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Anti-BCRP/ABCG2 antibody [BXP-9] (ab24114)

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Overview

Product name

Anti-BCRP/ABCG2 antibody [BXP-9]
See all BCRP/ABCG2 products (10) ...

Description

Rat monoclonal [BXP-9] to BCRP/ABCG2

Tested applications

IHC-P, ICC, IHC-Fr, WB, Flow Cytmore details

Cross reactivity

Reacts with

Mouse

Does not react with

Human

Immunogen

A fusion protein containing the E. coli maltose binding protein and a fragment of the mouse bcrp protein corresponding to amino acids 221-394.

Positive control

bone marrow and kidney tissue

Properties

Form

Liquid

Storage instructions

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: 0.1% Sodium Azide
Constituents: 0.7% BSA, Tissue culture supernatant (serum free)

Purity

Tissue culture supernatant

Clonality

Monoclonal

Clone number

BXP-9

Isotype

IgG1

Research areas

Cancer >> Drug resistance >> MRP-related proteins
Signal Transduction >> Metabolism >> Drug metabolism
Stem Cells >> Hematopoietic Progenitors >> Surface Molecules
Stem Cells >> Neural Stem Cells >> Surface Molecules
Signal Transduction >> Metabolism >> Plasma Membrane >> ATPases
Tags & Cell Markers >> Cell Type Markers >> Tumor Associated

Applications

Show applications key

Our Abpromise guarantee covers the use of ab24114 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • ShowHide

    IHC-P

     IHC-P: Use at an assay d...Read more →

    IHC-P: Use at an assay dependent dilution. (PubMed: 20133811)

  • ShowHide

    ICC

     ICC: 1/20 - 1/50.(Aceton...Read more →

    ICC: 1/20 - 1/50.(Acetone fixed cytospin preparations)

  • ShowHide

    IHC-Fr

     IHC-Fr: 1/20(Fix with ac...Read more →

    IHC-Fr: 1/20(Fix with acetone and use biotin-streptavidin amplification. )

  • ShowHide

    WB

     WB: 1/20 - 1/50.Predicte...Read more →

    WB: 1/20 - 1/50.Predicted molecular weight: 70 kDa.

  • ShowHide

    Flow Cyt

     Flow Cyt: Use at an assa...Read more →

    Flow Cyt: Use at an assay dependent dilution.

Target

Function

Xenobiotic transporter that may play an important role in the exclusion of xenobiotics from the brain. May be involved in brain-to-blood efflux. Appears to play a major role in the multidrug resistance phenotype of several cancer cell lines. When overexpressed, the transfected cells become resistant to mitoxantrone, daunorubicin and doxorubicin, display diminished intracellular accumulation of daunorubicin, and manifest an ATP-dependent increase in the efflux of rhodamine 123.

Tissue specificity

Highly expressed in placenta. Low expression in small intestine, liver and colon.

Sequence similarities

Belongs to the ABC transporter superfamily. ABCG family. Eye pigment precursor importer (TC 3.A.1.204) subfamily.
Contains 1 ABC transmembrane type-2 domain.
Contains 1 ABC transporter domain.

Post-translational
modifications

Glycosylation-deficient ABCG2 is normally expressed and functional.

Cellular localization

Cell membrane.

Target information above from: UniProt accessionQ9UNQ0 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • ABC15 antibody
  • ABCG 2 antibody
  • ABCG2 antibody
  • ABCG2_HUMAN antibody
  • ABCP antibody
  • ATP-binding cassette sub-family G member 2 antibody
  • BCRP1 antibody
  • BMDP antibody
  • Breast cancer resistance protein antibody
  • CD338 antibody
  • CDw338 antibody
  • CDw338 antigen antibody
  • EST157481 antibody
  • MGC102821 antibody
  • Mitoxantrone resistance associated protein antibody
  • Mitoxantrone resistance-associated protein antibody
  • MRX antibody
  • MXR antibody
  • MXR1 antibody
  • Placenta specific ATP binding cassette transporter antibody
  • Placenta-specific ATP-binding cassette transporter antibody
see all

Database links

Protocols

  • Recommended FACS protocol for ab24114 and ab24115

    • References for Anti-BCRP/ABCG2 antibody [BXP-9] (ab24114)

    This product has been referenced in:

    • Li Fet al. Generation of a novel transgenic mouse model for bioluminescent monitoring of survivin gene activity in vivo at various pathophysiological processes: survivin expression overlaps with stem cell markers. Am J Pathol 176:1629-38 (2010). IHC-P; Mouse.Read more (PubMed: 20133811) »
    • Hoshi Net al. Side population cells in the mouse thyroid exhibit stem/progenitor cell-like characteristics. Endocrinology 148:4251-8 (2007).Read more (PubMed: 17584961) »

    See all 4 publications for this product

    Publishing research using ab24114? Please let us know so that we can cite the reference in this datasheet

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"