifs are required for both heterodimerization with other Bcl-2 family members and for repression of cell death.
Post-translational
modifications
Proteolytically cleaved by caspases during apoptosis. The cleaved protein, lacking the BH4 motif, has pro-apoptotic activity.
Cellular localization
Mitochondrion membrane. Nucleus membrane. Mitochondrial membranes and perinuclear envelope.
Target information above from: UniProt accessionQ07817
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Apoptosis regulator Bcl X antibody
- Apoptosis regulator Bcl-X antibody
- Apoptosis regulator BclX antibody
see all
Database links
- Entrez Gene: 598 Human
- Entrez Gene: 12048 Mouse
- Entrez Gene: 24888 Rat
- GenBank: CAA80661 Human
- Omim: 600039 Human
- SwissProt: Q07817 Human
- SwissProt: Q5TE63 Human
- SwissProt: Q64373 Mouse
see all
Anti-BCL2L1 antibody images:
Western blot - Anti-BCL2L1 antibody (ab2568)
Anti-BCL2L1 antibody (ab2568) at 1/1000 dilution + 25 ug HeLa whole cell lysate at 25 µg
Secondary
Goat anti rabbit (HRP) at 1/4000 dilution
Performed under reducing conditions.
This image was submitted as part of an anonymous Abreview received on the 30th September 2005. We do not have any additional information relating to this image.
Immunoprecipitation - Anti-BCL2L1 antibody (ab2568)
ab2568 used to immunoprecipitate Bcl xL from whole cell lysate of a human Retinal Pigment Epithelial cell line. 7.5ug of antibody was added per mg of lysate and incubated together with Protein A for 1 hour. 500ug of lysate was added per lane. A peroxidase conjugated goat anti-rabbit IgG was used as the secondary antibody.
Lane 1: Immunoprecipitated material
Lane 2: Control lane (no antibody)
This image is courtesy of an Abreview submitted by Ingram Iaccarino on 12 April 2006
Western blot - Anti-BCL2L1 antibody (ab2568)
All lanes : Anti-BCL2L1 antibody (ab2568) at 1/1000 dilution
Lane 1 : 20ug whole cell lysate from Retinal Pigment Epithelia
Lane 2 : 20ug whole cell lysate from MDA MB231 cells
Lane 3 : 20ug whole cell lysate from HeLa cells
Secondary
Goat anti-rabbit HRP
developed using the ECL technique
Performed under reducing conditions.
Observed band size : 30 kDa (why is the actual band size different from the predicted?)
Exposure time : 3 minutes
This image is courtesy of an Abreview submitted by Ingram Iaccarino.
Immunocytochemistry/ Immunofluorescence - Anti-BCL2L1 antibody (ab2568)
ICC/IF image of ab2568 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab2568, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
References for Anti-BCL2L1 antibody (ab2568)
This product has been referenced in:
- Chen YBet al. Bcl-xL regulates mitochondrial energetics by stabilizing the inner membrane potential. J Cell Biol 195:263-76 (2011).Read more (PubMed: 21987637) »
- Richter-Larrea JAet al. Reversion of epigenetically-mediated BIM silencing overcomes chemoresistance in Burkitt lymphoma. Blood : (2010). WB; Human.Read more (PubMed: 20570860) »
See all 4 publications for this product
Publishing research using ab2568? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"