Anti-BDNF antibody [EP1293] (ab108383)

Overview

• Product nameAnti-BDNF antibody [EP1293]See all BDNF primary antibodies ...
• Description
  Rabbit monoclonal [EP1293] to BDNF
• Tested applicationsWB, ICC/IF, IHC-Fr more details
• Species reactivity
  Reacts with: Mouse, Rat, Human
  
• Immunogen

  Synthetic peptide (the amino acid sequence is considered to be commercially sensitive)

• Positive controlC6, fetal brain, Human hippocampus cell lysates and recombinant protein; PC12 cells
• General notesProduced under U.S. Patent No. 5,675,063

Properties

• FormLiquid
• Storage instructionsStore at -20°C. Stable for 12 months at -20°C
• Storage bufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
• PurityTissue culture supernatant
• Clonality Monoclonal
• Clone numberEP1293
• IsotypeIgG
• Research Areas
  • Metabolism
  • Types of disease
  • Diabetes

  • Metabolism
  • Types of disease
  • Obesity

  • Cardiovascular
  • Atherosclerosis
  • Thrombosis
  • Other

  • Cardiovascular
  • Atherosclerosis
  • Diabetes associated

  • Neuroscience
  • Neurology process
  • Neurodegenerative disease
  • Huntington's disease

  • Neuroscience
  • Neurology process
  • Growth and Development
  • Neurotrophins

  • Neuroscience
  • Neurology process
  • Neurodegenerative disease
  • Alzheimer's disease
  • Other

Applications

Target

• FunctionDuring development, promotes the survival and differentiation of selected neuronal populations of the peripheral and central nervous systems. Participates in axonal growth, pathfinding and in the modulation of dendritic growth and morphology. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability.
• Tissue specificityBrain. Highly expressed in hippocampus, amygdala, cerebral cortex and cerebellum. Also expressed in heart, lung, skeletal muscle, testis, prostate and placenta.
• Involvement in diseaseCongenital central hypoventilation syndrome (CCHS) [MIM:209880]: Rare disorder characterized by abnormal control of respiration in the absence of neuromuscular or lung disease, or an identifiable brain stem lesion. A deficiency in autonomic control of respiration results in inadequate or negligible ventilatory and arousal responses to hypercapnia and hypoxemia. Note=The disease is caused by mutations affecting the gene represented in this entry.
• Sequence similaritiesBelongs to the NGF-beta family.
• Post-translational
  modificationsThe propeptide is N-glycosylated and glycosulfated.
  Converted into mature BDNF by plasmin (PLG).
• Cellular localizationSecreted.

Target information above from: UniProt accession P23560 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 627 Human
  • Entrez Gene: 12064 Mouse
  • Entrez Gene: 24225 Rat
  • Omim: 113505 Human
  • SwissProt: P23560 Human
  • SwissProt: P21237 Mouse
  • SwissProt: P23363 Rat
  • Unigene: 502182 Human

  • Unigene: 1442 Mouse
  • Unigene: 11266 Rat

  see all

• Alternative names
  Abrineurin antibodyBDNF antibodyBDNF antibody

  BDNF_HUMAN antibodyBrain Derived Neurotrophic Factor antibodyBrain-derived neurotrophic factor antibodyMGC34632 antibodyNeurotrophin antibody

  see all

Anti-BDNF antibody [EP1293] images

• 

  Western blot - BDNF antibody [EP1293] (ab108383)
  All lanes : Anti-BDNF antibody [EP1293] (ab108383) at 1/1000 dilution
  
  Lane 1 : C6 cell lysate
  Lane 2 : Fetal brain lysate
  Lane 3 : Human hippocampus lysate
  Lane 4 : Recombinant BDNF protein
  
  Lysates/proteins at 10 µg per lane.
  
  
  Predicted band size : 28 kDa
  
• 

  Immunocytochemistry/ Immunofluorescence - BDNF antibody [EP1293] (ab108383)
  Immunofluorescent staining of PC12 cells using ab108383 at a dilution of 1/100.
• 

  Immunohistochemistry (Frozen sections) - Anti-BDNF antibody [EP1293] (ab108383)Image courtesy of an anonymous Abreview.
  ab108383 staining BDNF in human lung tissue by Immunohistochemistry (Frozen sections).
  
  Tissue was fixed in methanol, permeabilized using 0.1% Saponin/PBS, blocked with 4% serum for 30 minutes at 25°C and then incubated with ab108383 at a 1/100 dilution for 14 hours at 4°C. The secondary used was a Texas Red conjugated goat anti-rabbit polyclonal used at a 1/100 dilution.

  See Abreview