Overview

  • Product nameAnti-BDNF antibodySee all BDNF primary antibodies ...
  • Description
    Goat polyclonal to BDNF
  • SpecificitySpecific for mature BDNF.
  • Tested applicationsWB, IHC-P, IHC-FoFr more details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptides (a dimer) from mature Rat BDNF conjugated to an immunogenic carrier protein

Properties

Applications

Our Abpromise guarantee covers the use of ab101751 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB 1/300 - 1/2000. Predicted molecular weight: 28 kDa. This antibody has been tested in Western blot against the recombinant peptide used as an immunogen. We have no data on detection of endogenous protein.
IHC-P 1/300 - 1/2000. Antigen retrieval is recommended.
IHC-FoFr 1/200 - 1/3000. We recommend incubating overnight with shaking. Zamboni fixative is also suitable with this antibody.

Target

  • FunctionDuring development, promotes the survival and differentiation of selected neuronal populations of the peripheral and central nervous systems. Participates in axonal growth, pathfinding and in the modulation of dendritic growth and morphology. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability.
  • Tissue specificityBrain. Highly expressed in hippocampus, amygdala, cerebral cortex and cerebellum. Also expressed in heart, lung, skeletal muscle, testis, prostate and placenta.
  • Involvement in diseaseCongenital central hypoventilation syndrome
  • Sequence similaritiesBelongs to the NGF-beta family.
  • Post-translational
    modifications
    The propeptide is N-glycosylated and glycosulfated.
    Converted into mature BDNF by plasmin (PLG).
  • Cellular localizationSecreted.
  • Target information above from: UniProt accession P23560 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
  • Alternative names
    • Abrineurin antibody
    • BDNF antibody
    • BDNF antibody
    • BDNF_HUMAN antibody
    • Brain Derived Neurotrophic Factor antibody
    • Brain-derived neurotrophic factor antibody
    • MGC34632 antibody
    • Neurotrophin antibody
    see all

References for Anti-BDNF antibody (ab101751)

ab101751 has not yet been referenced specifically in any publications.

Product Wall

Thank you for contacting us.

All three products seem to be suitable for your application and are reacting with rat, although there are a few differences to be considered.

ab101748
This antibody positively tested for IHC on frozen ...

Read More

Thank you for sending the additional information, and I am glad that we found a way to solve this issue.
I've issued credit note #***. If you have any questions about this credit, please contact creditcontrol@abcam.com.
Thank you for letting ...

Read More

Thank you for your reply, and I hope that the customer is feeling much better now.
To address your points:
1) I certainly want to make sure that the customer is properly compensated for the malfunctioning ab101751, and we do have a couple of ...

Read More

Thanks for your reply.
The voucher can actually only be used for a primary antibody. I'd be happy to send the customer a free of charge replacement with a fluorescent secondary antibody if they would like. If so, please let me know which they pref...

Read More

Thank you for your reply.
The vial of ab101751 was actually purchased with an Abreview testing voucher, and I am unable to credit this as it was a free product. If there is no other antibody that the customer would like at this time, I can issue h...

Read More

Thank you for contacting us and letting us know about the trouble with this antibody.
I have asked the production lab for the protocol that they used to test this antibody with frozen tissue, and I apologize for the delay in getting this informati...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"