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Overview

  • Product nameAnti-BCMA antibodySee all BCMA primary antibodies ...
  • Description
    Rabbit polyclonal to BCMA
  • Tested applicationsWB, ELISA, IHC-P more details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide, conjugated to KLH, from the C terminal region of Human BCMA.

  • Positive controlHuman spleen cells; Human colon tissue; Human spleen tissue lysate; K562 cell lysate; U937 cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab115315 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB WB: Use a concentration of 2 - 5 µg/ml. Predicted molecular weight: 20 kDa.
ELISA ELISA: Use at an assay dependent dilution.
IHC-P IHC-P: Use a concentration of 3 - 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • FunctionReceptor for TNFSF13B/BLyS/BAFF and TNFSF13/APRIL. Promotes B-cell survival and plays a role in the regulation of humoral immunity. Activates NF-kappa-B and JNK.
  • Tissue specificityExpressed in mature B-cells, but not in T-cells or monocytes.
  • Involvement in diseaseNote=A chromosomal aberration involving TNFRSF17 is found in a form of T-cell acute lymphoblastic leukemia (T-ALL). Translocation t(4;16)(q26;p13) with IL2.
  • Sequence similaritiesContains 1 TNFR-Cys repeat.
  • Cellular localizationCell membrane. Endomembrane system. Perinuclear Golgi-like structures.

    • Target information above from: UniProt accession Q02223 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
    • Alternative names
        B cell maturation antigen antibodyB cell maturation factor antibodyB cell maturation protein antibody
        BCM antibodyBCMA antibodyCD269 antibodyCD269 antigen antibodyTNFRSF17 antibodyTumor necrosis factor receptor superfamily member 17 antibody
      see all

    Anti-BCMA antibody images

    • Western blot - Anti-BCMA antibody (ab115315)
      Western blot - Anti-BCMA antibody (ab115315)
      All lanes : Anti-BCMA antibody (ab115315) at 5 µg/ml

      Lane 1 : Human spleen lysate
      Lane 2 : K562 cell lysate
      Lane 3 : U937 cell lysate


      Predicted band size : 20 kDa
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BCMA antibody (ab115315)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BCMA antibody (ab115315)
      ab115315 at 3ug/ml staining BCMA in Human colon tissue by immunohistochemistry (FFPE). After primary incubation slides were incubated with biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BCMA antibody (ab115315)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BCMA antibody (ab115315)
      ab115315 at 10ug/ml staining BCMA in Human spleen cells by immunohistochemistry (FFPE).

    References for Anti-BCMA antibody (ab115315)

    ab115315 has not yet been referenced specifically in any publications.

    Publishing research using ab115315 ? Please let us know so that we can cite the reference in this datasheet

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    Low level of specific staining in human spleen and lymphoma FFPE tissue.

    Thank you very much for your call yesterday and for letting us know about the trouble with this antibody.

    As we discussed, you can send the protocol details and an image in a reply to this email, and I'll be happy to see if there are any changes...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"