RabMAb

Anti-BDNF antibody [EP1293] (ab108383)

Overview

  • Product nameAnti-BDNF antibody [EP1293]See all BDNF primary antibodies ...
  • Description
    Rabbit monoclonal [EP1293] to BDNF
  • Tested applicationsWB, ICC/IF, IHC-Fr more details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive)

  • Positive control
    • C6, fetal brain, Human hippocampus cell lysates and recombinant protein; PC12 cells
  • General notesProduced under U.S. Patent No. 5,675,063

Properties

Applications

Our Abpromise guarantee covers the use of ab108383 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB 1/1000 - 1/10000. Detects a band of approximately 27-34 kDa (predicted molecular weight: 28 kDa). Increase dilution if high background is observed
ICC/IF 1/100 - 1/250.
IHC-Fr Use at an assay dependent concentration.
  • Application notesIs unsuitable for Flow Cyt,IHC-P or IP.
  • Target

    • FunctionDuring development, promotes the survival and differentiation of selected neuronal populations of the peripheral and central nervous systems. Participates in axonal growth, pathfinding and in the modulation of dendritic growth and morphology. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability.
    • Tissue specificityBrain. Highly expressed in hippocampus, amygdala, cerebral cortex and cerebellum. Also expressed in heart, lung, skeletal muscle, testis, prostate and placenta.
    • Involvement in diseaseCongenital central hypoventilation syndrome
    • Sequence similaritiesBelongs to the NGF-beta family.
    • Post-translational
      modifications
      The propeptide is N-glycosylated and glycosulfated.
      Converted into mature BDNF by plasmin (PLG).
    • Cellular localizationSecreted.
    • Target information above from: UniProt accession P23560 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links
    • Alternative names
      • Abrineurin antibody
      • BDNF antibody
      • BDNF antibody
      • BDNF_HUMAN antibody
      • Brain Derived Neurotrophic Factor antibody
      • Brain-derived neurotrophic factor antibody
      • MGC34632 antibody
      • Neurotrophin antibody
      see all

    Anti-BDNF antibody [EP1293] images

    • All lanes : Anti-BDNF antibody [EP1293] (ab108383) at 1/1000 dilution

      Lane 1 : C6 cell lysate
      Lane 2 : Fetal brain lysate
      Lane 3 : Human hippocampus lysate
      Lane 4 : Recombinant BDNF protein

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 28 kDa
    • Immunofluorescent staining of PC12 cells using ab108383 at a dilution of 1/100.
    • ab108383 staining BDNF in human lung tissue by Immunohistochemistry (Frozen sections).
      Tissue was fixed in methanol, permeabilized using 0.1% Saponin/PBS, blocked with 4% serum for 30 minutes at 25°C and then incubated with ab108383 at a 1/100 dilution for 14 hours at 4°C. The secondary used was a Texas Red conjugated goat anti-rabbit polyclonal used at a 1/100 dilution.

      See Abreview

    References for Anti-BDNF antibody [EP1293] (ab108383)

    ab108383 has not yet been referenced specifically in any publications.

    Product Wall

    Thank you for your enquiry. I am pleased to help answer your questions:

    1. I would like to reassure you that both ab108383 and ab6201 are succesfully tested and therefore covered by the Abcam guarantee in ICC-IF. Therefore, any BDNF antibody...

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    Application Western blot
    Sample Rat Tissue lysate - whole (cortex)
    Loading amount 30 µg
    Specification cortex
    Gel Running Conditions Reduced Denaturing (gel 10% SDS-PAGE)
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Username

    Dr. Karine Thibault

    Verified customer

    Submitted Feb 18 2013

    Application Immunohistochemistry (PFA perfusion fixed frozen sections)
    Sample Rat Tissue sections (cortex)
    Specification cortex
    Fixative Paraformaldehyde
    Antigen retrieval step None
    Permeabilization No
    Username

    Dr. Karine Thibault

    Verified customer

    Submitted Feb 18 2013

    Thank you for contacting us.
    We have quite a few antibodies for BDNF. The antibodies ab108319, ab108383 ab109049 or ab72439 are against mature BDNF however ab78367 would detect the pro form.
    I hope this information is helpful to you. Please d...

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    Thank you for your email.
    I'm glad to see that the antibody worked.
    Please do not hesitate to contact us if you need any more advice or information.
    Use our products? Submit an Abreview. Earn rewards!
    http://www.abcam.com/abreviews

    Thank you for your email.
    Yes, the image you sent looks good and I'm glad to hear that ab6721 gives satisfactory results.
    Please let me know if I can assist you in any other way.
    Use our products? Submit an Abreview. Earn rewards!
    h...

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    Thank you for your email and for sending the images.
    I am sorry to see that the suggestions have not helped to improve the staining results.
    I am not sure what has happened, but it seemed the lower primary antibody dilution reduces the specif...

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    Thank you for your reply.
    Please let me know your results. OK, if the manufacturer recommends 5-10 min, then you could go up to 10 min with the blocking step - to try if it improves the results and reduces the background.
    I hope this informat...

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    Thank you for your email and the images.
    The images do look good, except the background.
    As I had suggested in my earlier email today, maybe decreasing the primary antibody concentration to 1/500 or 1/1000 could help, while leaving the second...

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    Thank you for your email.
    You had said, the other secondary antibody you had used also gave you high background. I am wondering if the staining images look similar - using the other and this secondary antibody?
    Yes, I think decreasing the pri...

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