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Related products
- Secondary antibodies
- Lysates
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Product name
Anti-BCL2L1 antibody
See all BCL2L1 products (29) ...
Description
Rabbit polyclonal to BCL2L1
Tested applications
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Rat, Sheep, Cow, Cat, Dog, Pig
Immunogen
Synthetic peptide corresponding to a region within the N terminal amino acids 2-51 (SQSNRELVVD FLSYKLSQKG YSWSQFSDVE ENRTEAPEGT ESEMETPSAI) of Human BCL2L1 isoform 1 (NP_612815.1).
Positive control
Human fetal thymus lysate
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 2% Sucrose, PBS
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Metabolism >> Types of disease >> Cancer
Metabolism >> Pathways and Processes >> Metabolism processes >> Apoptosis
Metabolism >> Pathways and Processes >> Metabolism processes >> Hypoxia
Metabolism >> Pathways and Processes >> Mitochondrial Metabolism >> Mitochondrial markers
Cancer >> Cancer Metabolism >> Response to hypoxia
Cancer >> Tumor biomarkers >> Oncoproteins
Cancer >> Oncoproteins/suppressors >> Oncoproteins >> Cell survival & death
Cancer >> Invasion/microenvironment >> Apoptosis >> Bcl 2 family
Signal Transduction >> Metabolism >> Mitochondrial
Cell Biology >> Apoptosis >> Intracellular >> Bcl2 Family
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Western blot - BCL2L1 antibody (ab90131)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab90131 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 1 µg/ml. Predicted molecular weight: 26 kDa.Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
Target
Function
Potent inhibitor of cell death. Inhibits activation of caspases (By similarity). Appears to regulate cell death by blocking the voltage-dependent anion channnel (VDAC) by binding to it and preventing the release of the caspase activator, CYC1, from the mitochondrial membrane.
Isoform Bcl-X(S) promotes apoptosis.
Tissue specificity
Bcl-X(S) is expressed at high levels in cells that undergo a high rate of turnover, such as developing lymphocytes. In contrast, Bcl-X(L) is found in tissues containing long-lived postmitotic cells, such as adult brain.
Sequence similarities
Belongs to the Bcl-2 family.
Domain
The BH4 motif is required for anti-apoptotic activity. The BH1 and BH2 motifs are required for both heterodimerization with other Bcl-2 family members and for repression of cell death.
Post-translational
modifications
Proteolytically cleaved by caspases during apoptosis. The cleaved protein, lacking the BH4 motif, has pro-apoptotic activity.
Cellular localization
Mitochondrion membrane. Nucleus membrane. Mitochondrial membranes and perinuclear envelope.
Target information above from: UniProt accessionQ07817
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Apoptosis regulator Bcl X antibody
- Apoptosis regulator Bcl-X antibody
- Apoptosis regulator BclX antibody
see all
Database links
- Entrez Gene: 282152 Cow
- Entrez Gene: 403618 Dog
- Entrez Gene: 598 Human
- Entrez Gene: 12048 Mouse
- Entrez Gene: 397536 Pig
- Entrez Gene: 24888 Rat
- GenBank: CAA80661 Human
- Omim: 600039 Human
see all
Anti-BCL2L1 antibody images:
Western blot - BCL2L1 antibody (ab90131)
Anti-BCL2L1 antibody (ab90131) at 1 µg/ml (in 5% skim milk / PBS buffer) + human fetal thymus lysate at 10 µg
Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size : 26 kDa
References for Anti-BCL2L1 antibody (ab90131)
ab90131 has not yet been referenced specifically in any publications.
Publishing research using ab90131? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"