Anti-BDNF antibody [EPR1292] (ab108319)

Overview

• Product nameAnti-BDNF antibody [EPR1292]See all BDNF primary antibodies ...
• Description
  Rabbit monoclonal [EPR1292] to BDNF
• Tested applicationsWB, IHC-P more details
• Species reactivity
  Reacts with: Mouse, Rat, Human
  
• Immunogen

  Synthetic peptide (Human)

• Positive controlWB: U87-MG, C6, SH-SY5Y, Neuron-2a, PC-12 cell lysate. IHC-P: Human brain tissue.
• General notesProduced under U.S. Patent No. 5,675,063.

Properties

• FormLiquid
• Storage instructionsStore at -20°C. Stable for 12 months at -20°C
• Storage bufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
• PurityTissue culture supernatant
• Clonality Monoclonal
• Clone numberEPR1292
• IsotypeIgG
• Research Areas
  • Metabolism
  • Types of disease
  • Diabetes

  • Metabolism
  • Types of disease
  • Obesity

  • Cardiovascular
  • Atherosclerosis
  • Thrombosis
  • Other

  • Cardiovascular
  • Atherosclerosis
  • Diabetes associated

  • Neuroscience
  • Neurology process
  • Growth and Development
  • Neurotrophins

  • Neuroscience
  • Neurology process
  • Neurodegenerative disease
  • Alzheimer's disease
  • Other

  • Neuroscience
  • Neurology process
  • Neurodegenerative disease
  • Huntington's disease

Applications

Target

• FunctionDuring development, promotes the survival and differentiation of selected neuronal populations of the peripheral and central nervous systems. Participates in axonal growth, pathfinding and in the modulation of dendritic growth and morphology. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability.
• Tissue specificityBrain. Highly expressed in hippocampus, amygdala, cerebral cortex and cerebellum. Also expressed in heart, lung, skeletal muscle, testis, prostate and placenta.
• Involvement in diseaseCongenital central hypoventilation syndrome (CCHS) [MIM:209880]: Rare disorder characterized by abnormal control of respiration in the absence of neuromuscular or lung disease, or an identifiable brain stem lesion. A deficiency in autonomic control of respiration results in inadequate or negligible ventilatory and arousal responses to hypercapnia and hypoxemia. Note=The disease is caused by mutations affecting the gene represented in this entry.
• Sequence similaritiesBelongs to the NGF-beta family.
• Post-translational
  modificationsThe propeptide is N-glycosylated and glycosulfated.
  Converted into mature BDNF by plasmin (PLG).
• Cellular localizationSecreted.

Target information above from: UniProt accession P23560 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 627 Human
  • Entrez Gene: 12064 Mouse
  • Entrez Gene: 24225 Rat
  • Omim: 113505 Human
  • SwissProt: P23560 Human
  • SwissProt: P21237 Mouse
  • SwissProt: P23363 Rat
  • Unigene: 502182 Human

  • Unigene: 1442 Mouse
  • Unigene: 11266 Rat

  see all

• Alternative names
  Abrineurin antibodyBDNF antibodyBDNF_HUMAN antibody

  Brain Derived Neurotrophic Factor antibodyBrain-derived neurotrophic factor antibodyMGC34632 antibodyNeurotrophin antibody

  see all

Anti-BDNF antibody [EPR1292] images

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - BDNF antibody [EPR1292] (ab108319)
  Immunohistochemical analysis of paraffin-embedded Human brain tissue using ab108319 at 1/100
• 

  Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-BDNF antibody [EPR1292] (ab108319)Image from Quirié A et al., PLoS One. 2012;7(9):e44218. doi: 10.1371/journal.pone.0044218. Epub 2012 Sep 4.; Fig 5.; September 4, 2012, PLoS ONE 7(9): e44218.
  Immunohistochemical analysis of brain tissue from sedentary (SED) or exercised rats (EX), staining BDNF with ab108319.
  
  Antigen retrieval was performed by enzymatic method at room temperature. Tissue was permeabilized with 0.1% Triton X-100 and blocked with 10% goat serum. Samples were incubated with primary antibody (1/100) for 3 days at 3°C. An AlexaFluor®568-conjugated goat anti-rabbit IgG (1/1500) was used as the secondary antibody.