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Have these antibodies been tested in FACS? |
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ANSWER: |
Thank you for your enquiry. The antibodies were tested by FACS on bone marrow and kidney tissue, but we have no data or a publication available. We know that one customer has compared the two and found that ab24115 was more sensitive than ab24114. He used a dilution of 1:10. The antibodies are not suitable on viable, unfixed cells. I hope this information helps, please do not hesitate to contact us if you need any more advice or information. |
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Do I need to permeabilise my cells in FACS, i.e. is the epitope internal or extracellular? |
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ANSWER: |
The immunogen was raised against amino acids 221-394 of the Mouse Protein and according to Swiss Prot the amino acids 1-393 are cytoplasmic so the antibody recognizes the intracellular portion of the protein. We expect the FACS protocol to require permeabilization of the cells, I hope this information helps,
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"