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Anti-BCRP/ABCG2 antibody [BXP-21] - Hematopoietic/Neural Stem Cell Marker (ab3380)

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Overview

Product name

Anti-BCRP/ABCG2 antibody [BXP-21] - Hematopoietic/Neural Stem Cell Marker
See all BCRP/ABCG2 products (10) ...

Description

Mouse monoclonal [BXP-21] to BCRP/ABCG2 - Hematopoietic/Neural Stem Cell Marker

Specificity

This antibody does not react with the human MDR1, MRP1 and MRP2 gene products.

Tested applications

ICC, IHC-P, IHC-Fr, WB, ICC/IFmore details

Cross reactivity

Reacts with

Human

Immunogen

Fusion protein composed of E. Coli maltose binding protein and BCRP peptide [126 amino acids, 271-396 AA of BCRP, GeneBank accession #AF098951].

Positive control

Caco Cell lysate (see second reference cited).

Properties

Form

Liquid

Storage instructions

Store at +4°C.

Storage buffer

Preservative: 0.1% Sodium Azide
Constituents: 1% BSA

Purity

Tissue culture supernatant

Primary antibody notes

Breast Cancer Resistance Protein (BCRP) is a 70 kDa ATP-Binding Cassette membrane transport protein involved in multidrug resistance. BCRP may be over-expressed in cancer cell lines selected with doxorubicin / verapamil, topotecan or mitoxantrone.

Clonality

Monoclonal

Clone number

BXP-21

Isotype

IgG2a

Research areas

Cancer >> Drug resistance >> MRP-related proteins
Signal Transduction >> Metabolism >> Drug metabolism
Stem Cells >> Hematopoietic Progenitors >> Surface Molecules
Stem Cells >> Neural Stem Cells >> Surface Molecules
Signal Transduction >> Metabolism >> Plasma Membrane >> ATPases
Tags & Cell Markers >> Cell Type Markers >> Tumor Associated

  • Immunocytochemistry/ Immunofluorescence - BCRP/ABCG2 antibody [BXP-21] - Hematopoietic/Neural Stem Cell Marker (ab3380)Immunocytochemistry/ Immunofluorescence - BCRP/ABCG2 antibody [BXP-21] - Hematopoietic/Neural Stem Cell Marker (ab3380) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab3380 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Target

Function

Xenobiotic transporter that may play an important role in the exclusion of xenobiotics from the brain. May be involved in brain-to-blood efflux. Appears to play a major role in the multidrug resistance phenotype of several cancer cell lines. When overexpressed, the transfected cells become resistant to mitoxantrone, daunorubicin and doxorubicin, display diminished intracellular accumulation of daunorubicin, and manifest an ATP-dependent increase in the efflux of rhodamine 123.

Tissue specificity

Highly expressed in placenta. Low expression in small intestine, liver and colon.

Sequence similarities

Belongs to the ABC transporter superfamily. ABCG family. Eye pigment precursor importer (TC 3.A.1.204) subfamily.
Contains 1 ABC transmembrane type-2 domain.
Contains 1 ABC transporter domain.

Post-translational
modifications

Glycosylation-deficient ABCG2 is normally expressed and functional.

Cellular localization

Cell membrane.

Target information above from: UniProt accessionQ9UNQ0 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • ABC15 antibody
  • ABCG 2 antibody
  • ABCG2 antibody
  • ABCG2_HUMAN antibody
  • ABCP antibody
  • ATP-binding cassette sub-family G member 2 antibody
  • BCRP1 antibody
  • BMDP antibody
  • Breast cancer resistance protein antibody
  • CD338 antibody
  • CDw338 antibody
  • CDw338 antigen antibody
  • EST157481 antibody
  • MGC102821 antibody
  • Mitoxantrone resistance associated protein antibody
  • Mitoxantrone resistance-associated protein antibody
  • MRX antibody
  • MXR antibody
  • MXR1 antibody
  • Placenta specific ATP binding cassette transporter antibody
  • Placenta-specific ATP-binding cassette transporter antibody
see all

Database links

Anti-BCRP/ABCG2 antibody [BXP-21] - Hematopoietic/Neural Stem Cell Marker images:

  Immunocytochemistry/ Immunofluorescence - BCRP/ABCG2 antibody [BXP-21] - Hematopoietic/Neural Stem Cell Marker (ab3380)

Immunocytochemistry/ Immunofluorescence - BCRP/ABCG2 antibody [BXP-21] - Hematopoietic/Neural Stem Cell Marker (ab3380)

ab3380 staining BCRP/ABCG2 in MCF7 human breast cancer cells by Immunocytochemistry/ Immunofluorescence.

Cells were fixed in paraformaldehyde and permeabilized using 0.1% Triton X-100. Samples were then blocked using 10% serum for 2 hours at 25°C, then incubated with ab3380 at a 1/50 dilution for 12 hours at 4°C. A rabbit anti-mouse polyclonal conjugated to Alexa Fluor 594 was used as the secondary at a 1/50 dilution.

Image courtesy of Dr Gerald Davies by Abreview.

See Abreview

References for Anti-BCRP/ABCG2 antibody [BXP-21] - Hematopoietic/Neural Stem Cell Marker (ab3380)

This product has been referenced in:

  • Kinsella Pet al. Imatinib and docetaxel in combination can effectively inhibit glioma invasion in an in vitro 3D invasion assay. J Neurooncol 101:189-98 (2011). WB; Human.Read more (PubMed: 20512610) »
  • Hardwick RNet al. Variations in ABC-Transporter Regulation during the Progression of Human Nonalcoholic Fatty Liver Disease. Drug Metab Dispos : (2011). WB, IHC-P; Human.Read more (PubMed: 21878559) »

See all 15 publications for this product

Publishing research using ab3380? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"