BCRP/ABCG2 antibody [BXP-21] - Hematopoietic/Neural Stem Cell Marker (ab3380)

Product Name 

BCRP/ABCG2 antibody [BXP-21] - Hematopoietic/Neural Stem Cell Marker

Product type 

Primary antibodies

Description 

Mouse monoclonal [BXP-21] to BCRP/ABCG2 - Hematopoietic/Neural Stem Cell Marker

Immunogen 

Fusion protein composed of E. Coli maltose binding protein and BCRP peptide [126 amino acids, 271-396 AA of BCRP, GeneBank accession #AF098951].

Reacts with 
(species key)

Hu

Specificity 

This antibody does not react with the human MDR1, MRP1 and MRP2 gene products.

Tested applications 
(see key)

ICC, IHC-Fr, IHC-P, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews (Read all Abreviews)

Application notes 
(see key)

Recommended dilutions
ICC: 1/20 - 1/50. (Acetone fixed cells)
IHC-P: 1/20 - 1/40. (Pre treatment with citrate buffer pH 6.0 is required)
IHC-Fr: 1/20 - 1/40.
WB: Use at an assay dependent dilution. Predicted molecular weight: 72.3 kDa.

Not tested in other applications.

Positive control 
(see definition)

Caco Cell lysate (see second reference cited).

Cellular localization 

Plasma membrane, integral membrane protein

Research areas 

Cancer >> Drug resistance >> MRP-related proteins
Signal Transduction >> Metabolism >> Drug metabolism
Stem Cells >> Hematopoietic Progenitors >> Surface Molecules
Stem Cells >> Neural Stem Cells >> Surface Molecules
Signal Transduction >> Metabolism >> Plasma Membrane >> ATPases
Tags & Cell Markers >> Cell Type Markers >> Tumor Associated

Relevance 

An ABC transporter. Allows efflux of Hoechst dye, a property that has been used to separate bone marrow side population cells, which express BCRP/ABCG2. Appears to play a major role in the multidrug resistance phenotype of a specific MCF-7 breast cancer cell line. When overexpressed, the transfected cells become resistant to mitoxantrone, daunorubicin and doxorubicin, display diminished intracellular accumulation of daunorubicin, and manifest an ATP-dependent increase in the efflux of rhodamine 123.

Breast Cancer Resistance Protein (BCRP) is a 70 kDa ATP-Binding Cassette membrane transport protein involved in multidrug resistance. BCRP may be over-expressed in cancer cell lines selected with doxorubicin / verapamil, topotecan or mitoxantrone.

Database links 

Raised in 

Mouse

Clonality 

Monoclonal

Clone number 

BXP-21

Isotype 

IgG2a

Purity 

Tissue culture supernatant

Storage buffer 

Preservative: 0.1% Sodium Azide
Constituents: 1% BSA

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Concentration 

Concentration not determined - why is this?

Storage instructions 

Store at +4°C.

References for BCRP/ABCG2 antibody [BXP-21] - Hematopoietic/Neural Stem Cell Marker (ab3380)

  This product has been used in: (two most recent references)

Zhang P et al. Side population in oral squamous cell carcinoma possesses tumor stem cell phenotypes. Cancer Lett 277:227-34 (2009). ICC/IF. PubMed: 19185988

Mannermaa E et al. Efflux protein expression in human retinal pigment epithelium cell lines. Pharm Res 26:1785-91 (2009). WB; Human. PubMed: 19384462

See all 9 publication references for this product.


If you publish research using ab3380 please let us know so that we can cite the reference on this datasheet.

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