Overview

  • Product nameAnti-BDNF antibody [EPR1292]
    See all BDNF primary antibodies
  • Description
    Rabbit monoclonal [EPR1292] to BDNF
  • Tested applicationsWB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (Human)

  • Positive control
    • WB: U87-MG, C6, SH-SY5Y, Neuron-2a, PC-12 cell lysate. IHC-P: Human brain tissue.
  • General notesProduced under U.S. Patent No. 5,675,063.

Properties

Applications

Our Abpromise guarantee covers the use of ab108319 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 28 kDa.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Heat up to 98 degrees C, below boiling, and then let cool for 10-20 min.
  • Application notesIs unsuitable for Flow Cyt,ICC or IP.
  • Target

    • FunctionDuring development, promotes the survival and differentiation of selected neuronal populations of the peripheral and central nervous systems. Participates in axonal growth, pathfinding and in the modulation of dendritic growth and morphology. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability.
    • Tissue specificityBrain. Highly expressed in hippocampus, amygdala, cerebral cortex and cerebellum. Also expressed in heart, lung, skeletal muscle, testis, prostate and placenta.
    • Involvement in diseaseCongenital central hypoventilation syndrome
    • Sequence similaritiesBelongs to the NGF-beta family.
    • Post-translational
      modifications
      The propeptide is N-glycosylated and glycosulfated.
      Converted into mature BDNF by plasmin (PLG).
    • Cellular localizationSecreted.
    • Information by UniProt
    • Database links
    • Alternative names
      • Abrineurin antibody
      • BDNF antibody
      • BDNF_HUMAN antibody
      • Brain Derived Neurotrophic Factor antibody
      • Brain-derived neurotrophic factor antibody
      • MGC34632 antibody
      • Neurotrophin antibody
      see all

    Anti-BDNF antibody [EPR1292] images

    • Immunohistochemical analysis of paraffin-embedded Human brain tissue using ab108319 at 1/100
    • Immunohistochemical analysis of brain tissue from sedentary (SED) or exercised rats (EX), staining BDNF with ab108319.

      Antigen retrieval was performed by enzymatic method at room temperature. Tissue was permeabilized with 0.1% Triton X-100 and blocked with 10% goat serum. Samples were incubated with primary antibody (1/100) for 3 days at 3°C. An AlexaFluor®568-conjugated goat anti-rabbit IgG (1/1500) was used as the secondary antibody.

    References for Anti-BDNF antibody [EPR1292] (ab108319)

    This product has been referenced in:
    • Rodier M  et al. Exogenous t-PA administration increases hippocampal mature BDNF levels. plasmin- or NMDA-dependent mechanism? PLoS One 9:e92416 (2014). WB ; Rat . Read more (PubMed: 24670989) »
    • Quirié A  et al. Effect of stroke on arginase expression and localization in the rat brain. Eur J Neurosci 37:1193-202 (2013). Rat . Read more (PubMed: 23311438) »

    See all 4 Publications for this product

    Product Wall

    Thank you for contacting us.
    We have quite a few antibodies for BDNF. The antibodies ab108319, ab108383 ab109049 or ab72439 are against mature BDNF however ab78367 would detect the pro form.
    I hope this information is helpful to you. Please d...

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    Thank you for contacting us and for your interest in our products. I think ab108319 (www.abcam.com/ab108319) or ab108383 (www.abcam.com/ab108383) would be suitable since you are looking for a monoclonal antibody. However, please note that none of t...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"