BCRP/ABCG2 antibody [BXP-9] (ab24114)

Product Name 

BCRP/ABCG2 antibody [BXP-9]

Product type 

Primary antibodies

Description 

Rat monoclonal [BXP-9] to BCRP/ABCG2

Immunogen 

A fusion protein containing the E. coli maltose binding protein and a fragment of the mouse bcrp protein corresponding to amino acids 221-394.

Reacts with 

Ms

Does not react with 
(species key)

Hu

Tested applications 
(see key)

Flow Cyt, ICC, IHC-Fr, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
Flow Cyt: Use at an assay dependent dilution. The antibody was tested in FACS at a 1/10 dilution on on bone marrow and kidney tissue.
ICC: 1/20 - 1/50 (acetone fixed cytospin preparations).
IHC-Fr: 1/20. Fix with acetone, use biotin-streptavidin amplification.
WB: 1/20 - 1/50. Predicted molecular weight: 70 kDa.

Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

bone marrow and kidney tissue

Cellular localization 

Plasma membrane, integral membrane protein

Research areas 

Cancer >> Drug resistance >> MRP-related proteins
Signal Transduction >> Metabolism >> Drug metabolism
Stem Cells >> Hematopoietic Progenitors >> Surface Molecules
Stem Cells >> Neural Stem Cells >> Surface Molecules
Signal Transduction >> Metabolism >> Plasma Membrane >> ATPases
Tags & Cell Markers >> Cell Type Markers >> Tumor Associated

Relevance 

An ABC transporter. Allows efflux of Hoechst dye, a property that has been used to separate bone marrow side population cells, which express BCRP/ABCG2. Appears to play a major role in the multidrug resistance phenotype of a specific MCF-7 breast cancer cell line. When overexpressed, the transfected cells become resistant to mitoxantrone, daunorubicin and doxorubicin, display diminished intracellular accumulation of daunorubicin, and manifest an ATP-dependent increase in the efflux of rhodamine 123.

Database links 

Raised in 

Rat

Clonality 

Monoclonal

Clone number 

BXP-9

Isotype 

IgG1

Purity 

Tissue culture supernatant

Storage buffer 

Preservative: 0.1% Sodium Azide
Constituents: 0.7% BSA, Tissue culture supernatant (serum free)

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Concentration 

0.250 mg/ml

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

References for BCRP/ABCG2 antibody [BXP-9] (ab24114)

  This product has been used in: (two most recent references)

Hoshi N et al. Side population cells in the mouse thyroid exhibit stem/progenitor cell-like characteristics. Endocrinology 148:4251-8 (2007). PubMed: 17584961

Jang YY & Sharkis SJ A low level of reactive oxygen species selects for primitive hematopoietic stem cells that may reside in the low-oxygenic niche. Blood 110:3056-63 (2007). Flow Cyt; Mouse. PubMed: 17595331

See all 3 publication references for this product.


If you publish research using ab24114 please let us know so that we can cite the reference on this datasheet.

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