• Product nameAnti-BDNF antibody [EPR1292]
    See all BDNF primary antibodies
  • Description
    Rabbit monoclonal [EPR1292] to BDNF
  • SpecificityThis product may cross react with the following family members: NGF beta, neurotrophin 3, neurotrophin 4.
  • Tested applicationsSuitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (Human)

  • Positive control
    • WB: U87-MG, C6, SH-SY5Y, Neuron-2a, PC-12 cell lysate, brain tissue lysate. IHC-P: Human brain tissue.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    For BDNF, multiple WB bands are possible and expected. The human protein has 5 isoforms (precursors: 28 – 37 kDa) and can be glycosylated (Uniprot: http://www.uniprot.org/uniprot/P23560). The mature form is expected at ~14 kDa (monomer) and the dimer at ~28 kDa.



Our Abpromise guarantee covers the use of ab108319 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 15 kDa.Can be blocked with BDNF peptide (ab182199).
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Heat up to 98 degrees C, below boiling, and then let cool for 10-20 min.
  • Application notesIs unsuitable for Flow Cyt,ICC or IP.
  • Target

    • FunctionDuring development, promotes the survival and differentiation of selected neuronal populations of the peripheral and central nervous systems. Participates in axonal growth, pathfinding and in the modulation of dendritic growth and morphology. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability.
    • Tissue specificityBrain. Highly expressed in hippocampus, amygdala, cerebral cortex and cerebellum. Also expressed in heart, lung, skeletal muscle, testis, prostate and placenta.
    • Involvement in diseaseBulimia nervosa 2
      Congenital central hypoventilation syndrome
    • Sequence similaritiesBelongs to the NGF-beta family.
    • Post-translational
      The propeptide is N-glycosylated and glycosulfated.
      Converted into mature BDNF by plasmin (PLG).
    • Cellular localizationSecreted.
    • Information by UniProt
    • Database links
    • Alternative names
      • Abrineurin antibody
      • ANON2 antibody
      • BDNF antibody
      • BDNF_HUMAN antibody
      • Brain Derived Neurotrophic Factor antibody
      • Brain-derived neurotrophic factor antibody
      • BULN2 antibody
      • MGC34632 antibody
      • Neurotrophin antibody
      see all

    Anti-BDNF antibody [EPR1292] images

    • Immunohistochemical analysis of brain tissue from sedentary (SED) or exercised rats (EX), staining BDNF with ab108319.

      Antigen retrieval was performed by enzymatic method at room temperature. Tissue was permeabilized with 0.1% Triton X-100 and blocked with 10% goat serum. Samples were incubated with primary antibody (1/100) for 3 days at 3°C. An AlexaFluor®568-conjugated goat anti-rabbit IgG (1/1500) was used as the secondary antibody.
    • Immunohistochemical analysis of paraffin-embedded Human brain tissue using ab108319 at 1/100
    • All lanes : Anti-BDNF antibody [EPR1292] (ab108319) at 1/1000 dilution

      Lane 1 : Human hippocampus lysate
      Lane 2 : Rat hippocampus lysate
      Lane 3 : Mouse hippocampus lysate

      Lysates/proteins at 20 µg per lane.

      Gt anti Rb IR680 at 1/10000 dilution

      Performed under reducing conditions.

      Predicted band size : 15 kDa
      Additional bands at : 15 kDa (possible mature (processed) protein),28 kDa (possible multimer),35 kDa,45 kDa (possible immature (unprocessed)). We are unsure as to the identity of these extra bands.

      This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using Licor blocking buffer before being incubated with ab108319 (1/1000) overnight at 4°C. Ab8245 (mouse anti-GAPDH; 0.05 ug/mL) was included as a loading control. Antibody binding was detected using goat anti-rabbit IgG IR-680 (green) and goat anti-mouse IgG IR800 (red) at a 1:10,000 dilution for 1hr at room temperature and then imaged using the Licor Odyssey CLx

    References for Anti-BDNF antibody [EPR1292] (ab108319)

    This product has been referenced in:
    • Monnier A  et al. Brain-derived neurotrophic factor of the cerebral microvasculature: a forgotten and nitric oxide-dependent contributor of brain-derived neurotrophic factor in the brain. Acta Physiol (Oxf) N/A:N/A (2016). Rat . Read more (PubMed: 27364224) »
    • Liu T  et al. Cannabinoid-Elicited Conditioned Place Preference in a Modified Behavioral Paradigm. Biol Pharm Bull 39:747-53 (2016). WB ; Rat . Read more (PubMed: 26935020) »

    See all 11 Publications for this product

    Product Wall

    Thank you for contacting us.
    We have quite a few antibodies for BDNF. The antibodies ab108319, ab108383 ab109049 or ab72439 are against mature BDNF however ab78367 would detect the pro form.
    I hope this information is helpful to you. Please d...

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    Thank you for contacting us and for your interest in our products. I think ab108319 (www.abcam.com/ab108319) or ab108383 (www.abcam.com/ab108383) would be suitable since you are looking for a monoclonal antibody. However, please note that none of t...

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