Anti-BDNF antibody [35928.11] (ab10505)
Key features and details
- Mouse monoclonal [35928.11] to BDNF
- Suitable for: ELISA, IHC-P, WB
- Reacts with: Human
- Isotype: IgG1
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-BDNF antibody [35928.11]
See all BDNF primary antibodies -
Description
Mouse monoclonal [35928.11] to BDNF -
Host species
Mouse -
Tested applications
Suitable for: ELISA, IHC-P, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant human BDNF (rhBDNF) expressed in Sf 21 insect cells.
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Positive control
- Brain tissue lysate
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General notes
Endotoxin: <10 ng/mg by LAL method.For BDNF, multiple WB bands are possible and expected. The human protein has 5 isoforms (precursors: 28 – 37 kDa) and can be glycosylated (Uniprot: http://www.uniprot.org/uniprot/P23560). The mature form is expected at ~14 kDa (monomer) and the dimer at ~28 kDa.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Constituent: PBS -
Concentration information loading...
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Purity
Protein A purified -
Purification notes
The antibody is purified by Protein A affinity chromatography. -
Clonality
Monoclonal -
Clone number
35928.11 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab10505 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ELISA |
Use at an assay dependent concentration.
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IHC-P |
Use at an assay dependent concentration.
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WB | (1) |
Use at an assay dependent concentration.
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Notes |
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ELISA
Use at an assay dependent concentration. |
IHC-P
Use at an assay dependent concentration. |
WB
Use at an assay dependent concentration. |
Target
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Function
During development, promotes the survival and differentiation of selected neuronal populations of the peripheral and central nervous systems. Participates in axonal growth, pathfinding and in the modulation of dendritic growth and morphology. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability. -
Tissue specificity
Brain. Highly expressed in hippocampus, amygdala, cerebral cortex and cerebellum. Also expressed in heart, lung, skeletal muscle, testis, prostate and placenta. -
Involvement in disease
Bulimia nervosa 2
Congenital central hypoventilation syndrome -
Sequence similarities
Belongs to the NGF-beta family. -
Post-translational
modificationsThe propeptide is N-glycosylated and glycosulfated.
Converted into mature BDNF by plasmin (PLG). -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 627 Human
- Omim: 113505 Human
- SwissProt: P23560 Human
- Unigene: 502182 Human
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Alternative names
- Abrineurin antibody
- ANON2 antibody
- BDNF antibody
see all
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (14)
ab10505 has been referenced in 14 publications.
- Allen CNS et al. Metabolic Reprogramming in HIV-Associated Neurocognitive Disorders. Front Cell Neurosci 16:812887 (2022). PubMed: 35418836
- Sun Q et al. microRNA-9 and -29a regulate the progression of diabetic peripheral neuropathy via ISL1-mediated sonic hedgehog signaling pathway. Aging (Albany NY) 12:11446-11465 (2020). PubMed: 32544883
- Zhang L et al. LncRNA Rik-203 Contributes to Sevoflurane Induced Neurotoxicity? Front Med (Lausanne) 7:353 (2020). PubMed: 32793611
- Jasim H et al. Daytime changes of salivary biomarkers involved in pain. J Oral Rehabil 47:843-850 (2020). PubMed: 32277715
- Jasim H et al. Saliva as a medium to detect and measure biomarkers related to pain. Sci Rep 8:3220 (2018). PubMed: 29459715