Anti-BDNF antibody (ab223354)
Key features and details
- Rabbit polyclonal to BDNF
- Suitable for: ICC/IF, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-BDNF antibody
See all BDNF primary antibodies -
Description
Rabbit polyclonal to BDNF -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide corresponding to Human BDNF (N terminal).
Database link: P23560 -
Positive control
- WB: HeLa and HEK-293T cell lysates; Mouse brain lysate. ICC/IF: SK-N-BE cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab223354 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
1/100.
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WB |
1/1000. Detects a band of approximately 27.9 kDa (predicted molecular weight: 27.9 kDa).
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Notes |
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ICC/IF
1/100. |
WB
1/1000. Detects a band of approximately 27.9 kDa (predicted molecular weight: 27.9 kDa). |
Target
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Function
During development, promotes the survival and differentiation of selected neuronal populations of the peripheral and central nervous systems. Participates in axonal growth, pathfinding and in the modulation of dendritic growth and morphology. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability. -
Tissue specificity
Brain. Highly expressed in hippocampus, amygdala, cerebral cortex and cerebellum. Also expressed in heart, lung, skeletal muscle, testis, prostate and placenta. -
Involvement in disease
Bulimia nervosa 2
Congenital central hypoventilation syndrome -
Sequence similarities
Belongs to the NGF-beta family. -
Post-translational
modificationsThe propeptide is N-glycosylated and glycosulfated.
Converted into mature BDNF by plasmin (PLG). -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 627 Human
- Entrez Gene: 12064 Mouse
- Omim: 113505 Human
- SwissProt: P23560 Human
- SwissProt: P21237 Mouse
- Unigene: 502182 Human
- Unigene: 1442 Mouse
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Alternative names
- Abrineurin antibody
- ANON2 antibody
- BDNF antibody
see all
Images
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4% formaldehyde-fixed SK-N-BE cells stained for BDNF (green) using ab223354 at 1/100 dilution in ICC/IF. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1/1000, 1/5000 for 60 min RT, 5 min RT.
(A) DAPI nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) ab223354 (D) Merge.
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Anti-BDNF antibody (ab223354) at 1/1000 dilution + Mouse brain lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG HRP at 1/2000 dilution
Predicted band size: 27.9 kDa
Observed band size: 27.9 kDa
Exposure time: 12 minutesBlocking buffer: 5% milk in TBST.
Color Development: TMB solution.
Primary and secondary antibodies were incubated for 1 hour at RT.
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All lanes : Anti-BDNF antibody (ab223354) at 1/1000 dilution
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG HRP at 1/2000 dilution
Predicted band size: 27.9 kDa
Observed band size: 27.9 kDa
Exposure time: 12 minutesBlocking buffer: 5% milk in TBST.
Color Development: TMB solution.
Primary and secondary antibodies were incubated for 1 hour at RT.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab223354 has been referenced in 1 publication.
- Ge X et al. Increased Microglial Exosomal miR-124-3p Alleviates Neurodegeneration and Improves Cognitive Outcome after rmTBI. Mol Ther 28:503-522 (2020). PubMed: 31843449