Directly conjugated antibodies for immunofluorescence

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Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab75120 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 40 kDa).Can be blocked with Human BDKRB1 peptide (ab75119).

Target

  • FunctionThis is a receptor for bradykinin. Could be a factor in chronic pain and inflammation.
  • Sequence similaritiesBelongs to the G-protein coupled receptor 1 family. Bradykinin receptor subfamily. BDKRB1 sub-subfamily.
  • Cellular localizationCell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • B1 bradykinin receptor antibody
    • B1BKR antibody
    • B1R antibody
    • BDKR B1 antibody
    • BDKRB 1 antibody
    • BDKRB1 antibody
    • BK 1 receptor antibody
    • BK-1 receptor antibody
    • BKB1R antibody
    • BKR 1 antibody
    • BKR1 antibody
    • BKRB1_HUMAN antibody
    • BRADY B1 antibody
    • BRADYB1 antibody
    • Bradykinin B1 receptor antibody
    • Bradykinin receptor 1 antibody
    • Bradykinin receptor B1 antibody
    see all

Anti-BDKRB1 antibody images

  • All lanes : Anti-BDKRB1 antibody (ab75120) at 1 µg/ml

    Lane 1 : U2OS (Human osteosarcoma cell line) Whole Cell Lysate
    Lane 2 : WERI (Human Retinoblastoma) Whole Cell Lysate
    Lane 3 : Saos 2 (Human epithelial-like osteosarcoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 40 kDa
    Observed band size : 40 kDa
    Additional bands at : 37 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 2 minutes

References for Anti-BDKRB1 antibody (ab75120)

ab75120 has not yet been referenced specifically in any publications.

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Brain)
Specification Brain
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Dako Target Retrieval solution
Permeabilization No
Blocking step 5 minutes of peroxidase block then 10 minutes of protein block. These are ready-to-use reagents purchased from Dako as blocking agent for 15 minute(s) · Concentration: 100% · Temperature: 20°C
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Mr. Antibody Solutions

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Submitted May 19 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"