![Western blot - BCRP/ABCG2 antibody [BXP-53] (ab24115) image](#BCRPABCG2-Primary-antibodies-ab24115-2.jpg)
 image](#BCRPABCG2-Primary-antibodies-ab24115-3.jpg)
![Immunocytochemistry/ Immunofluorescence - BCRP/ABCG2 antibody [BXP-53] (ab24115) image](#BCRPABCG2-Primary-antibodies-ab24115-6.jpg)
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Products:Tags & Cell Markers >> Cell Type Markers >> Tumor Associated
Overview
Product name
Anti-BCRP/ABCG2 antibody [BXP-53]
See all BCRP/ABCG2 products (10) ...
Description
Rat monoclonal [BXP-53] to BCRP/ABCG2
Tested applications
ICC, IHC-Fr, WB, Flow Cyt, IHC-P, ICC/IFmore details
Cross reactivity
Reacts with
Mouse, Human
Immunogen
Fusion protein containing the E. coli maltose binding protein and a fragment corresponding to amino acids 221-394 of Mouse BCRP/ABCG2
Epitope
Reacts with an internal epitope of BCRP/ABCG2.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.1% Sodium Azide
Constituents: 0.7% BSA, Tissue culture supernatant
Purity
Tissue culture supernatant
Clonality
Monoclonal
Clone number
BXP-53
Isotype
IgG2a
Research areas
Cancer >> Drug resistance >> MRP-related proteins
Signal Transduction >> Metabolism >> Drug metabolism
Stem Cells >> Hematopoietic Progenitors >> Surface Molecules
Stem Cells >> Neural Stem Cells >> Surface Molecules
Signal Transduction >> Metabolism >> Plasma Membrane >> ATPases
Tags & Cell Markers >> Cell Type Markers >> Tumor Associated
Applications
Show applications key
Our Abpromise guarantee covers the use of ab24115 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC: 1/20 - 1/50.(Acetone fixed cytospin.)
IHC-Fr: 1/20(Acetone fixed. Use biotin-streptavidin amplification.)
WB: 1/20 - 1/50.Detects a band of approximately 75 kDa (predicted molecular weight: 72 kDa).
Flow Cyt: Use 1-2µg for 106 cells. ((1/10 dilution on bone marrow and kidney tissue).)
IHC-P: Use at an assay dependent dilution. (PubMed: 20472681)
ICC/IF: Use at an assay dependent dilution.
Target
Function
Xenobiotic transporter that may play an important role in the exclusion of xenobiotics from the brain. May be involved in brain-to-blood efflux. Appears to play a major role in the multidrug resistance phenotype of several cancer cell lines. When overexpressed, the transfected cells become resistant to mitoxantrone, daunorubicin and doxorubicin, display diminished intracellular accumulation of daunorubicin, and manifest an ATP-dependent increase in the efflux of rhodamine 123.
Tissue specificity
Highly expressed in placenta. Low expression in small intestine, liver and colon.
Sequence similarities
Belongs to the ABC transporter superfamily. ABCG family. Eye pigment precursor importer (TC 3.A.1.204) subfamily.
Contains 1 ABC transmembrane type-2 domain.
Contains 1 ABC transporter domain.
Post-translational
modifications
Glycosylation-deficient ABCG2 is normally expressed and functional.
Cellular localization
Cell membrane.
Target information above from: UniProt accessionQ9UNQ0
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- ABC15 antibody
- ABCG 2 antibody
- ABCG2 antibody
see all
Database links
- Entrez Gene: 9429 Human
- Entrez Gene: 26357 Mouse
- Omim: 603756 Human
- SwissProt: Q9UNQ0 Human
- SwissProt: Q7TMS5 Mouse
- Unigene: 480218 Human
- Unigene: 333096 Mouse
Anti-BCRP/ABCG2 antibody [BXP-53] images:
Western blot - BCRP/ABCG2 antibody [BXP-53] (ab24115)
![Western blot - BCRP/ABCG2 antibody [BXP-53] (ab24115)](/ps/datasheet/images/24/ab24115/BCRPABCG2-Primary-antibodies-ab24115-2.jpg)
All lanes : Anti-BCRP/ABCG2 antibody [BXP-53] (ab24115) at 1 µg/ml
Lane 1 : Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate
Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Peroxidase Conjugated AffiniPure Rabbit Anti-Rat IgG (H+L) at 1/10000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 72 kDa
Observed band size : 75 kDa (why is the actual band size different from the predicted?)
Additional bands at : 37 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 20 minutes
BCRP/ABCG2 contains a potential glycosylation site (SwissProt) which may explain its migration at a higher molecular weight than predicted.
Flow Cytometry-BCRP/ABCG2 antibody [BXP-53](ab24115)
](/ps/datasheet/images/24/ab24115/BCRPABCG2-Primary-antibodies-ab24115-3.jpg)
Overlay histogram showing HEK293 cells stained with ab24115 (red line). The cells were fixed with methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab24115, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rat IgG (Fc) (ab96971) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rat IgG2a [aRTK2758] (ab18450, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a significantly decreased signal in HEK293 cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.
Immunocytochemistry/ Immunofluorescence - BCRP/ABCG2 antibody [BXP-53] (ab24115)
![Immunocytochemistry/ Immunofluorescence - BCRP/ABCG2 antibody [BXP-53] (ab24115)](/ps/datasheet/images/24/ab24115/BCRPABCG2-Primary-antibodies-ab24115-6.jpg)
ab24115 staining BCRP/ABCG2 in murine mesenchymal stem cells by Immunocytochemistry/ Immunofluorescence. The cells were fixed in formaldehyde, permeabilised in 0.1% Triton X and then blocked using 1% serum for 1 hour at 25°C. Samples were then incubated with primary antibody at 1/25 for 15 hours at 25°C. The secondary antibody used was a donkey anti-rat IgG conjugated to an Alexa Fluor® used at a 1/100 dilution.
Image courtesy of an anonymous Abreview.
References for Anti-BCRP/ABCG2 antibody [BXP-53] (ab24115)
This product has been referenced in:
- Zander SAet al. Sensitivity and acquired resistance of BRCA1;p53-deficient mouse mammary tumors to the topoisomerase I inhibitor topotecan. Cancer Res 70:1700-10 (2010). IHC; Mouse.Read more (PubMed: 20145144) »
- de Vries NAet al. Rapid and robust transgenic high-grade glioma mouse models for therapy intervention studies. Clin Cancer Res 16:3431-41 (2010). IHC-P; Mouse.Read more (PubMed: 20472681) »
See all 7 publications for this product
Publishing research using ab24115? Please let us know so that we can cite the reference in this datasheet
![BCRP/ABCG2 antibody [BXP-53] for Immunocytochemistry/ Immunofluorescence in Mouse (24115)](/ps/datasheet/images/24/ab24115/BCRPABCG2-Primary-antibodies-ab24115-5.GIF)
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"