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- Secondary antibodies
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Product name
Anti-BCL2L1 antibody, prediluted
See all BCL2L1 products (29) ...
Description
Rabbit polyclonal to BCL2L1, prediluted
Tested applications
WB, IHC-Pmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Rat
Immunogen
Synthetic peptide (unfortunately, the amino acid sequence is considered to be commercially sensitive) (Human) (N terminal).
Positive control
Colon carcinoma.
Properties
Form
Prediluted
Storage instructions
Store at +4°C.
Storage buffer
Preservative: 0.1% Sodium Azide
Constituents: 1% BSA, 50mM Tris, pH 7.6
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Metabolism >> Types of disease >> Cancer
Metabolism >> Pathways and Processes >> Metabolism processes >> Apoptosis
Metabolism >> Pathways and Processes >> Metabolism processes >> Hypoxia
Metabolism >> Pathways and Processes >> Mitochondrial Metabolism >> Mitochondrial markers
Cancer >> Cancer Metabolism >> Response to hypoxia
Cancer >> Tumor biomarkers >> Oncoproteins
Cancer >> Oncoproteins/suppressors >> Oncoproteins >> Cell survival & death
Cancer >> Invasion/microenvironment >> Apoptosis >> Bcl 2 family
Signal Transduction >> Metabolism >> Mitochondrial
Cell Biology >> Apoptosis >> Intracellular >> Bcl2 Family
Applications
Show applications key
Our Abpromise guarantee covers the use of ab15276 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use at an assay dependent dilution. Predicted molecular weight: 26 kDa.
IHC-P: Use at an assay dependent dilution. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.Suggested working dilution of 1/4 (see PMID: 16299246). Heat mediated antigen retrieval may be required before commencing with IHC staining protocol.
Target
Function
Potent inhibitor of cell death. Inhibits activation of caspases (By similarity). Appears to regulate cell death by blocking the voltage-dependent anion channnel (VDAC) by binding to it and preventing the release of the caspase activator, CYC1, from the mitochondrial membrane.
Isoform Bcl-X(S) promotes apoptosis.
Tissue specificity
Bcl-X(S) is expressed at high levels in cells that undergo a high rate of turnover, such as developing lymphocytes. In contrast, Bcl-X(L) is found in tissues containing long-lived postmitotic cells, such as adult brain.
Sequence similarities
Belongs to the Bcl-2 family.
Domain
The BH4 motif is required for anti-apoptotic activity. The BH1 and BH2 motifs are required for both heterodimerization with other Bcl-2 family members and for repression of cell death.
Post-translational
modifications
Proteolytically cleaved by caspases during apoptosis. The cleaved protein, lacking the BH4 motif, has pro-apoptotic activity.
Cellular localization
Mitochondrion membrane. Nucleus membrane. Mitochondrial membranes and perinuclear envelope.
Target information above from: UniProt accessionQ07817
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Apoptosis regulator Bcl X antibody
- Apoptosis regulator Bcl-X antibody
- Apoptosis regulator BclX antibody
see all
Database links
- Entrez Gene: 598 Human
- Entrez Gene: 12048 Mouse
- Entrez Gene: 24888 Rat
- GenBank: CAA80661 Human
- Omim: 600039 Human
- SwissProt: Q5TE63 Human
- SwissProt: Q07817 Human
- SwissProt: Q64373 Mouse
see all
Anti-BCL2L1 antibody, prediluted images:
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - BCL2L1 antibody, prediluted (ab15276)
ab15276 staining human Bcl xl in human Colon Carcinoma by Immunohistochemistry (FFPE-sections).
Western blot - BCL2L1 antibody, prediluted (ab15276)
Anti-BCL2L1 antibody, prediluted (ab15276) at 1/100 dilution + 293T cell lysate
Predicted band size : 26 kDa
Observed band size : 28 kDa (why is the actual band size different from the predicted?)
The dilution stated here refers to the dilution used for a non-prediluted version of this product.
References for Anti-BCL2L1 antibody, prediluted (ab15276)
This product has been referenced in:
- Yip KW et al. Combination bcl-2 antisense and radiation therapy for nasopharyngeal cancer. Clin Cancer Res 11:8131-44 (2005).Read more (PubMed: 16299246) »
See 1 publication for this product
Publishing research using ab15276? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"