Anti-BDNF antibody [EPR1292] (ab108319)
- Product nameAnti-BDNF antibody [EPR1292]See all BDNF primary antibodies ...
- DescriptionRabbit monoclonal [EPR1292] to BDNF
- Tested applicationsWB, IHC-P more details
- Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide (Human)
- Positive controlWB: U87-MG, C6, SH-SY5Y, Neuron-2a, PC-12 cell lysate. IHC-P: Human brain tissue.
- General notesProduced under U.S. Patent No. 5,675,063.
- Storage instructionsStore at -20°C. Stable for 12 months at -20°C
- Storage bufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
- PurityTissue culture supernatant
- Clonality Monoclonal
- Clone numberEPR1292
Our Abpromise guarantee covers the use of ab108319 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||WB: 1/1000 - 1/10000. Predicted molecular weight: 28 kDa.|
|IHC-P||IHC-P: 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Heat up to 98 degrees C, below boiling, and then let cool for 10-20 min.|
- FunctionDuring development, promotes the survival and differentiation of selected neuronal populations of the peripheral and central nervous systems. Participates in axonal growth, pathfinding and in the modulation of dendritic growth and morphology. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability.
- Tissue specificityBrain. Highly expressed in hippocampus, amygdala, cerebral cortex and cerebellum. Also expressed in heart, lung, skeletal muscle, testis, prostate and placenta.
- Involvement in diseaseCongenital central hypoventilation syndrome (CCHS) [MIM:209880]: Rare disorder characterized by abnormal control of respiration in the absence of neuromuscular or lung disease, or an identifiable brain stem lesion. A deficiency in autonomic control of respiration results in inadequate or negligible ventilatory and arousal responses to hypercapnia and hypoxemia. Note=The disease is caused by mutations affecting the gene represented in this entry.
- Sequence similaritiesBelongs to the NGF-beta family.
modificationsThe propeptide is N-glycosylated and glycosulfated.
Converted into mature BDNF by plasmin (PLG).
- Cellular localizationSecreted.
- Abrineurin antibodyBDNF antibodyBDNF_HUMAN antibody
- Brain Derived Neurotrophic Factor antibodyBrain-derived neurotrophic factor antibodyMGC34632 antibodyNeurotrophin antibody
Anti-BDNF antibody [EPR1292] images
Immunohistochemical analysis of paraffin-embedded Human brain tissue using ab108319 at 1/100
Immunohistochemical analysis of brain tissue from sedentary (SED) or exercised rats (EX), staining BDNF with ab108319.
Antigen retrieval was performed by enzymatic method at room temperature. Tissue was permeabilized with 0.1% Triton X-100 and blocked with 10% goat serum. Samples were incubated with primary antibody (1/100) for 3 days at 3°C. An AlexaFluor®568-conjugated goat anti-rabbit IgG (1/1500) was used as the secondary antibody.
References for Anti-BDNF antibody [EPR1292] (ab108319)
This product has been referenced in:
- Quirié A et al. Effect of stroke on arginase expression and localization in the rat brain. Eur J Neurosci 37:1193-202 (2013). Rat . Read more (PubMed: 23311438) »
- Quirié A et al. Comparative effect of treadmill exercise on mature BDNF production in control versus stroke rats. PLoS One 7:e44218 (2012). WB, IHC-FoFr ; Rat . Read more (PubMed: 22962604) »