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Overview

  • Product nameAnti-BCRP/ABCG2 antibody [BXP-9]See all BCRP/ABCG2 primary antibodies ...
  • Description
    Rat monoclonal [BXP-9] to BCRP/ABCG2
  • Tested applicationsIHC-P, ICC, IHC-Fr, WB, Flow Cyt more details
  • Species reactivity
    Reacts with: Mouse

    Does not react with

    Human
  • Immunogen

    A fusion protein containing the E. coli maltose binding protein and a fragment of the mouse bcrp protein corresponding to amino acids 221-394.

  • Positive controlbone marrow and kidney tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab24114 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
IHC-P IHC-P: Use at an assay dependent dilution. PubMed: 20133811
ICC ICC: 1/20 - 1/50. Acetone fixed cytospin preparations
IHC-Fr IHC-Fr: 1/20. Fix with acetone and use biotin-streptavidin amplification.
WB WB: 1/20 - 1/50. Predicted molecular weight: 70 kDa.
Flow Cyt Flow Cyt: Use at an assay dependent dilution.

Target

  • FunctionXenobiotic transporter that may play an important role in the exclusion of xenobiotics from the brain. May be involved in brain-to-blood efflux. Appears to play a major role in the multidrug resistance phenotype of several cancer cell lines. When overexpressed, the transfected cells become resistant to mitoxantrone, daunorubicin and doxorubicin, display diminished intracellular accumulation of daunorubicin, and manifest an ATP-dependent increase in the efflux of rhodamine 123.
  • Tissue specificityHighly expressed in placenta. Low expression in small intestine, liver and colon.
  • Sequence similaritiesBelongs to the ABC transporter superfamily. ABCG family. Eye pigment precursor importer (TC 3.A.1.204) subfamily.
    Contains 1 ABC transmembrane type-2 domain.
    Contains 1 ABC transporter domain.
  • Post-translational
    modifications    Glycosylation-deficient ABCG2 is normally expressed and functional.
  • Cellular localizationCell membrane.

    • Target information above from: UniProt accession Q9UNQ0 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
    • Alternative names
        ABC transporter antibodyABC15 antibodyABCG 2 antibody
        ABCG2 antibodyABCG2_HUMAN antibodyABCP antibodyATP binding cassette sub family G (WHITE) member 2 antibodyATP binding cassette transporter G2 antibodyATP-binding cassette sub-family G member 2 antibodyBCRP antibodyBCRP1 antibodyBMDP antibodyBreast cancer resistance protein antibodyCD338 antibodyCDw338 antibodyCDw338 antigen antibodyEST157481 antibodyGOUT1 antibodyMGC102821 antibodyMitoxantrone resistance associated protein antibodyMitoxantrone resistance-associated protein antibodyMRX antibodyMulti drug resistance efflux transport ATP binding cassette sub family G (WHITE) member 2 antibodyMXR antibodyMXR1 antibodyPlacenta specific ATP binding cassette transporter antibodyPlacenta specific MDR protein antibodyPlacenta-specific ATP-binding cassette transporter antibodyUAQTL1 antibody
      see all

    References for Anti-BCRP/ABCG2 antibody [BXP-9] (ab24114)

    This product has been referenced in:
    • Li F  et al. Generation of a novel transgenic mouse model for bioluminescent monitoring of survivin gene activity in vivo at various pathophysiological processes: survivin expression overlaps with stem cell markers. Am J Pathol 176:1629-38 (2010). IHC-P ; Mouse . Read more (PubMed: 20133811) »
    • Hoshi N  et al. Side population cells in the mouse thyroid exhibit stem/progenitor cell-like characteristics. Endocrinology 148:4251-8 (2007). Read more (PubMed: 17584961) »

    See all 3 Publications for this product

    Publishing research using ab24114 ? Please let us know so that we can cite the reference in this datasheet

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    Submit an Abreview for ab24114 Submit a Question for ab24114

    Displaying 1 - 3 of 3 results for Abreviews and Q&A

    10
    Votes: Highest First

    I have seen no staining of mouse lung in IHC-P; primary used at 1:20 - 1:100; secondary is OK; used no retrieval; contacted authors who published with this antibody/application and they said antibody was tempermental;

    I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement for one vial of ab24115.

    To check the status of the order please contact our ...

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    Have these antibodies been tested in FACS?

    Thank you for your enquiry. The antibodies were tested by FACS on bone marrow and kidney tissue, but we have no data or a publication available. We know that one customer has compared the two and found that ab24115 was more sensitive than ab24114....

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    Do I need to permeabilise my cells in FACS, i.e. is the epitope internal or extracellular?

    The immunogen was raised against amino acids 221-394 of the Mouse Protein and according to Swiss Prot the amino acids 1-393 are cytoplasmic so the antibody recognizes the intracellular portion of the protein. We expect the FACS protocol to require permeab...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"